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Marshall University Genomic and Bioinformatics Core

Overview of Services

The MU Genomics Core Facility provides Next Generation Sequencing (NGS) and Sanger sequencing services to investigators at universities, and in government and industry. The Genomics Core is equipped with an Illumina HiSeq1500 sequencer with the capability to perform whole genome sequencing, whole exome sequencing, transcriptome expression profiling (RNA-Seq) and microbiome studies. The HiSeq1500 can generate 1.5 billion single reads/run or 3 billion paired reads/run. In a 2 x100 paired-end run, ~300 Gigabases can be sequenced.

Investigators must meet with Core Personnel prior to any experimentation or sample preparation. At the initial meeting, Genomics Core staff and the investigator will define experimental design and objectives, technical issues (e.g. DNA/RNA quality and quantity, and library construction), probable sequence yields and outcomes, and overall cost.

Investigators must provide input DNA or RNA which meets minimum quality and quantity requirements. The Genomics Core will prepare standard Illumina sequencing libraries (whole genome, whole exome, ChIP-Seq and RNA-Seq) based on established protocols. Libraries may also be prepared by the investigator. The Core will generate clusters and sequence the libraries according to the experimental design and perform data analyses as requested by the investigator (e.g. variant calling and transcriptome analysis).

 

iLab Solutions

New Users: To register for an iLab account 

1. Click here to login and register

2. Complete & submit the registration form. If you are not a PI, you will need to enter your PI’s first and last name.

3. iLab will send you a welcome email with login credentials. You should receive the Welcome Email from iLab with these credentials, typically within one business day 

 

Leadership

Dr. Don Primerano
Genomics Core Director
Phone: 304-696-7338
Email: primeran@marshall.edu

Location and hours of operation

Hours Location

 

Monday - Friday

8 AM - 5 PM 


Byrd Biotech Center 

1700 3rd Avenue

Huntington, WV 25701

Links and Resources of Marshall University Core

  1. Genomics Core Facility Website

  2. Marshall University Bioinformatics

  3. Biospecimen-Processing-Core

  4. Sanger DNA Sequencing Service

  5. Shared Instruments

 

**Please remember to cite or acknowledge the Genomics Core, Bioinformatics Core, the WV-INBRE grant (P20GM103434), the COBRE ACCORD grant (1P20GM121299) and the West Virginia Clinical and Translational Science Institute (WV-CTSI) grant (2U54GM104942) in relevant publications.**

Contacts

Name Role Phone Email Location
Don Primerano, Ph.D.
Core Director
 
304-696-7338
 
primeran@marshall.edu
 

 
Jim Denvir, Ph.D.
Associate Professor, Bioinfomaticist
 
304-696-7327
 
denvir@marshall.edu
 
BBSC Room 336L
 
Jun Fan, Ph.D.
Genomics Core Manager
 
304-696-7344
 
fanj@marshall.edu
 
BBSC Room 336U
 
Robin Turner, MS
Research Technician II
 
304-696-3720
 
roberts2@marshall.edu
 
BBSC Room 336T
 
Alejandro Nato, Ph.D.
Assistant Professor, Bioinformaticist
 
304-696-3562
 
nato@marshall.edu
 
BBSC Room 336M
 

Map


https://goo.gl/maps/dSRdWtPjYk22