Harry Hood Bassett Flow Cytometry and Small Particles Detection (FCSPD) Facility

Hello FLOW Users,

To help ensure a smooth transition to the new MIC iLab account, we’ll be keeping the FLOW iLab core open through Friday, October 31st. This gives us a bit more time to support users as they get set up on the MIC system. 

If you're a new FLOW user, please reach out to Amy Gilman (Amy.Gilman@med.uvm.edu) for onboarding support with both FLOW and MIC, including equipment use and training requirements.

Starting November 1^st, please, begin using Cytometry equipment calendars and requesting services through the new Microscopy Imaging and Cytometry iLab page: https://uvm.ilab.agilent.com/service_center/show_external/3159

If you run into any issues or have questions, feel free to contact me at Nicole.DeLance@med.uvm.edu or Amy Gilman.

Thank you for your patience and understanding as we traverse these new waters together.

Overview of Services

The Harry Hood Bassett Flow Cytometry and Small Particle Detection (FCSPD) facility at UVM is a College of Medicine Core Facility designed as a multi-user resource for the high speed analysis, sorting of cells, and the characterization of small particles. The FCSPD facility has two convenitonal analytical cytometers (Miltenyi MACSQuant VYB, 3L/8 fluorescences and Beckman CytoFlex, 2L/4 fluorescences), a full spectra flow cytometer (Cytek Aurora, 4L/54 fluorescent channels), and a BD FACSAriaIII high-speed cell sorter (4L/13 fluorescences). In addition, the facility houses the Particle Metrix ZetaView Twin nanoparticle tracker analyzer (2L/2 fluorescences). This equipment was purchased through National Institute of Health and UVM-College of Medicine Shared Instrumentation Grants. Investigators who bring samples to the FCSPD facility for sorting or analysis pay a fee, which is used to offset operating expenses. The FCSPD facility provides professional consultation and assistance with equipment use, experimental design, and interpretation of data.

Leadership

Dr. Douglas Taatjes - Facility Director

In an ongoing effort to balance rigor and reproducibility as we strive to provide excellent customer service to all of our clients, if you experience any service issues please email Douglas.Taatjes@med.uvm.edu directly

iLab Assistance or Card Access:

Please contact Amy.Gilman@med.uvm.edu or Nicole.DeLance@med.uvm.edu for card access to FMRB 185A for sample submission and use of VIGR.

Location and hours of operation

Links and Resources

1. Flow Cytometry and Cell Sorting (FCCS) Facility

Contacts