Cell Science Core

Our Lab:

The Cell Science Core (CSC) specializes in protein and small molecules analysis, cell analysis, and cell culture techniques. Services include:  2-D gel electrophoresis, 2-D DIGE, LC-MS/MS, HPLC, flow cytometry, cell and tissue culture, and immortalization of cell lines. Our staff works closely with investigators to help design, perform, and analyze experiments. Additionally, the CSC staff also offer training and assistance in flow cytometry, tissue culture, and operation many of our walk-up instruments.


The CSC is a component of the Center for Pediatric Research at the Nemours/Alfred I. duPont Hospital for Children located in Wilmington, Delaware. We are supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P30GM114736. Any publication of data generated using the CSC services or instruments should cite the grant number (see citation).

Instruments:

  • C6 Flow Cytometer (Accuri/BD Biosciences)
  • Novocyte 3000 Flow Cytometer (ACEA Biosciences)
  • FSC Express (DeNovo) - software for flow cytometry data analysis
  • 6460 Triple Quadrupole LC-MS/MS (Agilent)
  • Typhoon Trio Scanner (GE Lifesciences)
  • Centro XS Luminometer (Berthold)
  • Hemavet 950 Blood Analyzer (Drew Scientific)

Services:

  • 2-D gel electrophoresis/2D-DIGE
  • LC-MS/MS analysis of compounds  
  • Cell immortilization

Citation:

 -please cite our grants in any publication of data-

The Cell Science Core is supported by the NIH COBRE program, and the NIH requires all investigators (faculty, staff, or students) who publish/present  data funded wholly or in part by COBRE funds – including use of cores – to acknowledge the grant through citation. Contact us if you have any questions.

  • All publications, articles, abstracts, and presentation using data from the instrument must include one of the following statements:
    • This project was supported by the COBRE program, with a grant from the National Institute of General Medical Sciences - NIGMS (P30 GM114736) from the National Institutes of Health.
    • Short citation for abstracts: Supported by a grant from NIH-NIGMS (P30 GM114736).
  • In addition, NIH also requires this disclaimer:
    • This content is solely the responsibility of the authors and does not necessarily represent the official views of NIH.

Leadership

Robert W. Mason, PhD

Core Director

(302) 651.6885

robert.mason@nemours.org

Location

1701 Rockland Road

Rockland Center One, 2nd Floor

Wilmington, DE 19803

Links and Resources

  1. Center for Pediatric Research (at Nemours/Alfred I. duPont Hospital for Children)
  2. Centralized Research Instrumentation Core (CRIC) (Delaware INBRE)
  3. Core Facilities Searchable Database (at UD and affiliated institutions)

Contacts

Name Role Phone Email Location
Lisa Glazewski, MS
Core Manager/Flow Cytometry Specialist
 
(302) 651-6707
 
CellScienceCore@Nemours.org
 
Rockland Center One, rm 221
 
Stephanie Tantzer, MS
LC-MS/MS Specialist
 
(302) 651-6707
 
Stephanie.Tantzer@Nemours.org
 
Rockland Center One, rm 221
 

Map



Members at The Delaware Vermont iLab Network (DEVIN)

Delaware State University

Core Name Primary Contact Email Phone Number/Ext
Oscar Imaging Facility Michael Moore mmoore@desu.edu 302-857-7035

Nemours

Core Name Primary Contact Email Phone Number/Ext
Cell Science Core Lisa Glazewski, MS CellScienceCore@Nemours.org (302) 651-6707
HTS and Drug Discovery Sigrid Langhans langhans@nemoursresearch.org (302) 651-6538
Healthcare Delivery Science Jennifer Christofferson jennifer.christofferson@nemours.org 302-298-7876
Histochemistry and Tissue Processing Core Histochemistry and Tissue Processing Core 1600 Rockland Road, Wilmington DE 19803
Translational Neuroimaging Core Shaohui (Shao) Zhang, PhD Shaohui.Zhang@nemours.org 302-651-6869
Biomolecular Core Laboratory Jennifer Holbrook mbcore@nemours.org 302-651-6824 Fax: 302-651-6767

University of Delaware

Core Name Primary Contact Email Phone Number/Ext
University of Delaware DNA Sequencing & Genotyping Center

Bruce Kingham brucek@udel.edu (302) 831-0823
Core Name Primary Contact Email Phone Number/Ext
Bioinformatics Core

Dr. Bryan Ballif bryan.ballif@uvm.edu (802) 656-1389
Proteomics Facility

Dr. Ying Wai Lam Ying-Wai.Lam@uvm.edu (802) 656-4709