DLAR Imaging Core

Overview of Services

Welcome to the Department of Laboratory Animal Resources (DLAR) Imaging Core which provides imaging services for live animals, tissues and cells to support research performed at the University of Maryland, College Park campus. The core allows investigators to perform multiple imaging modalities which include the following equipment and Technology:

  • Fluorescent and chemiluminescent imaging utilizing the IVIS Spectrum

  • Rodent Ultrasonography utilizing the Vevo 3100 ultrasound unit

  • Rodent Computed Tomography with SPECT/PET capability utilizing the Albira Si CT-SPECT-PET unit

  • Radiography and fluoroscopic imaging using the OEC One Mobile C-arm

  • Multiphoton microscopy using the FVMPE-RS multiphoton laser scanning microscope

  • Irradiation service using the X-RAD 320 are also available

 

The A James Clark building also houses a state of the art vivarium and DLAR offices on the 6th floor. The vivarium contains rodent housing, large animal housing, procedure rooms, a dedicated surgery suite, cage wash support areas, and quarantine housing. DLAR oversees this facility and offers a variety of services including rodent housing and husbandry care, large animal housing and husbandry care, steam sterilization of materials, and ethylene oxide sterilization of materials.  Surgery and procedure room space is available and anesthesia machines are available for use.

  • Compac5  Active Scavenging Mobile Multi-patient Anesthesia Unit for rodents

  • RC2 Passive Scavenging Portable Anesthesia Machines for rodents

  • Ethylene Oxide gas sterilization services for instruments and materials that cannot be steam sterilized

  • Steam sterilization services for surgical instruments and materials

  • Ionized hydrogen peroxide disinfection services using STERAMIST equipment 

Location and hours of operation

Hours

Location

Monday-Friday

8:30AM-4:30PM    

University of Maryland College Park            6th Floor, A. James Clark/Bldg. 429          8278 Paint Branch Drive                      College Park, MD 20742
 
Main contact email address: DLARIC@umd.edu

Available Imaging Equipment

 

 

IVIS Spectrum Imaging System

The IVIS Spectrum Imaging System enables high-sensitivity non-invasive in vivo fluorescent and bioluminescent imaging. This system features high resolution to 20 microns with a 3.9 cm field of view, high throughput imaging of up to 5 mice with a 23 cm field of view, and twenty eight high efficiency filters which span from 430-850nm for imaging across the blue to near infrared wavelength region. Spectral unmixing tools allow for the separation of signals from multiple fluorescent reporters within the same animal enabling the use of multiple reporters. An optical switch in the fluorescence illumination path allows illumination in either reflection-mode (epi-illumination/above) or transmission-mode (trans- illumination/below). Living Image Acquisition/Analysis Package software allows for advanced quantification and analysis of data. 

 

 

Vevo 3100 Ultrasound

The Vevo 3100 micro-ultrasound imaging system combines ultra high-frequency ultrasound imaging operating up to 70 MHz and imaging up to 300 frames per second. The system is equipped with complete animal setup and handling to achieve noninvasive in vivo imaging under accurate physiological conditions (temperature-controlled heated stage, gas anesthesia, with EKG, temperature, and respiratory rate monitoring). The system has an injection setup mounted on dedicated rail-system extensions to enable Y-axis stage adjustment that can be used to assist in image-guided injections. Three transducers are available:
• MX201: 18 Mhz MX series transducer: 100 micron resolution for rats > 400 g for cardiovascular & abdominal imaging. 
• MX250: 24 MHz MicroScan transducer: 75 micron resolution for rats <400 g for cardiovascular & abdominal imaging, large tumor imaging, all contrast applications. 
• MX550D: 55 MHz MicroScan transducer: 40 micron resolution. High-resolution cardiovascular imaging, mouse abdominal, reproductive, mouse/rat embryology, tumor imaging (up to 14 mm in diameter), mouse vascular imaging. 

 

 

Albira Si PET/SPECT/CT imaging system

The Albira Si is a trimodal imaging system that integrates Positron Emission Tomography (PET), Single-Photon Emission Computed Tomography (SPECT), and X-ray Computed Tomography (CT) within one fully x-ray shielded and stand-alone imaging platform. The fully automated animal handling system enables automatic co-registration of images. 

• Triple-ring Albira Si PET detector system with typical performance characteristics: FOV- transaxial 80mm, axial 148/286 mm (single position/moving table); spatial resolution up to 0.7 mm; sensitivity of 12%. 
• Albira Si SPECT imaging detector based on two S108 gamma cameras using continuous Csl(Na) crystals, four 8x8 PSPMT arrays and exchangeable collimators with typical performance characteristics using a single pinhole SPECT collimator: max resolution FWHM ~0.7mm, energy range- up to ~175keV; average energy resolution 18%; four selectable FOV between 25 and 120mm
• Albira Si CT imaging module is based on a micro-focus X-ray source and a digital flat panel X-ray detector, 2400x2400 pixels. Typical performance characteristics: FOV-transaxial 70mm, axial 70/300 mm (single position/ moving table); Spatial resolution up to 90 microns; Minimum voxel size <35 microns; X-ray source energy range 10kV-50kV

 

 

OEC One Mobile C-arm Fluoroscopic X-ray System

The OEC One Mobile C-arm system uses X-rays to provide fluoroscopic and digital spot/film images of the subject anatomy, surgical tools/devices, and/or contrast agents. 

The maximum field of view is 9 inches and maximal output is 110kVp/20mA

 

FVMPE-RS Multiphoton Laser Scanning Microscope

test

 

 

X-Rad 320 Irradiator

The X-Rad 320 is a self-contained x-ray system designed to deliver a precise radiation dosage to biological samples including cell lines and rodents. The maximum output is 320kV with a maximum current of 30mA. The chamber contains an adjustable multi-position shelf with rotating platform to provide uniform radiation administration.

• kV settings: 5kV-320kV in 0.1kV increments
• mA settings: 0.5mA-30mA in 0.01mA increments
• Exposure timer: 1-99,999 seconds

 

Contacts

Name Role Phone Email Location
DLAR Imaging Core

 

 
DLARIC@umd.edu
 
6th floor A J Clark Hall
 
Beth Bauer
Imaging Core Director
 
301-405-6235
 
bbauer13@umd.edu
 
6106 A J Clark Hall
 
Larry Shelton
Attending Veterinarian
 
301-405-4920
 
ljsdvm@umd.edu
 
6110 A J Clark Hall
 
Betre Legesse
Technical Coordinator
 
301-405-5037
 
legesseb@umd.edu
 
6110 A J Clark Hall