Arrayed ORF Screens
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- For custom arrayed screens, inquire with the core
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Inquire
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Arrayed shRNA Screens
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- For custom arrayed screens, inquire with the core
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Inquire
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Concentrated viral stock
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- At least 50 μl at a minimum titer of 108 TU/ml, as determined on HEK 293 cells; in DMEM + 5% serum
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Inquire
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Concentrated viral stock (prepared from customer-supplied plasmid)
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- At least 50 μl at a minimum titer of 108 TU/ml, as determined on HEK 293 cells; in DMEM + 5% serum
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Inquire
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CRISPR control cell line
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- Inducible Cas9 + sgRNA that targets the AAV integration site
- Cas9 expressed from pCW-Cas9 (Tet-ON, puromycin resistance)
- sgRNA expressed from pLX-sgRNA (U6 promoter, blasticidin resistance)
- An additional optimization charge of $150 per parental cell line may apply
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Inquire
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EnVision Multilabel Plate Reader
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- Fluorescence itnsity, fluorescence polarization, time-resolved fluorescence, luminescence, absorbance, alphaLISA
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Inquire
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Gene activation (CRISPRa)
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- Inducible dCas9 fused to VP16 tetramer activation domain (VP64) + sgRNA
- Inactive dCas-VP64 fusion expressed from pHAGE TRE dCas9-VP64 (D10A, H840A mutant; Tet-ON, neomycin resistance)
- sgRNA expressed from pLX-sgRNA (U6 promoter, blasticidin resistance)
- Use of multiple sgRNAs may increase level of activation (additional charge applies)
- An additional optimization charge of $150 per parental cell line may apply
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Inquire
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Gene knockin
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- Inducible Cas9 + sgRNA; customer must provide homology template containing sequence to be knocked in and appropriate homology arms
- Cas9 expressed from pCW-Cas9 (Tet-ON, puromycin resistance)
- sgRNA expressed from pLX-sgRNA (U6 promoter, blasticidin resistance)
- An additional optimization charge of $150 per parental cell line may apply
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Inquire
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Gene knockout
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- Inducible Cas9 + sgRNA
- Cas9 expressed from pCW-Cas9 (Tet-ON, puromycin resistance)
- sgRNA expressed from pLX-sgRNA (U6 promoter, blasticidin resistance)
- An additional optimization charge of $150 per parental cell line may apply.
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Inquire
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Generation of polyclonal Cas9 cell line
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- Transduction with Cas9 lentivirus and selection on antibiotic
- Wild-type Cas9 (for knockouts, deletions, mutations, and knockins) or dCas9-VP64 (for activation)
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Inquire
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Generation of polyclonal Cas9/sgRNA cell line
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- sgRNA design and cloning
- Preparation of Cas9 and sgRNA lentivirus
- Transduction with Cas9 lentivirus and selection on antibiotic
- Wild-type Cas9 (for knockouts, deletions, mutations, and knockins) or dCas9-VP64 (for activation)
- Transduction with sgRNA lentivirus and selection on blasticidin
- Engineered cells delivered as frozen stocks
- Customer must induce Cas9 expression with doxycycline to effect gene editing or activation
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Inquire
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Generation of polyclonal Cas9/sgRNA cell line from Cas9 cell line
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- sgRNA design and cloning
- Preparation and titering of sgRNA lentivirus
- Transduction with sgRNA lentivirus and selection on blasticidin
- Engineered cells delivered as frozen stocks
- Customer must induce Cas9 expression with doxycycline to effect gene editing or activation
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Inquire
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Homology repair template design
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- Design of template for mutation or knockin experiment
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Inquire
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Lentiviral supernatant
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- At least 200 μl at a minimum titer of 106 TU/ml, as determined on HEK 293T cells; in DMEM + 5% serum
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Inquire
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Lentiviral supernatant (prepared from customer-supplied plasmid)
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- Prepared from customer-supplied plasmid (1 ug plasmid DNA required)
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Inquire
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Operetta High-Content Imaging System
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- 96-well, 384-well, and other formats
- Fluorescence and brightfield imaging
- Confocal imaging option
- Four excitation and emission filters
- Environmental control chamber for live-cell imaging
- Image analysis software with web-based access
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Inquire
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Plasmid DNA
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- At least 1 μg of plasmid.
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Inquire
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Pooled CRISPR screen - large screen
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- Generation of pooled lentiviral library
- Generation of Cas9 cell line
- Preparation of samples for next generation sequencing
- Customer must perform the cell work and apply the selection
- Sequencing cost is extra
- Applies to screens with large libraries, difficult-to-transduce cells, or large numbers of samples, where viral prep must be scaled up significantly
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Inquire
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Pooled CRISPR screen - standard screen
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- Generation of pooled lentiviral library
- Generation of Cas9 cell line
- Preparation of samples for next generation sequencing
- Customer must perform the cell work and apply the selection
- Sequencing cost is extra
- Extra charge may be incurred for very large libraries, difficult-to-transduce cells, or large sample number
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Inquire
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Pooled CRISPR Screens
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- Pooled CRISPR screens: Lentiviral CRISPR libraries
- Does not include sequencing cost
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Inquire
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Pooled shRNA Screens
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- Pooled shRNA screens: Lentiviral shRNA libraries (human & mouse;pRsII2 vector; U6 promoter, RFP, puromycin resistance)
- Does not include sequencing cost
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Inquire
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Production of sgRNA lentivirus
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- sgRNA design and cloning
- Preparation and titering of sgRNA lentivirus
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Inquire
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shRNA- or ORF-expressing cell line
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- Includes virus preparation, transduction, and selection
- An additional optimization charge of $100 per parental cell line may apply
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Inquire
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