iLab Organizer :: Vermont Biomedical Research Network (VBRN) Proteomics Facility

Overview of Services

The VBRN Proteomics Facility (https://vbrn.org/proteomics/) is located in the Firestone Medical Research Building (Room 143). It enables investigators to use an array of state-of-the-art mass spectrometry-based techniques for proteomics experiments, ranging from routine protein identification, post-translational modification characterization and finding protein interacting partners, to large-scale quantitative proteomics analyses using stable isotopes. Since its inception in 2006, the facility has analyzed over 20,000 samples and facilitated data acquisition to support close to 180 publications (https://vbrn.org/proteomics-publications/). The facility personnel train investigators in experimental design and proteomics methods, as well as assist with data interpretation, manuscript preparation, and grant submission. Our user base includes investigators from UVM and from institutions in 15 states. A summary of the facility’s impact on the development of the network can be found at https://vbrn.org/proteomics-impact/.

The Proteomics Facility (Room 143) is equipped with standard laboratory equipment (freezers, centrifuges, incubators, microscopes, and analytical balances, etc.), a Shimadzu analytical HPLC system with UV detection for offline fractionation, electrophoresis systems, a Sutter P-2000 laser puller, a Savant SpeedVac concentrator, a Labconco lyophilizer, five mass spectrometers from Thermo Fisher Scientific, as well as a hydrogen deuterium exchange (HDX) workflow station:

Oribtrap Eclipse mass spectrometer (MS) coupled to an EASY-nLC 1200 UPLC system
Orbitrap Exploris 240 MS Plus coupled to an EASY-nLC 1200 UPLC system
LTQ Orbitrap Discovery MS coupled to a Surveyor HPLC system
LTQ XL Linear Ion Trap MS equipped with electron transfer dissociation (ETD) coupled to a Surveyor HPLC system
LTQ Linear Ion Trap MS coupled to a Prominence (Shimadzu) HPLC system
HDX workflow station, HDx-3 PAL (Leap Technologies), which can be coupled to the LTQ or LTQ-Orbitrap Discovery MS with a Workplace Modular systems cart for conducting HDX MS experiments

The Data Processing unit (Room 143) maintains five computers (4 Dell Optiplex 7060 Workstations, i7 Processor, 32GB RAM, 2 displays for each, and 1 Dell Precision Tower 5810 with 1 display), and offers a suite of software including three copies of Proteome Discoverer embedded with SEQUEST, MASCOT and Byonic, SCAFFOLD Q+S, SIEVE, PEAKS, Skyline, SimGlycan, and HD Examiner to evaluate the datasets generated from various types of proteomics experiments.

The Proteomics Facility is located in the Firestone Medical Research Building and occupies 1,321 ft² of lab, data processing unit, office, and consultation space. There are bench spaces for accommodating up to six persons working simultaneously for sample preparation. The facility is supported by INBRE funding (P20GM103449).

Contacts

Name	Role	Phone	Email	Location
Dr. Bin Deng	Manager	(802) 656-5099	Bin.Deng@uvm.edu	FMRB 150
Dr. Ying Wai Lam	Core Director	(802) 656-4709	Ying-Wai.Lam@uvm.edu	FMRB 150
Sydney Cohn-Guthrie	Senior Lab Research Technician		Sydney.Cohn-Guthrie@uvm.edu	FMRB 150

Name

Role

Phone

Location

Dr. Bin Deng

Manager

(802) 656-5099

Bin.Deng@uvm.edu

FMRB 150

Dr. Ying Wai Lam

Core Director

(802) 656-4709

Ying-Wai.Lam@uvm.edu

FMRB 150

Sydney Cohn-Guthrie

Senior Lab Research Technician

Sydney.Cohn-Guthrie@uvm.edu

FMRB 150

Name	Description	Price
Consultation	First consult is free, each additional consult for a single project will be charged at the Consultation hourly rate.	Inquire
Consultation	Please consult with the facility personnel to: 1) select what sample preparation method(s) you would like to perform on your digests (e.g, Ziptip, TMT labeling, etc.) 2) select from the LC/MS category which instrument to use and the associated run time. A consultation is required prior to sample submission For gel samples, please provide an image of the gel and mark the bands or spots that are to be analyzed. We need to be sure your samples and sample preparation methods are compatible with mass spectrometry and your sample has an adequate amount of the target proteins to be measured by mass spectrometry. For protein samples in solution, you must provide us the expected protein concentration and the composition of the solution Turnaround time: 2-3 weeks	Inquire
De novo sequencing	A consultation is required prior to sample submission	Inquire
Desalting (for removing TMT labels)	Performed by facility personnel Desalting columns and reagents provided.	UVM Internal $58.24 each BPI $58.24 each Non-UVM $64.06 each Corporate $145.60 each
Desalting (for removing TMT labels)	Performed by user Desalting columns and reagents provided.	Corporate $58.40 each
Exact mass measurements (Chemistry)		UVM Internal $20.00 each
Exploris 240 (0.5 h) - simple mixture / Performed by facility personnel	Performed by facility personnel
Exploris 240 (0.5 h) - simple mixture / Performed by users	Performed by users
Exploris 240 (1 h) - complex mixture	Instrument only rate. Technical Assistance will be added where applicable based on each sample's specific requirements.	UVM Internal $127.50 each BPI $127.50 each Non-UVM $140.25 each Corporate $318.75 each
Exploris 240 (1 h) - complex mixture / Performed by facility personnel	Performed by facility personnel
Exploris 240 (3 h) - highly complex mixture / Performed by facility personnel	Performed by facility personnel
Exploris 240 (3 h) - highly complex mixture / Performed by users	Performed by users
Exploris 240 (0.25 h) - Education Samples	Class samples from UVM or BPIs Instrument only rate. Technical Assistance will be added where applicable based on each sample's specific requirements.	UVM Internal $63.75 each BPI $63.75 each Non-UVM $70.13 each Corporate $159.38 each
Exploris 240 (0.25 h) - Education Samples / performed by facility personnel	Class samples from UVM or BPIs Performed by facility personnel
Exploris 240 / Orbitrap Eclipse - Bulk (12 hrs/wk) - complex mixture / Performed by users	Self-independent hourly rate. Must use the Exploris 240 or Orbitrap Eclipse at least 12hrs a week to utilize the bulk discount rate.	UVM Internal $59.31 each BPI $59.31 each
Exploris 240 / Orbitrap Eclipse - Bulk (20 hrs/wk) - complex mixture / Performed by users	Self-independent hourly rate. Must use the Exploris 240 or Orbitrap Eclipse at least 20hrs a week to utilize the bulk discount rate.	UVM Internal $55.52 each BPI $55.52 each
Exploris 240 / Orbitrap Eclipse - Bulk (30 hrs/wk) - complex mixture / Performed by users	Self-independent hourly rate. Must use the Exploris 240 or Orbitrap Eclipse at least 30hrs a week to utilize the bulk discount rate.	UVM Internal $50.78 each BPI $50.78 each
Exploris 240 / Orbitrap Eclipse - Bulk (40 hrs/wk) - complex mixture / Performed by users	Self-independent hourly rate. Must use the Exploris 240 or Orbitrap Eclipse at least 40hrs a week to utilize the bulk discount rate.	UVM Internal $46.04 each BPI $46.04 each
Exploris 240 / Orbitrap Eclipse - Bulk (48 hrs/wk) - complex mixture / Performed by users	Self-independent hourly rate. Must use the Exploris 240 or Orbitrap Eclipse at least 48hrs a week to utilize the bulk discount rate.	UVM Internal $42.25 each BPI $42.25 each
High-pH fractionation / facility performed	Performed by facility personnel 1 set (8 fractions)	UVM Internal $171.06 each BPI $171.06 each Non-UVM $188.17 each Corporate $427.65 each
High-pH fractionation / user performed	Performed by users in the facility Kit and reagents provided. 1 set (8 fractions)	UVM Internal $39.69 each BPI $39.69 each Non-UVM $43.66 each Corporate $99.23 each
Identification of post-translational modifications (PTMs)	A consultation is required prior to sample submission	Inquire
Identification of protein-protein interacting partners (Interactomes)	A consultation is required prior to sample submission	Inquire
In-gel digestion	Performed by users in the facility Enzyme and reagents provided.	UVM Internal $15.87 each BPI $15.87 each Non-UVM $17.46 each Corporate $39.68 each
In-gel digestion (more than 15 gel bands)	Performed by facility personnel	UVM Internal $35.89 each BPI $35.89 each Non-UVM $39.48 each Corporate $89.73 each
In-gel digestion (Fewer than 15 gel bands)	Performed by facility personnel	UVM Internal $53.93 each BPI $53.93 each Non-UVM $59.32 each Corporate $134.83 each
In-solution digestion (User provides enzyme)	Performed by facility personnel	UVM Internal $104.72 each BPI $104.72 each Non-UVM $115.19 each Corporate $261.80 each
Label-Free quantification (Spectral counting)	A consultation is required prior to sample submission	Inquire
Lyophilizer	Operated by users (assisted by facility personnel, if necessary)	UVM Internal $25.98 each BPI $25.98 each Non-UVM $28.58 each Corporate $64.95 each
Orbitrap Eclipse (1 h) -complex mixture / Performed by facility personnel	Performed by facility personnel
Orbitrap Eclipse (3 h) - highly complex mixture / Performed by user	Performed by user
Orbitrap Eclipse MS (0.25 h) - Education Samples	Class samples from UVM or BPIs Instrument only rate. Technical Assistance will be added where applicable based on each sample's specific requirements.	UVM Internal $63.75 each BPI $63.75 each Non-UVM $70.13 each Corporate $159.38 each
Orbitrap Eclipse MS (0.25 h) - Education Samples / performed by facility personnel	Class samples from UVM or BPIs Performed by facility personnel
Orbitrap Eclipse MS (0.5 h) - simple mixture / Performed by facility personnel	Performed by facility personnel
Orbitrap Eclipse MS (0.5 h) - simple mixture / Performed by user	Performed by user
Orbitrap Eclipse MS (1 h) - complex mixture	Instrument only rate. Technical Assistance will be added where applicable based on each sample's specific requirements.	UVM Internal $127.50 each BPI $127.50 each Non-UVM $140.25 each Corporate $318.75 each
Orbitrap Eclipse MS (3 h) - highly complex mixture / performed by facility personnel	Performed by facility personnel
Peptide fractionation of complex mixtures	Off-line peptide separation (e.g., SCX) Strong cation exchange fractionation High-pH reverse phase fractionation A consultation is required prior to sample submission	Inquire
Phosphopeptide-enrichment/facility performed	Performed by facility personnel	UVM Internal $73.38 each BPI $73.38 each Non-UVM $80.72 each Corporate $183.45 each
Phosphopeptide-enrichment/user performed	Performed by users in the facility Kit and reagents provided.	UVM Internal $26.86 each BPI $26.86 each Non-UVM $29.55 each Corporate $67.15 each
Prepare Figures (fee for service)	per hour	UVM Internal $86.11 each BPI $86.11 each Non-UVM $94.72 each Corporate $215.28 each
Protein identification, isoform characterization (In-gel/In-solution digestion)	A consultation is required prior to sample submission	Inquire
Proteome Discoverer/Performed by facility personnel	Performed by facility personnel per hour	UVM Internal $86.11 each BPI $86.11 each Non-UVM $94.72 each Corporate $215.28 each
Proteome Discoverer/user performed	Performed by user	UVM Internal $0.00 each Non-UVM $0.00 each COM $0.00 each BPI $0.00 each
Quantification of target peptides using Parallel Reaction Monitoring and Skyline	A consultation is required prior to sample submission	Inquire
Scaffold/Performed by facility personnel	Performed by facility personnel per hour	UVM Internal $86.11 each BPI $86.11 each Non-UVM $94.72 each Corporate $215.28 each
Scaffold/user performed	Performed by user	UVM Internal $0.00 each Non-UVM $0.00 each COM $0.00 each BPI $0.00 each
Technical Assistance	hourly rate	Inquire
TMT labeling / facility performed (additional one set)	Performed by facility personnel Additional 1 set	UVM Internal $42.10 each BPI $42.10 each Non-UVM $46.31 each Corporate $105.25 each
TMT labeling / facility performed (one set)	Performed by facility personnel. 1 set (e.g., 10-plex)	UVM Internal $123.50 each BPI $123.50 each Non-UVM $135.85 each Corporate $308.75 each
TMT labeling / user performed	Performed by users in the facility TMT reagents NOT provided.	UVM Internal $18.84 each BPI $18.84 each Non-UVM $20.72 each Corporate $47.10 each
Zip tip / facility performed	Performed by facility personnel	UVM Internal $41.01 each BPI $41.01 each Non-UVM $45.11 each Corporate $102.53 each
Zip tip / user performed	Performed by users in the facility Kit and reagents provided.	UVM Internal $16.14 each BPI $16.14 each Non-UVM $17.75 each Corporate $40.35 each

▼ ► Consultation (1)
Name	Description	Price
Consultation	First consult is free, each additional consult for a single project will be charged at the Consultation hourly rate.	Inquire
▼ ► Data visualization and Figures preparation for publication (heat maps or volcano plots, etc.) (1)
Name	Description	Price
Prepare Figures (fee for service)	per hour	UVM Internal $86.11 each BPI $86.11 each Non-UVM $94.72 each Corporate $215.28 each
▼ ► Database searches / Software Analysis (with statistics) (4)
Name	Description	Price
Proteome Discoverer/Performed by facility personnel	Performed by facility personnel per hour	UVM Internal $86.11 each BPI $86.11 each Non-UVM $94.72 each Corporate $215.28 each
Proteome Discoverer/user performed	Performed by user	UVM Internal $0.00 each Non-UVM $0.00 each COM $0.00 each BPI $0.00 each
Scaffold/Performed by facility personnel	Performed by facility personnel per hour	UVM Internal $86.11 each BPI $86.11 each Non-UVM $94.72 each Corporate $215.28 each
Scaffold/user performed	Performed by user	UVM Internal $0.00 each Non-UVM $0.00 each COM $0.00 each BPI $0.00 each
▼ ► LC-MS (4)
Name	Description	Price
Exploris 240 (0.25 h) - Education Samples	Class samples from UVM or BPIs Instrument only rate. Technical Assistance will be added where applicable based on each sample's specific requirements.	UVM Internal $63.75 each BPI $63.75 each Non-UVM $70.13 each Corporate $159.38 each
Exploris 240 (1 h) - complex mixture	Instrument only rate. Technical Assistance will be added where applicable based on each sample's specific requirements.	UVM Internal $127.50 each BPI $127.50 each Non-UVM $140.25 each Corporate $318.75 each
Orbitrap Eclipse MS (0.25 h) - Education Samples	Class samples from UVM or BPIs Instrument only rate. Technical Assistance will be added where applicable based on each sample's specific requirements.	UVM Internal $63.75 each BPI $63.75 each Non-UVM $70.13 each Corporate $159.38 each
Orbitrap Eclipse MS (1 h) - complex mixture	Instrument only rate. Technical Assistance will be added where applicable based on each sample's specific requirements.	UVM Internal $127.50 each BPI $127.50 each Non-UVM $140.25 each Corporate $318.75 each
▼ ► Bulk LC-MS (5)
Name	Description	Price
Exploris 240 / Orbitrap Eclipse - Bulk (12 hrs/wk) - complex mixture / Performed by users	Self-independent hourly rate. Must use the Exploris 240 or Orbitrap Eclipse at least 12hrs a week to utilize the bulk discount rate.	UVM Internal $59.31 each BPI $59.31 each
Exploris 240 / Orbitrap Eclipse - Bulk (20 hrs/wk) - complex mixture / Performed by users	Self-independent hourly rate. Must use the Exploris 240 or Orbitrap Eclipse at least 20hrs a week to utilize the bulk discount rate.	UVM Internal $55.52 each BPI $55.52 each
Exploris 240 / Orbitrap Eclipse - Bulk (30 hrs/wk) - complex mixture / Performed by users	Self-independent hourly rate. Must use the Exploris 240 or Orbitrap Eclipse at least 30hrs a week to utilize the bulk discount rate.	UVM Internal $50.78 each BPI $50.78 each
Exploris 240 / Orbitrap Eclipse - Bulk (40 hrs/wk) - complex mixture / Performed by users	Self-independent hourly rate. Must use the Exploris 240 or Orbitrap Eclipse at least 40hrs a week to utilize the bulk discount rate.	UVM Internal $46.04 each BPI $46.04 each
Exploris 240 / Orbitrap Eclipse - Bulk (48 hrs/wk) - complex mixture / Performed by users	Self-independent hourly rate. Must use the Exploris 240 or Orbitrap Eclipse at least 48hrs a week to utilize the bulk discount rate.	UVM Internal $42.25 each BPI $42.25 each
▼ ► Sample Preparation (11)
Name	Description	Price
Desalting (for removing TMT labels)	Performed by facility personnel Desalting columns and reagents provided.	UVM Internal $58.24 each BPI $58.24 each Non-UVM $64.06 each Corporate $145.60 each
Desalting (for removing TMT labels)	Performed by user Desalting columns and reagents provided.	Corporate $58.40 each
In-gel digestion	Performed by users in the facility Enzyme and reagents provided.	UVM Internal $15.87 each BPI $15.87 each Non-UVM $17.46 each Corporate $39.68 each
In-gel digestion (more than 15 gel bands)	Performed by facility personnel	UVM Internal $35.89 each BPI $35.89 each Non-UVM $39.48 each Corporate $89.73 each
In-gel digestion (Fewer than 15 gel bands)	Performed by facility personnel	UVM Internal $53.93 each BPI $53.93 each Non-UVM $59.32 each Corporate $134.83 each
In-solution digestion (User provides enzyme)	Performed by facility personnel	UVM Internal $104.72 each BPI $104.72 each Non-UVM $115.19 each Corporate $261.80 each
Lyophilizer	Operated by users (assisted by facility personnel, if necessary)	UVM Internal $25.98 each BPI $25.98 each Non-UVM $28.58 each Corporate $64.95 each
Phosphopeptide-enrichment/facility performed	Performed by facility personnel	UVM Internal $73.38 each BPI $73.38 each Non-UVM $80.72 each Corporate $183.45 each
Phosphopeptide-enrichment/user performed	Performed by users in the facility Kit and reagents provided.	UVM Internal $26.86 each BPI $26.86 each Non-UVM $29.55 each Corporate $67.15 each
Zip tip / facility performed	Performed by facility personnel	UVM Internal $41.01 each BPI $41.01 each Non-UVM $45.11 each Corporate $102.53 each
Zip tip / user performed	Performed by users in the facility Kit and reagents provided.	UVM Internal $16.14 each BPI $16.14 each Non-UVM $17.75 each Corporate $40.35 each
▼ ► Tandem Mass Tags (5)
Name	Description	Price
High-pH fractionation / facility performed	Performed by facility personnel 1 set (8 fractions)	UVM Internal $171.06 each BPI $171.06 each Non-UVM $188.17 each Corporate $427.65 each
High-pH fractionation / user performed	Performed by users in the facility Kit and reagents provided. 1 set (8 fractions)	UVM Internal $39.69 each BPI $39.69 each Non-UVM $43.66 each Corporate $99.23 each
TMT labeling / facility performed (additional one set)	Performed by facility personnel Additional 1 set	UVM Internal $42.10 each BPI $42.10 each Non-UVM $46.31 each Corporate $105.25 each
TMT labeling / facility performed (one set)	Performed by facility personnel. 1 set (e.g., 10-plex)	UVM Internal $123.50 each BPI $123.50 each Non-UVM $135.85 each Corporate $308.75 each
TMT labeling / user performed	Performed by users in the facility TMT reagents NOT provided.	UVM Internal $18.84 each BPI $18.84 each Non-UVM $20.72 each Corporate $47.10 each
▼ ► Technical Assistance (1)
Name	Description	Price
Technical Assistance	hourly rate	Inquire
▼ ► Retired Services (21)
Name	Description	Price
Exact mass measurements (Chemistry)		UVM Internal $20.00 each
Exploris 240 (0.25 h) - Education Samples / performed by facility personnel	Class samples from UVM or BPIs Performed by facility personnel
Quantification of target peptides using Parallel Reaction Monitoring and Skyline	A consultation is required prior to sample submission	Inquire
Protein identification, isoform characterization (In-gel/In-solution digestion)	A consultation is required prior to sample submission	Inquire
Exploris 240 (0.5 h) - simple mixture / Performed by facility personnel	Performed by facility personnel
Identification of protein-protein interacting partners (Interactomes)	A consultation is required prior to sample submission	Inquire
Exploris 240 (0.5 h) - simple mixture / Performed by users	Performed by users
Identification of post-translational modifications (PTMs)	A consultation is required prior to sample submission	Inquire
De novo sequencing	A consultation is required prior to sample submission	Inquire
Exploris 240 (1 h) - complex mixture / Performed by facility personnel	Performed by facility personnel
Peptide fractionation of complex mixtures	Off-line peptide separation (e.g., SCX) Strong cation exchange fractionation High-pH reverse phase fractionation A consultation is required prior to sample submission	Inquire
Exploris 240 (3 h) - highly complex mixture / Performed by facility personnel	Performed by facility personnel
Exploris 240 (3 h) - highly complex mixture / Performed by users	Performed by users
Label-Free quantification (Spectral counting)	A consultation is required prior to sample submission	Inquire
Orbitrap Eclipse MS (0.25 h) - Education Samples / performed by facility personnel	Class samples from UVM or BPIs Performed by facility personnel
Consultation	Please consult with the facility personnel to: 1) select what sample preparation method(s) you would like to perform on your digests (e.g, Ziptip, TMT labeling, etc.) 2) select from the LC/MS category which instrument to use and the associated run time. A consultation is required prior to sample submission For gel samples, please provide an image of the gel and mark the bands or spots that are to be analyzed. We need to be sure your samples and sample preparation methods are compatible with mass spectrometry and your sample has an adequate amount of the target proteins to be measured by mass spectrometry. For protein samples in solution, you must provide us the expected protein concentration and the composition of the solution Turnaround time: 2-3 weeks	Inquire
Orbitrap Eclipse MS (0.5 h) - simple mixture / Performed by facility personnel	Performed by facility personnel
Orbitrap Eclipse MS (0.5 h) - simple mixture / Performed by user	Performed by user
Orbitrap Eclipse (1 h) -complex mixture / Performed by facility personnel	Performed by facility personnel
Orbitrap Eclipse MS (3 h) - highly complex mixture / performed by facility personnel	Performed by facility personnel
Orbitrap Eclipse (3 h) - highly complex mixture / Performed by user	Performed by user

▼ ► Consultation (1)

▼ ► Data visualization and Figures preparation for publication (heat maps or volcano plots, etc.) (1)

▼ ► Database searches / Software Analysis (with statistics) (4)

▼ ► LC-MS (4)

▼ ► Bulk LC-MS (5)

▼ ► Sample Preparation (11)

Name

Description

Price

Desalting (for removing TMT labels)

Performed by facility personnel

Desalting columns and reagents provided.

UVM Internal $58.24 each
BPI $58.24 each
Non-UVM $64.06 each
Corporate $145.60 each

Desalting (for removing TMT labels)

Performed by user

Desalting columns and reagents provided.

Corporate $58.40 each

In-gel digestion

Performed by users in the facility

Enzyme and reagents provided.

UVM Internal $15.87 each
BPI $15.87 each
Non-UVM $17.46 each
Corporate $39.68 each

In-gel digestion (more than 15 gel bands)

Performed by facility personnel

UVM Internal $35.89 each
BPI $35.89 each
Non-UVM $39.48 each
Corporate $89.73 each

In-gel digestion (Fewer than 15 gel bands)

Performed by facility personnel

UVM Internal $53.93 each
BPI $53.93 each
Non-UVM $59.32 each
Corporate $134.83 each

In-solution digestion (User provides enzyme)

Performed by facility personnel

UVM Internal $104.72 each
BPI $104.72 each
Non-UVM $115.19 each
Corporate $261.80 each

Lyophilizer

Operated by users (assisted by facility personnel, if necessary)

UVM Internal $25.98 each
BPI $25.98 each
Non-UVM $28.58 each
Corporate $64.95 each

Phosphopeptide-enrichment/facility performed

Performed by facility personnel

UVM Internal $73.38 each
BPI $73.38 each
Non-UVM $80.72 each
Corporate $183.45 each

Phosphopeptide-enrichment/user performed

Performed by users in the facility

Kit and reagents provided.

UVM Internal $26.86 each
BPI $26.86 each
Non-UVM $29.55 each
Corporate $67.15 each

Zip tip / facility performed

Performed by facility personnel

UVM Internal $41.01 each
BPI $41.01 each
Non-UVM $45.11 each
Corporate $102.53 each

Zip tip / user performed

Performed by users in the facility

Kit and reagents provided.

UVM Internal $16.14 each
BPI $16.14 each
Non-UVM $17.75 each
Corporate $40.35 each

▼ ► Tandem Mass Tags (5)

▼ ► Technical Assistance (1)

▼ ► Retired Services (21)

Name

Description

Price

Exact mass measurements (Chemistry)

UVM Internal $20.00 each

Exploris 240 (0.25 h) - Education Samples / performed by facility personnel

Class samples from UVM or BPIs

Performed by facility personnel

Quantification of target peptides using Parallel Reaction Monitoring and Skyline

A consultation is required prior to sample submission

Inquire

Protein identification, isoform characterization (In-gel/In-solution digestion)

A consultation is required prior to sample submission

Inquire

Exploris 240 (0.5 h) - simple mixture / Performed by facility personnel

Performed by facility personnel

Identification of protein-protein interacting partners (Interactomes)

A consultation is required prior to sample submission

Inquire

Exploris 240 (0.5 h) - simple mixture / Performed by users

Performed by users

Identification of post-translational modifications (PTMs)

A consultation is required prior to sample submission

Inquire

De novo sequencing

A consultation is required prior to sample submission

Inquire

Exploris 240 (1 h) - complex mixture / Performed by facility personnel

Performed by facility personnel

Peptide fractionation of complex mixtures

Off-line peptide separation (e.g., SCX)
Strong cation exchange fractionation
High-pH reverse phase fractionation

A consultation is required prior to sample submission

Inquire

Exploris 240 (3 h) - highly complex mixture / Performed by facility personnel

Performed by facility personnel

Exploris 240 (3 h) - highly complex mixture / Performed by users

Performed by users

Label-Free quantification (Spectral counting)

A consultation is required prior to sample submission

Inquire

Orbitrap Eclipse MS (0.25 h) - Education Samples / performed by facility personnel

Class samples from UVM or BPIs

Performed by facility personnel

Consultation

Please consult with the facility personnel to:

1) select what sample preparation method(s) you would like to perform on your digests (e.g, Ziptip, TMT labeling, etc.)

2) select from the LC/MS category which instrument to use and the associated run time.

A consultation is required prior to sample submission

For gel samples, please provide an image of the gel and mark the bands or spots that are to be analyzed. We need to be sure your samples and sample preparation methods are compatible with mass spectrometry and your sample has an adequate amount of the target proteins to be measured by mass spectrometry.
For protein samples in solution, you must provide us the expected protein concentration and the composition of the solution

Turnaround time: 2-3 weeks

Inquire

Orbitrap Eclipse MS (0.5 h) - simple mixture / Performed by facility personnel

Performed by facility personnel

Orbitrap Eclipse MS (0.5 h) - simple mixture / Performed by user

Performed by user

Orbitrap Eclipse (1 h) -complex mixture / Performed by facility personnel

Performed by facility personnel

Orbitrap Eclipse MS (3 h) - highly complex mixture / performed by facility personnel

Performed by facility personnel

Orbitrap Eclipse (3 h) - highly complex mixture / Performed by user

Performed by user

Vermont Biomedical Research Network (VBRN) Proteomics Facility

Location and Hours

Getting Started

Links and Resources

Operational Polices

Overview of Services

Contacts

Contacts