Cell Science Core

Our Lab:

The Cell Science Core (CSC) specializes in protein and small molecules analysis, cell analysis, and cell culture techniques. Services include:  2-D gel electrophoresis, 2-D DIGE, LC-MS/MS, HPLC, flow cytometry, cell and tissue culture, and immortalization of cell lines. Our staff works closely with investigators to help design, perform, and analyze experiments. Additionally, the CSC staff also offer training and assistance in flow cytometry, tissue culture, and operation many of our walk-up instruments.


The CSC is a component of the Center for Pediatric Research at the Nemours/Alfred I. duPont Hospital for Children located in Wilmington, Delaware. We are supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P30GM114736. Any publication of data generated using the CSC services or instruments should cite the grant number (see citation).

Instruments:

  • FACS Aria III (BD Biosciences)
  • C6 Flow Cytometer (Accuri/BD Biosciences)
  • Novocyte 3000 Flow Cytometer (ACEA Biosciences)
  • FSC Express (DeNovo) - software for flow cytometry data analysis
  • 6460 Triple Quadrupole LC-MS/MS (Agilent)
  • Typhoon Trio Scanner (GE Lifesciences)
  • Centro XS Luminometer (Berthold)
  • Hemavet 950 Blood Analyzer (Drew Scientific)
  • Victor Nivo 5F Plate Reader (Perkin Elmer)

Services:

  • 2-D gel electrophoresis/2D-DIGE
  • LC-MS/MS analysis of compounds  
  • Cell immortilization

Citation:

 -please cite our grants in any publication of data-

The Cell Science Core is supported by the NIH COBRE program, and the NIH requires all investigators (faculty, staff, or students) who publish/present  data funded wholly or in part by COBRE funds – including use of cores – to acknowledge the grant through citation. Contact us if you have any questions.

  • All publications, articles, abstracts, and presentation using data from the instrument must include one of the following statements:
    • This project was supported by the COBRE program, with a grant from the National Institute of General Medical Sciences - NIGMS (P30 GM114736) from the National Institutes of Health.
    • Short citation for abstracts: Supported by a grant from NIH-NIGMS (P30 GM114736).
  • In addition, NIH also requires this disclaimer:
    • This content is solely the responsibility of the authors and does not necessarily represent the official views of NIH.

Leadership

Robert W. Mason, PhD

Core Director

(302) 651.6885

robert.mason@nemours.org

Location

e400, room 535

200 Powder Mill Rd.

Wilmington, DE 19803

 

Contacts

Name Role Phone Email Location
Lisa Glazewski, MS
Core Lab Manager
 
(302) 651-4892
 
Lisa.Glazewski@Nemours.org
 
e400, room 535
 
Stephanie Tantzer, MS
LC-MS/MS Specialist
 

 
Stephanie.Tantzer@Nemours.org
 
Rockland Center One, rm 221
 

Map


https://goo.gl/maps/mYzR1P2NYj52


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