KSU-CVM Shared Resource Scheduler

Overview of Services

Kansas State University College of Veterinary Medicine is committed to providing exceptional teaching, impactful research, outstanding services, and extraordinary graduates through support of institutional shared resources. By making research resources and infrastructure available to the larger research community, we facilitate partnerships and interdisciplinary collaborations that improve efficiency and engagement for experimental outcomes. Our goal is to target collaborative research opportunities and funding in areas of basic research, animal infectious disease, food safety and security, and comparative translational medicine. The College of Veterinary Medicine looks to provide advanced instrumentation and technical support to foster entrepreneurship and innovative transdisciplinary research.

AMP Core

• The AMP Core provides personalized, project-specific support and expertise to facilitate the use of animal models and pathological services. Existing infrastructure includes a new laser capture microdissection instrument. The AMP Core will also produce hybridomas and hyper-immune sera for the development of diagnostic tests essential to COBRE research projects.

Confocal Core

• Originally founded in 2002 through an NIH-COBRE, the Confocal Core offers researchers a choice of three laser scanning confocal microscopes including LSM 700, 800 and 880 Airyscan to support all manner of imaging needs. Techniques supported include multi-channel imaging, quantitative analysis, colocalization, FRET, FRAP, 3D reconstruction, microfluorometry and super-res on both live and fixed cells. Provisions are available for live cell perfusion experiments. The facility also includes access to tissue-sectioning equipment to include a cryostat and Vibratome.

Flow Cytometry Core

• Offers cytometer capabilities to perform polychromatic analysis via the four-laser/14-detector capabilities. The facility also provides access to cell sorter resources for up to three lasers and an assortment of filters and detectors to quickly sort a variety of cells either chilled or at physiologic temperature into a multitude of culture receptacles for further processing.

MCB Core

• The MCB Core provides technological support and significant research equipment/resources to all biomedical researchers at K-State and within the Regional Scientific Network. In addition, the MCB Core Laboratory offers training to faculty, staff, postdocs, graduate, and undergraduate students on the use of MCB Core equipment and technologies.

Next-Gen Sequencing

• Since 2013, the Next-Generation Sequencing (NGS) Core Lab at the K-State College of Veterinary Medicine has provided short and long-read sequencing services using Illumina, Nanopore, and PacBio (2020) platform technologies. Whole genome sequencing, RNA-seq, metagenomic sequencing, amplicon sequencing, bacterial and viral sequencing, and the supporting bioinformatics analyses are among the services offered by the NGS Core Lab.

Nanotechnology Core

• The Nanotechnology Core facility in the Department of Anatomy and Physiology in the KSU College of Veterinary Medicine (CVM) is a multiuser facility serving CVM, KSU campus, and research and commercial institutes outside campus. A well-equipped tissue culture facility can provide service for multiple investigators. A dedicated microscopic imaging room if available and includes bright field, dark field, and fluorescence microscopy. The core has two plate readers capable of absorbance, fluorescence, and luminescence based assays. The range of molecular biological instrumentation includes a QuantStudio 7 Flex, a Luminex 200, and a Chirascan CD spectrophotometer, allowing the core lab to be used for RT-PCR, ELISA, chemokine and cytokine assays, and thermodynamic and 3D structural characterization of proteins. State-of-the-art nanoparticle characterization equipment, such as Zetasizer, Nanosight, and a CPS high resolution disk centrifuge are available for measuring size and concentration of nanoparticles in suspension. A fully equipped surgical suite for small animals (up to 100 lb.) and an IVIS bio-imaging system facilitate in vivo experiments.

Analytical Pharmacology

• The Analytical Pharmacology Lab located in 330 Coles is equipped with two state-of- the-art systems from Waters Corp. The Xevo TQ-S is a tandem quadrupole mass spectrometer designed for quantitative UPLC -MS/MS. The Xevo TQ-Absolute is a tandem quadrupole mass spectrometer designed for UHPLC/MS/MS and is 15 times more sensitive than the TQ-S. Only our in-house, trained, analytical chemists have access to these instruments. Sample analysis and method development is done on fee-for-service basis. Types of samples we can currently analyze for drug quantification or blood biomarkers include blood plasma, serum, tissue, as well as animal feed materials.

Leadership

Dr. Igor Morozov | Director of AMP | imorozov@vet.k-state.edu

Dr. Natasha Gaudreault | Assistant Director of AMP | nng5757@vet.k-state.edu

Joel Sanneman | Confocal Core & Flow Cytometry Core Manager | 785-341-2145 | jsanneman@vet.k-state.edu

Dr. Juergen Richt | MCB Core Director | 785-532-2793 | jricht@vet.k-state.edu

Dr. Jayme Souza-Neto | MCB Core Assistant Director | jsouzameto@vet.k-state.edu

Dr. Waithaka Mwangi | MCB Core Associate Director

Mike Hays | MCB Core Manager | 785-632-4631 | hays@vet.k-state.edu

Dr. Tej Shrestha | Nanotechnology Core Manager | tbs3@vet.k-state.edu

Dr. Hans Coetzee | Director of LCMS Core

Misty Bear | LCMS Core Manager | 785-532-4688 | mdf2130@vet.k-state.edu

Location and hours of operation

Links and Resources

• https://ceezad.org
• https://www.k-state.edu/cezid

Contacts