Cell Science Core

Our Core Lab:

The Cell Science Core (CSC) specializes in cell, protein, and small molecules analysis as well as cell culture techniques. Services include:  2-D gel electrophoresis, 2-D DIGE, LC-MS/MS, HPLC, flow cytometry, fluorescence-activated cell sorting (FACS), cell and tissue culture, and immortalization of cell lines. Our staff works closely with investigators to help design, perform, and analyze experiments. Additionally, the CSC staff offers training and assistance in flow cytometry, tissue culture, and operation many of our walk-up instruments.

NIH Support and Citation:

We are supported by the an Institutional Development Award (IDeA) under grant number P30GM114736, and the NIH requires all investigators (faculty, staff, or students) who publish/present data funded wholly or in part by these funds – including use of cores – to acknowledge the grant through citation. Contact us if you have any questions.

  • All publications, articles, abstracts, or presentations using data from the instruments/services must include one of the following statements:
    • This project was supported by the COBRE program, with a grant from the National Institute of General Medical Sciences - NIGMS (P30 GM114736) from the National Institutes of Health.
    • Short citation for abstracts: Supported by a grant from NIH-NIGMS (P30 GM114736).
  • In addition, NIH also requires this disclaimer:
    • This content is solely the responsibility of the authors and does not necessarily represent the official views of NIH.

Instruments:

  • Cell Sorter: FACS Aria III, BD Biosciences
  • Flow Cytometers, analyzers:
    • C6, Accuri/BD Biosciences
    • Novocyte 3000, ACEA Biosciences
    • software for analysis: FSC Express, DeNovo software
  • LC-MS/MS: 6460 Triple Quadrupole, Agilent
  • Typhoon Trio Scanner, GE Lifesciences
  • Blood Analyzer: Hemavet 950, Drew Scientific
  • Plate Readers:
    • Victor Nivo 5F, Perkin Elmer
    • Luminometer: Centro XS, Berthold

Services:

  • Cell Sorting (FACS)
  • 2-D gel electrophoresis/2D-DIGE
  • LC-MS/MS analysis of compounds  
  • Cell immortilization

Leadership

Sonali Barwe, PhD

Core Director

(302) 651.6542

sonali.barwe@nemours.org

Location

The CSC is a component of the Center for Pediatric Research at the Nemours/Alfred I. duPont Hospital for Children in Wilmington, Delaware. Our labs are located on the Dupont Experimental Station Campus in rooms 5431, 5435, and 5443 of Building 400.

Building 400, 5th floor
200 Powder Mill Rd.
Wilmington, DE 19803

Contacts

Name Role Phone Email Location
Lisa Glazewski, MS
Core Lab Manager
 
(302) 651-4892
 
Lisa.Glazewski@Nemours.org
 
e400
 
Sonali Barwe, PhD
Core Lab Director
 
(302) 651-6542
 
sonali.barwe@nemours.org
 
e400
 

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