Absolute quantification of proteins using stable isotopically labeled standards
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A consultation is required prior to sample submission
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Inquire
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Consultation
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Please consult with the facility personnel to:
1) select what sample preparation method(s) you would like to perform on your digests (e.g, Ziptip, TMT labeling, etc.)
2) select from the LC/MS category which instrument to use and the associated run time.
A consultation is required prior to sample submission
- For gel samples, please provide an image of the gel and mark the bands or spots that are to be analyzed. We need to be sure your samples and sample preparation methods are compatible with mass spectrometry and your sample has an adequate amount of the target proteins to be measured by mass spectrometry.
- For protein samples in solution, you must provide us the expected protein concentration and the composition of the solution
Turnaround time: 2-3 weeks
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Inquire
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De novo sequencing
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A consultation is required prior to sample submission
|
Inquire
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Desalting (for removing TMT labels)
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Performed by facility personnel
Desalting columns and reagents provided.
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UVM Internal
$39.55
each
External
$43.51
each
COM
$29.66
each
BPI
$39.55
each
|
Exploris 240 (0.5 h) - simple mixture / Performed by facility personnel
|
Performed by facility personnel
|
UVM Internal
$89.02
each
External
$97.92
each
COM
$66.77
each
BPI
$89.02
each
|
Exploris 240 (0.5 h) - simple mixture / Performed by users
|
Performed by users
|
UVM Internal
$75.46
each
External
$83.01
each
COM
$56.60
each
BPI
$75.46
each
|
Exploris 240 (1 h) - complex mixture / Performed by facility personnel
|
Performed by facility personnel
|
UVM Internal
$112.72
each
External
$101.49
each
COM
$84.54
each
BPI
$112.72
each
|
Exploris 240 (1 h) - complex mixture / Performed by users
|
Performed by users
|
UVM Internal
$100.46
each
External
$110.51
each
COM
$75.35
each
BPI
$100.46
each
|
Exploris 240 (3 h) - highly complex mixture / Performed by facility personnel
|
Performed by facility personnel
|
UVM Internal
$213.31
each
External
$234.64
each
COM
$159.98
each
BPI
$213.31
each
|
Exploris 240 (3 h) - highly complex mixture / Performed by users
|
Performed by users
|
UVM Internal
$200.46
each
External
$220.51
each
COM
$150.35
each
BPI
$200.46
each
|
Exploris 240 (0.25 h) - Education Samples / performed by facility personnel
|
Class samples from UVM or BPIs
Performed by facility personnel
|
UVM Internal
$69.64
each
External
$76.60
each
COM
$52.23
each
BPI
$69.64
each
|
Exploris 240 (0.25 h) - Education Samples / Performed by users
|
Class samples from UVM or BPIs
Performed by users
|
UVM Internal
$55.46
each
External
$61.01
each
COM
$37.50
each
BPI
$55.46
each
|
High-pH fractionation / facility performed
|
Performed by facility personnel
1 set (8 fractions)
|
UVM Internal
$110.87
each
External
$121.96
each
COM
$83.15
each
BPI
$110.87
each
|
High-pH fractionation / user performed
|
Performed by users in the facility
Kit and reagents provided.
1 set (8 fractions)
|
UVM Internal
$30.41
each
External
$33.45
each
COM
$22.81
each
BPI
$30.41
each
|
Identification of post-translational modifications (PTMs)
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A consultation is required prior to sample submission
|
Inquire
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Identification of protein-protein interacting partners (Interactomes)
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A consultation is required prior to sample submission
|
Inquire
|
In-gel digestion
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Performed by users in the facility
Enzyme and reagents provided.
|
UVM Internal
$15.34
each
External
$16.87
each
COM
$11.50
each
BPI
$15.34
each
|
In-gel digestion (more than 15 gel bands)
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Performed by facility personnel
|
UVM Internal
$34.14
each
External
$37.55
each
COM
$25.60
each
BPI
$34.14
each
|
In-gel digestion (Fewer than 15 gel bands)
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Performed by facility personnel
|
UVM Internal
$37.09
each
External
$40.80
each
COM
$27.82
each
BPI
$37.09
each
|
In-solution digestion (User provides enzyme)
|
Performed by facility personnel
|
UVM Internal
$67.57
each
External
$74.33
each
COM
$50.68
each
BPI
$67.57
each
|
Label-Free quantification (Spectral counting)
|
A consultation is required prior to sample submission
|
Inquire
|
Lyophilizer
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Operated by users (assisted by facility personnel, if necessary)
|
UVM Internal
$53.43
each
External
$58.77
each
COM
$43.43
each
BPI
$53.43
each
|
Orbitrap Eclipse (1 h) -complex mixture / Performed by facility personnel
|
Performed by facility personnel
|
UVM Internal
$107.63
each
External
$118.39
each
COM
$80.72
each
BPI
$107.63
each
|
Orbitrap Eclipse (3 h) - highly complex mixture / Performed by user
|
Performed by user
|
UVM Internal
$200.46
each
External
$220.51
each
COM
$150.35
each
BPI
$200.46
each
|
Orbitrap Eclipse MS (0.25 h) - Education Samples / performed by facility personnel
|
Class samples from UVM or BPIs
Performed by facility personnel
|
UVM Internal
$69.02
each
External
$75.92
each
COM
$51.77
each
BPI
$69.02
each
|
Orbitrap Eclipse MS (0.25 h) - Education Samples / performed by users
|
Class samples from UVM or BPIs
Education Samples / Performed by users
|
UVM Internal
$55.46
each
External
$61.01
each
COM
$37.50
each
BPI
$55.46
each
|
Orbitrap Eclipse MS (0.5 h) - simple mixture / Performed by facility personnel
|
Performed by facility personnel
|
UVM Internal
$89.02
each
External
$97.92
each
COM
$66.77
each
BPI
$89.02
each
|
Orbitrap Eclipse MS (0.5 h) - simple mixture / Performed by user
|
Performed by user
|
UVM Internal
$75.46
each
External
$83.01
each
COM
$56.60
each
BPI
$75.46
each
|
Orbitrap Eclipse MS (1 h) - complex mixture / Performed by user
|
Performed by user
|
UVM Internal
$100.46
each
External
$110.51
each
COM
$75.35
each
BPI
$100.46
each
|
Orbitrap Eclipse MS (3 h) - highly complex mixture / performed by facility personnel
|
Performed by facility personnel
|
UVM Internal
$210.16
each
External
$231.18
each
COM
$157.62
each
BPI
$210.16
each
|
Peptide fractionation of complex mixtures
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- Off-line peptide separation (e.g., SCX)
- Strong cation exchange fractionation
- High-pH reverse phase fractionation
A consultation is required prior to sample submission
|
Inquire
|
Phosphopeptide-enrichment/facility performed
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Performed by facility personnel
|
UVM Internal
$50.19
each
External
$55.21
each
COM
$37.64
each
BPI
$50.19
each
|
Phosphopeptide-enrichment/user performed
|
Performed by users in the facility
Kit and reagents provided.
|
UVM Internal
$20.51
each
External
$22.56
each
COM
$15.38
each
BPI
$22.26
each
|
Prepare Figures (fee for service)
|
fee / figure
|
UVM Internal
$469.84
each
External
$516.83
each
COM
$352.38
each
BPI
$469.84
each
|
Protein identification, isoform characterization (In-gel/In-solution digestion)
|
A consultation is required prior to sample submission
|
Inquire
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Proteome Discoverer/Performed by facility personnel
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Performed by facility personnel
Fee for all samples submitted in 1 batch
|
UVM Internal
$58.37
each
External
$64.20
each
COM
$43.77
each
BPI
$58.37
each
|
Proteome Discoverer/user performed
|
Performed by user
|
UVM Internal
$0.00
each
External
$0.00
each
COM
$0.00
each
BPI
$0.00
each
|
Quantification of target peptides using Parallel Reaction Monitoring and Skyline
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A consultation is required prior to sample submission
|
Inquire
|
Quantitative Proteomics (with peptide fractionation/enrichment)
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Mass spectrometry-based global quantification of protein expression and PTMs in biological samples using stable isotopes.
- Stable Isotope Labeling by Amino acids in Cell culture (SILAC)
- Dimethyl Labeling
- Tandem Mass Tags
|
Inquire
|
Scaffold/Performed by facility personnel
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Performed by facility personnel
All samples submitted at 1 time
|
UVM Internal
$53.22
each
External
$58.54
each
COM
$39.92
each
BPI
$53.22
each
|
Scaffold/user performed
|
Performed by user
|
UVM Internal
$0.00
each
External
$0.00
each
COM
$0.00
each
BPI
$0.00
each
|
TMT labeling / facility performed (additional one set)
|
Performed by facility personnel
Additional 1 set
|
UVM Internal
$27.72
each
External
$30.49
each
COM
$20.79
each
BPI
$27.72
each
|
TMT labeling / facility performed (one set)
|
Performed by facility personnel.
1 set (e.g., 10-plex)
|
UVM Internal
$79.66
each
External
$87.63
each
COM
$59.75
each
BPI
$79.66
each
|
TMT labeling / user performed
|
Performed by users in the facility
TMT reagents NOT provided.
|
UVM Internal
$12.88
each
External
$14.17
each
COM
$9.66
each
BPI
$12.88
each
|
Zip tip / facility performed
|
Performed by facility personnel
|
UVM Internal
$29.40
each
External
$32.34
each
COM
$22.05
each
BPI
$29.40
each
|
Zip tip / user performed
|
Performed by users in the facility
Kit and reagents provided.
|
UVM Internal
$11.31
each
External
$12.44
each
COM
$8.48
each
BPI
$11.31
each
|