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Vermont Biomedical Research Network (VBRN) Proteomics Facility

 

 

Location and Hours

Hours

Monday - Friday

9 AM - 5 PM 

 

Location

University of Vermont

Marsh Life Science Building

Rooms 335 and 337

Getting Started

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Links and Resources

  1. VBRN Proteomics Core Website
  2. Facility Supported Publications

Operational Polices

 

Overview of Services

The VBRN Proteomics Facility (https://vbrn.org/proteomics/) is located in the Marsh Life Science Building (Room 333 - 337).  It enables investigators to use an array of state-of-the-art mass spectrometry-based techniques for proteomics experiments, ranging from routine protein identification, post-translational modification characterization and finding protein interacting partners, to large-scale quantitative proteomics analyses using stable isotopes. Since its inception in 2006, the facility has analyzed over 20,000 samples and facilitated data acquisition to support close to 180 publications (https://vbrn.org/proteomics-publications/). The facility personnel train investigators in experimental design and proteomics methods, as well as assist with data interpretation, manuscript preparation, and grant submission.  Our user base includes investigators from UVM and from institutions in 15 states.  A summary of the facility’s impact on the development of the network can be found at https://vbrn.org/proteomics-impact/.

 

The Proteomics Facility (Room 335)  is equipped with standard laboratory equipment (freezers, centrifuges, incubators, microscopes, and analytical balances, etc.), a Shimadzu analytical HPLC system with UV detection for offline fractionation, electrophoresis systems, a Sutter P-2000 laser puller, a Savant SpeedVac concentrator, a Labconco lyophilizer, five mass spectrometers from Thermo Fisher Scientific, as well as a hydrogen deuterium exchange (HDX) workflow station:

 

  • Orbitrap Fusion Tribrid mass spectrometer (MS) coupled to an EASY-nLC ULTRA UPLC system
  • Q-Exactive Hybrid Quadrupole-Orbitrap MS Plus coupled to an EASY-nLC 1200 UPLC system
  • LTQ Orbitrap Discovery MS coupled to a Surveyor HPLC system
  • LTQ XL Linear Ion Trap MS equipped with electron transfer dissociation (ETD) coupled to a Surveyor HPLC system
  • LTQ Linear Ion Trap MS coupled to a Prominence (Shimadzu) HPLC system
  • HDX workflow station, HDx-3 PAL (Leap Technologies), which can be coupled to the LTQ or LTQ-Orbitrap Discovery MS with a Workplace Modular systems cart for conducting HDX MS experiments

 

The Data Processing unit (Room 333) maintains five computers (4 Dell Optiplex 7060 Workstations, i7 Processor, 32GB RAM, 2 displays for each, and 1 Dell Precision Tower 5810 with 1 display), and offers a suite of software including three copies of Proteome Discoverer embedded with SEQUEST, MASCOT and Byonic, SCAFFOLD Q+S, SIEVE, PEAKS, Skyline, SimGlycan, and HD Examiner to evaluate the datasets generated from various types of proteomics experiments.

 The Proteomics Facility is located in the Marsh Life Science Building and occupies 1,408 ft2 of lab, data processing unit, office, and consultation space.  There are bench spaces for accommodating up to six persons working simultaneously for sample preparation. The facility is supported by INBRE funding (P20GM103449).



Contacts 

 

Dr. Ying Wai Lam

Core Director
(802) 656-4709
Ying-Wai.Lam@uvm.edu

Dr. Bin Deng

Manager
(802) 656-5099
Bin.Deng@uvm.edu

Clarissa Gold

Lab Research Technician
Clarissa.Gold@uvm.edu

Contacts

Name Role Phone Email Location
Dr. Bin Deng
Manager
 
(802) 656-5099
 
Bin.Deng@uvm.edu
 
337 Marsh Life Science Building
 
Dr. Ying Wai Lam
Core Director
 
(802) 656-4709
 
Ying-Wai.Lam@uvm.edu
 
337 Marsh Life Science Building
 

Price List


Search available services: View: by category alphabetically
Current Proteomics Facility Capabilities (8)
Data visualization and Figures preparation for publication (heat maps or volcano plots, etc.) (1)
Database searches / Software Analysis (with statistics) (4)
LC-MS (24)
Sample Preparation (10)
Tandem Mass Tags (5)