Welcome to the Parnassus Flow Cytometry CoLab (PFCC) iLab page. Please read a brief overview regarding the Core below. For more information visit the PFCC website.
If you are a UCSF or UCSF-affiliated user please Sign In above to view the Scheduler and Services information.
Overview of Services
The PFCC is a shared resource laboratory which oversees flow and mass cytometry instruments at the Parnassus Campus and another UCSF campus sites. The Core exists to provide Diabetes Research Center (DRC) members and fellow UCSF researchers valuable resources in the field of flow and mass cytometry and operates under the following directives:
Provide assistance and exceptional customer service to researchers along with acquisition, setup, maintenance, and support of instrumentation.
Teach users the information they need to know as well as provide them with the opportunity to learn more details should they request it.
Monitor research and development opportunities, experiment with novel techniques, and test new equipment.
Consolidate and bring UCSF flow cytometry groups together and standardize practices.
How to Get Started? Visit the New Users page on our website to get informed.
All Onboarded users of PFCC
While on Schedule Instrument tab navigate to the Training category and schedule the appropriate class for your training. Visit our website HERE for details on each class.
All Trained users of PFCC
While onSchedule Instrument tab navigate to the respective Instrument Category and click [View Schedule] to reserve it as needed.
For information about PFCC Facilities Policies visit the PFCC websitefor details on:
Hands-on training with a staff member for FACSAria SuperUser training. Training is charged by the hour plus the hourly rate of the instrument.
Length: approximately 1 hr per day (scheduled at least 4 days during Core's morning QC window 8-10AM)
Please keep in mind:
Only frequent FACSAria users can sign up for Advanced Sorter training
Recommended for new users to gain experience of sorting during business hours for at least 1-2 months before signing up for Advanced Training
Only one Hands-on Training request accomodated per week; you will be placed on waiting list if next week's class already assigned.
Please allow at least 48hrs response time from the Team to confirm your request, if needed please email flowtraining@ucsf.edu
Inquire
Assistance (on instrument)
Operator Assistance with a staff member. The Operator Assistance service is requesting a Flow Core staff member to be at a specific instrument to run your samples for you. Assistance can also be requested if you need instrument setup on weekends, holidays, and non-normal work hours (Monday-Friday from 6pm-8am at standard rate).
Class 1 - Principles of Flow Cytometry (Set by Staff)
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Class 2 - FACSDiva (Set by Staff)
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Class 3 - Cell Sorting (Set by Staff)
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CyTOF Helios (Charmander / Blue)
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FACSAria (II / IIu / Fusion)
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Fortessa ( Cookie, / Bert / Ernie / Grover)
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Guidance Fee
Hands on Guidance with a staff member up to 30min. The Guidance service is requesting a Flow Core staff member to help you become more familiar with the instrument after the initial training. Following training, Flow Core staff will guide you while running your first few experiments, or if you want us to look over your instrument settings. Please book an instrument first and request Guidance 1-week in advance by emailing flowassist@ucsf.edu.
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LSRII (Oscar / Kermit / BigBird)
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MIBIscope (Littlefoot)
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Nanosight NS300 (Skipper)
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Nanosight Training, Hour
Hands-on training with a staff member to introduce NanoSight NS300 "Skipper". Training is charged by the hour plus the hourly rate of the instrument.
Length: approximately 2 hr
Please keep in mind:
All user must first attend the "Onboarding Session" before signing up for any Hands-on Session
Only one Hands-on session accomodated per week
Please email flowtraining@ucsf.edu, allow at least 48hrs response time from the Team to confirm your request.
Inquire
Onboarding Session (Set by Staff)
Add event to Every New User
Sessions are always on Mondays 10-11 am in room S854A; if there is a holiday, session will be on the following day (Tuesday)
Inquire
Operator Assistance
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Plate Reader Training, Hour
Hand-on training with a staff member to introduce operation of Plate Reader and/or advance tools on the analyzers. Training is charged by the hour plus the hourly rate of the instrument.
Length: approximately 1 hr
Please keep in mind:
Only trained and competent Analyzer users can sign up for this Advanced training.
Recommended for new users to gain experience during business hours for at least 1-2 months before signing up for Advanced Training.
Only one Hands-on Training request accomodated per week; you will be placed on waiting list if next week's session already assigned.
Please allow email Flowtraining@ucsf.edu or Flowassist@ucsf.edu for availability, Allow at least 48hrs response time from the Team.
Inquire
Sony SH800 (Crash Bandicoot)
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Supplies for Conjugations
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UCSF Academic FCS Express Site License
For more information about UCSF’s FCS Express site license please visit our website under the Services / Software section.
Operator Assistance with a staff member. The Operator Assistance service is requesting a Flow Core staff member to be at a specific instrument to run your samples for you. Assistance can also be requested if you need instrument setup on weekends, holidays, and non-normal work hours (Monday-Friday from 6pm-8am at standard rate).
Hand-on training with a staff member to introduce operation of Plate Reader and/or advance tools on the analyzers. Training is charged by the hour plus the hourly rate of the instrument.
Length: approximately 1 hr
Please keep in mind:
Only trained and competent Analyzer users can sign up for this Advanced training.
Recommended for new users to gain experience during business hours for at least 1-2 months before signing up for Advanced Training.
Only one Hands-on Training request accomodated per week; you will be placed on waiting list if next week's session already assigned.
Please allow email Flowtraining@ucsf.edu or Flowassist@ucsf.edu for availability, Allow at least 48hrs response time from the Team.
Inquire
Nanosight Training, Hour
Hands-on training with a staff member to introduce NanoSight NS300 "Skipper". Training is charged by the hour plus the hourly rate of the instrument.
Length: approximately 2 hr
Please keep in mind:
All user must first attend the "Onboarding Session" before signing up for any Hands-on Session
Only one Hands-on session accomodated per week
Please email flowtraining@ucsf.edu, allow at least 48hrs response time from the Team to confirm your request.
Inquire
Advanced Sorter Training - FACSAria, Hour
Hands-on training with a staff member for FACSAria SuperUser training. Training is charged by the hour plus the hourly rate of the instrument.
Length: approximately 1 hr per day (scheduled at least 4 days during Core's morning QC window 8-10AM)
Please keep in mind:
Only frequent FACSAria users can sign up for Advanced Sorter training
Recommended for new users to gain experience of sorting during business hours for at least 1-2 months before signing up for Advanced Training
Only one Hands-on Training request accomodated per week; you will be placed on waiting list if next week's class already assigned.
Please allow at least 48hrs response time from the Team to confirm your request, if needed please email flowtraining@ucsf.edu
Inquire
Onboarding Session (Set by Staff)
Add event to Every New User
Sessions are always on Mondays 10-11 am in room S854A; if there is a holiday, session will be on the following day (Tuesday)
Inquire
Class 1 - Principles of Flow Cytometry (Set by Staff)
Inquire
Class 2 - FACSDiva (Set by Staff)
Inquire
Class 3 - Cell Sorting (Set by Staff)
Inquire
Guidance Fee
Hands on Guidance with a staff member up to 30min. The Guidance service is requesting a Flow Core staff member to help you become more familiar with the instrument after the initial training. Following training, Flow Core staff will guide you while running your first few experiments, or if you want us to look over your instrument settings. Please book an instrument first and request Guidance 1-week in advance by emailing flowassist@ucsf.edu.
Welcome!
