Flow Cytometry

 

Flow Cytometry Center Of Research Excellence

Our mission is to provide all members of the ISMMS community with outstanding research cytometry Service, to disseminate technical knowledge through Education, and to advance scientific discovery through Innovation. The medium of our mission is flow cytometry.
 

The ability to measure high-order multidimensional data with single cell granularity is the scientific power of flow cytometry. To acquire these measurements at rates of thousands per second and virtually unlimited population size is the statistical glory of flow cytometry. To use this data and return a highly purified subpopulation of live functional cells is the magic of cell sorting.
 

Given this power, the CORE provides all members of the Mount Sinai community the instrumentation and subject expertise to incorporate flow cytometry and cell sorting into any research project. Please explore these web pages to learn more about our amazing services and brilliant people.

 

 Access and Training

 

New users of the Flow Cytometry CORE iLabs site can download the User Guides in the section below titled " "links and resources"

The CORE operates a structured training program that is detailed on the Request Services page. The sequence of classes begins with the Introduction to the CORE. 

 

 About Acknowledgments

 

The lifeblood of an academic institution is publication. Likewise the metric of success of a shared resource facility is acknowledgement. Please remember to acknowledge the contribution of the CORE to your publications. We suggest including in your acknowledgements section verbiage such as:

"We would like to thank the expertise and assistance of the Dean's Flow Cytometry CORE at Mount Sinai."

Authorship, however, should follow the guidelines set forth in the Faculty Handbook.

 

Current Rates

The current recharge rates for each machine can be downloaded in this PDF document.

Location and hours of operation

 

Hours	Location	Other Dean’s COREs at Mount Sinai
Hours available: 24/7 Hours staffed: 10:00AM - 5:00PM	Core Operations Manager: Icahn School of Medicine 1425 Madison Avenue Floor 13 , room 13-05  Phone: : 212-659-9370 New York, New York 10029 Cytometer Locations: Icahn Building IMI 13-05 Analyzer room Icahn Building IMI 13-52a BSL2 Sorter room Icahn Building IMI 13-03 Sorter room Hess Building CSM S5-304 BSL2 Sorter room 787 11th Ave 600H Analyzers Annenberg Building 14-17 Analyzer	Biorepository and Pathology Core Irradiator CoRE Facility Microscopy CoRE Mouse Genetics Neuropathology Brain Bank & Research Core Stem Cell Engineering Core

Almost real-time Instrument status please click here. 

Links and Resources

1. Fluorochrome tools:
1. Easy Panel
2. Fluorescent Protein database
2. iLabs Guide for Flow Users
3. iLabs Guide for PI's and Department administrators
4. CORE SOP index
5. Useful References:
• Flow Cytometry - First Principles by Alice Longobardi Givan (click download button and add yourself to the BC marketing list)
• Flow Cytometry - A Basic Introduction by Michael Ormerod
• Current Protocols in Cytometry
6. Societies and Communities:
• International Society for Advancement of Cytometry
• Purdue Cytometry List

      8- Tutorials.

• BD learning
• Everything Aurora and ImageStream
• Thermo Flow cytometry

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ATTENTION! If your fund number is missing from your lab, go to the "Membership Requests & Funds" tab, scroll down to the "Request access to additional funds" field, and enter the Cloud Number. If the Cloud Number doesn't appear, please email the Sinai IT Team (Nilesh.Ainchwar@mountsinai.org, Lane.Blumberg@mountsinai.org, Sandeep.KumarVerma@mountsinai.org, Sugandha.Miglani@mountsinai.org, Ayan.Saha@mountsinai.org, Vikas.Telukunta@mountsinai.org, iLab@mssm.edu) to request that the Cloud Number be imported to iLab. If your Cloud Number can't be imported, you'll be referred to SPF@mountsinai.org to get your Cloud Number renewed/extended.
If the Cloud Number is in the lab but you can't make a reservation or purchase, please check with the PI and/or Lab Finance Manager, as they can grant you access to a fund number in the lab. If you can access the Cloud Number but still can't reserve equipment, check with the Core and ensure that training isn't needed to make a reservation. If it's not a training requirement, please email ilab-support@agilent.com for technical support.

Note: iLab pulls Cloud (Fund) Number details from the COA Website (http://coa.mountsinai.org), and if the expiration date shows as expired, iLab purges it from the system.

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Contacts

Name	Role	Phone	Email	Location
Jordi Ochando	Scientific Director	212-824-8430	jordi.ochando@mssm.edu	Hess S5-113
Xuqiang Qiao	Core Scientist		xuqiang.qiao@mssm.edu	Hess 5 S5-304
Guillermo Villegas	Core Operations Manager	212-659-9370	guillermo.villegas@mssm.edu	Icahn 13
Alessandro Marins Dos Santos	Senior Core Scientist	212-659-9370	alessandro.marinsdossantos@mssm.edu	Icahn 13
Edgardo Aritzia	Senior Core Scientist		edgardo.aritzia@mssm.edu	Hess 6
Jovvian Parakkal	Senior Core Scientist	212-659-9370	jovvian.parakkal@mssm.edu	Icahn 13
Martina Di Verniere	Senior Research Associate	212-659-9370	martina.diverniere@mssm.edu	Icahn 13

Available Equipment and Resources

▼ ► Analyzer (7)

Name Price 787 Fortessa View calendar A14 Aurora View calendar Attune View calendar I13 Aurora View calendar I13 Canto II View calendar ImageStream View calendar Symphony A5 SE View calendar

▼ ► Retired (1)

Name Price CSM5L View calendar

▼ ► Sorter (4)

Name Price AuroraCS View calendar Tyto View calendar Default additional charges Tyto Cartridge Inquire IMI4L View calendar CytoFLEX SRT View calendar