UMass Boston Chemistry Core

Overview of Services

Proteomics Core

The University of Massachusetts Boston Proteomics Core offers a full range of proteomic services using an Orbitrap Fusion Lumos Tribrid mass spectrometer at highly competitive rates. Our services range from sample preparation, bottom-up protein identification, quantification of expression levels using both label free and TMT multiplexing strategies, PTM analysis, 2D fractionation, and top-down proteomic analysis. Our instrumentation offers unique flexibility in designing proteomic experiments to provide the best outcome for your project. The director of the core, Professor Jason Evans, has over 25 years of experience in the field of mass spectrometry and proteomics, and as a facility we have been servicing the proteomics needs of multiple principle investigators at the University of Massachusetts Boston since the Spring of 2016. We offer unmatched customer service, taking the time to consult with you to learn your needs and formulate the best plan for analyzing your samples, so that you get the most out of the resulting data. We work very hard to keep our turn-around times as short as possible. In addition, as a core facility at the University of Massachusetts Boston, we are currently in a unique position to offer our services to small businesses with less than 10 employees at a 75% discount and to small businesses with less than 50 employees a 50% discount from subsidies provided though the state of Massachusetts.

Biophysical Instrumentation Core (BIC) Facility

The Biophysical Instrumentation Core (BIC) Facility was recently established at UMass Boston. Funded by the Massachusetts Life Sciences Center and UMass Boston, the core's mission is to support academic and industry users with questions to probe the function of biomolecules. The core is equipped with instruments to aid researchers in characterizing molecular interactions, sample integrity, stability, structure, and more.  We are located within the Integrated Sciences Complex at Umass Boston and accessible by car (with on campus parking) or by the red line JFK stop (UMass Boston has a shuttle from the T stop to campus). The core is open to both internal and external users. Members may be trained to use instruments or contact the core to schedule submitting samples. Please email us at BIC@umb.edu so that we can best help you get set up to use the BIC. 

Services:  high-throughput differential scanning calorimetry (TA Instruments NanoDSC with autosampler), isothermal titration calorimetry (TA Instruments Affinity ITC), high-throughput surface plasmon resonance (Cytiva Biacore 8K), microscale capillary thermophoresis (NanoTemper Monolith, dual channel: PicoRed and Unlabeled), high-throughput circular dichroism (JASCO HTCD and J-1500 with flow cell or 6-cuvette adaptors, both with thermal ramp capability), fluorescence spectroscopy (JASCO FP-8350, with 4-cuvette adaptor, with thermal ramp, microvolume, and fluorescence anisotropy capabilities), Agilent HPLC with integrated Wyatt multiangle light scattering with RI determination (Wyatt Dawn and OptiLab), and dynamic light scattering (Wyatt NanoStar II, with in-line SEC-MALS or single cuvette batch modes available).

Scanning Electron Microscope (SEM)

ZEISS Sigma500VP FESEM is an advanced field emission SEM that produces exceptional images at high and low accelerating voltages. Together with its analytical capabilities, this instrument is suitable for a wide range of applications. The instrument owns a high resolution filed emission column with a high stability Schottky field gun, the beam current ranges from 1 pA to 400 nA, and the acceleration voltage range is from 200 eV to 30 keV.

Detectors: In-lens secondary electron, Everhart-Thornley secondary electron, variable pressure secondary electron, backscattered electron. 

The instrument is equipped with Oxford energy dispersive spectroscopy (EDS) system for elemental and chemical analysis of a sample inside the SEM. 

Leadership

Proteomics Core

Jason Evans, PhD | Director

Biophysical Instrumentation Core (BIC) Facility

Daniel Dowling, PhD | Director

Kimberly Hamad-Schifferli, PhD | Co-Director

Mohamed Gharbi, PhD | Co-Director

Alexey Veraksa, PhD | Co-Director

Scanning Electron Microscope (SEM)

Niya Sa, PhD | Director

Location and hours of operation

Proteomics Core

 Biophysical Instrumentation Core (BIC) Facility

Scanning Electron Microscope (SEM)

Links and Resources

• Proteomics Core
• https://www.umb.edu/academics/csm/faculty_staff/jason_evans
• Biophysical Instrumentation Core (BIC) Facility
• FESEM Core Site
• https://saniyabnu.wixsite.com/niyasa

Contacts