xx MDACC Biomolecular Structure and Function Core (DISABLED)

Overview of Services

The purpose of the Biomolecular Structure and Function Core is to provide recombinant protein production, protein characterization, binding studies and structural biology (X-ray crystallography) services to MD Anderson Cancer Center and the wider Texas Medical Center.

Protein Production Services

Plasmid expression vector cloning

suite of E. coli expression vectors available (various affinity tag options)

E. coli test expression

screening for optimal strain/media combination for soluble protein production

Large scale protein purification

multiple liter scale production

X-ray Crystallography Services

Crystallization screening

960 condition crystallization trial
automated imaging of crystallization trial
visible and UV imaging for protein crystal detection

Crystallization optimization

customized screening around 'crystallization hits'
crystal growth seeding

X-ray diffraction data collection

data collection at high intensity synchrotron beamline (Advanced photon source)

X-ray structure determination

Protein Characterization Services

Differential scanning fluorimetry (DSF)

measures protein melting temperature, T_m (thermal stability)
compare stability of wild type vs mutant protein
buffer screening for protein stabilization
compound screening (T_m shift)

Size exclusion chromatography with static light scattering (SEC-MALS)

chromatography separation of protein species with MW determination by light scattering for each species.
autosampler allows multiple sample setup

Analytical ultracentrifugation (AUC)

direct determination or protein MW in solution
K_d for protein self-association or complex formation
assess distribution protein oligomeric states
compare wild type vs mutant proteins
up to 7x samples per experiment run

Binding Studies Services

Isothermal titration calorimetry (ITC)

measure binding affinity, stoichiometry, enthalpy, entropy and free energy
protein-ligand, protein-peptide, protein-DNA or protein-protein interactions
label free technique (no labeling artifacts)
autosampler - allows multiple sample setup

Biolayer interferometry (Octet)

measure binding affinity & on-rate/off-rate for an interaction
protein-ligand, protein-peptide, protein-DNA or protein-protein interactions
compound screening
96 or 384 well format

Surface Plasmon Resonance (SensiQ)
- measure binding affinity & on-rate/off-rate for an interaction
- protein-ligand, protein-peptide, protein-DNA or protein-protein interactions
- compound screening (including fragment compounds)

Microscale thermophoresis (MST)

measure binding affinity
protein-ligand, protein-peptide, Protein-DNA or protein-protein interactions
low volume (10 µL sample per concentration point)
detects change in MW and/or hydration shell surface of the protein upon ligand binding.

Fluorescence anisotropy (FA)

measure affinity of complex formation
protein-ligand, protein-peptide, Protein-DNA interactions.
compound screening
96 or 384 well format

Leadership

Phil Jones

Head of Drug Discovery, Institute for Applied Cancer Science

Biomolecular Structure and Function Core Oversight

PJones3@mdanderson.org

Core Staff

Name	Role	Location	Phone	email
Paul Leonard	Director	3SCR3 3442	832-750-1516	PLeonard1@mdanderson.org
Sarah Joseph	Senior Research Assistant	3SCR3 4410	713-745-4700	SJoseph14@mdanderson.org

Location and hours of operation

Hours	Location
Monday - Friday 9 AM - 5 PM	South Campus Research Building 3, 1881 East Road, Houston, TX 77054

Contacts

Name	Role	Phone	Email	Location
Paul Leonard	Director	832-750-1516	PLeonard1@mdanderson.org	3SCR3 3442

Price List

Name	Description	Price
12L scale protein production	12 Liter E. coli culture with recombinant protein expression of your desired protein Purification using affinity chromatography, size exclusion chromatography and ion exchange as required.	Inquire
2L scale protein production	2 liter E. coli culture growth with recombinant protein expression of your desired protein Purification using affinity chromatography, size exclusion chromatography and ion exchange chromatography as required.	Inquire
2L scale Test Purification	2 liter E. coli culture growth and test purification (Ni-NTA, SEC and ion exchange if required)	Inquire
6L scale protein production	6 liter E. coli culture growth with recombinant protein expression of your desired protein Purification using affinity chromatography, size exclusion chromatography and ion exchange chromatography as required.	Inquire
Analytical ultracentrifugation experiment (per run - up to 7x samples)	Experiment design and setup Instrument usage/maintenance Data analysis and reporting	Inquire
Budget E. coli expression testing (13-48 plasmids)	one test condition per plasmid construct E.coli strain: Rosetta2 pLysS, SoluBL21, C41 Media: auto-induction ZYP5052 Expression temperature: 18°C	Inquire
Budget E. coli expression testing (49-88 plasmids)	one test condition per plasmid construct E.coli strain: Rosetta2 pLysS, SoluBL21, C41 Media: auto-induction ZYP5052 Expression temperature: 18°C	Inquire
Differential scanning fluorimetry (DSF) - for 1 sample performed in triplicate	Experiment setup Data collection Data analysis and reporting	Inquire
Fine screen and optimization of initial hits		Inquire
Independent Instrument Use Daily Fee (Biomek FX liquid handler)	Independent Instrument Use Daily Fee Beckman Biomek Fx system for 96 well or 384 well dispensing	Inquire
Independent Instrument Use Daily Fee (ITC, MST, Octet & DSF)	Isothermal titration calorimetry (TA instruments affinity ITC system) Microscale thermophoresis (Nanotemper) Octet/Biolayer interferometry (Pal Forte Bio) Differential scanning fluorimetry	Inquire
Initial Crystallography Screening	Crystallization trial of 960 crystallization trial conditions at 3x protein concentrations	Inquire
Isothermal titration calorimetry (ITC) - per experiment	label free binding studies (no labeling artifacts) determine Kd, stoichiometry, and thermodynamics (enthalpy, entropy and free energy) Experiment Definition: One titration of ligand into protein AND a titration of the ligand into buffer as the heat of dilution control experiment.	Inquire
Microscale Thermophoresis (MST) - experiment design and sample preparation	Experiment planning and design Protein labeling with a fluorophore Purification of labeled protein from free dye Testing capillary compatibility	Inquire
Microscale Thermophoresis (MST) - per experiment	Experiment definition: Record thermophoresis data at 3x MST powers (30%, 60% and 90%) Experiment setup Instrument use and consumables Data fitting and reporting	Inquire
Octet (Biolayer interferometry) - experiment design and sample preparation	Experiment planning and design Protein labeling with a biotin Purification of labeled protein from free biotin	Inquire
Octet (Biolayer interferometry) - per ligand (experiment)	Octet (Biolayer interferometry) - per ligand (experiment)	Inquire
Octet biochip (per chip)	Pal ForteBio Octet Biochip	Inquire
Plasmid cloning (E. coli expression vector) - 25-48 plasmids	Choice of E. coli expression vector N-terminal tag options: His₆-TEVsite, His₆-MBP-TEVsite, His₆-GST-TEVsite, His₆-SUMO-TEVsite, His₆-StrepII-TEVsite, C-terminal tag options: TEVsite-His₆	Inquire
Plasmid cloning (E. coli expression vector) - 41-80 plasmids	Choice of E. coli expression vector N-terminal tag options: His₆-TEVsite, His₆-MBP-TEVsite, His₆-GST-TEVsite, His₆-SUMO-TEVsite, His₆-StrepII-TEVsite, C-terminal tag options: TEVsite-His₆	Inquire
Plasmid cloning (E. coli expression vector) - 6-24 plasmids	Choice of E. coli expression vector N-terminal tag options: His₆-TEVsite, His₆-MBP-TEVsite, His₆-GST-TEVsite, His₆-SUMO-TEVsite, His₆-StrepII-TEVsite, C-terminal tag options: TEVsite-His₆	Inquire
Plasmid cloning - per plasmid	Choice of E. coli expression vector N-terminal tag options: His₆-TEVsite, His₆-MBP-TEVsite, His₆-GST-TEVsite, His₆-SUMO-TEVsite, His₆-StrepII-TEVsite, C-terminal tag options: TEVsite-His₆	Inquire
Premium E. coli expression testing (13-48 plasmids)	Matrix of 16 tests per plasmid construct 4x E.coli strains: BL21 (DE3), Rosetta2 pLysS, SoluBL21, C41 4x media options: Terrific broth, Superior, Power and auto-induction ZYP5052 Expression temperature: 18°C	Inquire
Premium E. coli expression testing (49-88 plasmids)	Matrix of 16 tests per plasmid construct 4x E.coli strains: BL21 (DE3), Rosetta2 pLysS, SoluBL21, C41 4x media options: Terrific broth, Superior, Power and auto-induction ZYP5052 Expression temperature: 18°C	Inquire
Premium E. coli expression testing (per plasmid)	Matrix of 16 tests per plasmid construct 4x E.coli strains: BL21 (DE3), Rosetta2 pLysS, SoluBL21, C41 4x media options: Terrific broth, Superior, Power and auto-induction ZYP5052 Expression temperature: 18°C	Inquire
SEC-MALS (experiment setup and 1 sample test)	Separates protein species by size using size exclusion chromatography Wyatt multi-angle light scattering system determines MW of each protein species autosampler - allows multiple sample setup	Inquire
SEC-MALS - each additional sample with data report	Separates protein species by size using size exclusion chromatography Wyatt multi-angle light scattering system determines MW of each protein species autosampler - allows multiple sample setup	Inquire
Structure determination		Inquire
Synchrotron data collection	Synchrotron data collection (per hour)	Inquire
Training Fee (ITC, MST, Octet or DSF)		Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Cloning (4)
Plasmid cloning - per plasmid	Choice of E. coli expression vector N-terminal tag options: His₆-TEVsite, His₆-MBP-TEVsite, His₆-GST-TEVsite, His₆-SUMO-TEVsite, His₆-StrepII-TEVsite, C-terminal tag options: TEVsite-His₆	Inquire
Plasmid cloning (E. coli expression vector) - 6-24 plasmids	Choice of E. coli expression vector N-terminal tag options: His₆-TEVsite, His₆-MBP-TEVsite, His₆-GST-TEVsite, His₆-SUMO-TEVsite, His₆-StrepII-TEVsite, C-terminal tag options: TEVsite-His₆	Inquire
Plasmid cloning (E. coli expression vector) - 25-48 plasmids	Choice of E. coli expression vector N-terminal tag options: His₆-TEVsite, His₆-MBP-TEVsite, His₆-GST-TEVsite, His₆-SUMO-TEVsite, His₆-StrepII-TEVsite, C-terminal tag options: TEVsite-His₆	Inquire
Plasmid cloning (E. coli expression vector) - 41-80 plasmids	Choice of E. coli expression vector N-terminal tag options: His₆-TEVsite, His₆-MBP-TEVsite, His₆-GST-TEVsite, His₆-SUMO-TEVsite, His₆-StrepII-TEVsite, C-terminal tag options: TEVsite-His₆	Inquire
▼ ► E. coli test expression (5)
Name	Description	Price
Premium E. coli expression testing (per plasmid)	Matrix of 16 tests per plasmid construct 4x E.coli strains: BL21 (DE3), Rosetta2 pLysS, SoluBL21, C41 4x media options: Terrific broth, Superior, Power and auto-induction ZYP5052 Expression temperature: 18°C	Inquire
Budget E. coli expression testing (13-48 plasmids)	one test condition per plasmid construct E.coli strain: Rosetta2 pLysS, SoluBL21, C41 Media: auto-induction ZYP5052 Expression temperature: 18°C	Inquire
Premium E. coli expression testing (13-48 plasmids)	Matrix of 16 tests per plasmid construct 4x E.coli strains: BL21 (DE3), Rosetta2 pLysS, SoluBL21, C41 4x media options: Terrific broth, Superior, Power and auto-induction ZYP5052 Expression temperature: 18°C	Inquire
Budget E. coli expression testing (49-88 plasmids)	one test condition per plasmid construct E.coli strain: Rosetta2 pLysS, SoluBL21, C41 Media: auto-induction ZYP5052 Expression temperature: 18°C	Inquire
Premium E. coli expression testing (49-88 plasmids)	Matrix of 16 tests per plasmid construct 4x E.coli strains: BL21 (DE3), Rosetta2 pLysS, SoluBL21, C41 4x media options: Terrific broth, Superior, Power and auto-induction ZYP5052 Expression temperature: 18°C	Inquire
▼ ► Protein Production (4)
Name	Description	Price
2L scale Test Purification	2 liter E. coli culture growth and test purification (Ni-NTA, SEC and ion exchange if required)	Inquire
2L scale protein production	2 liter E. coli culture growth with recombinant protein expression of your desired protein Purification using affinity chromatography, size exclusion chromatography and ion exchange chromatography as required.	Inquire
6L scale protein production	6 liter E. coli culture growth with recombinant protein expression of your desired protein Purification using affinity chromatography, size exclusion chromatography and ion exchange chromatography as required.	Inquire
12L scale protein production	12 Liter E. coli culture with recombinant protein expression of your desired protein Purification using affinity chromatography, size exclusion chromatography and ion exchange as required.	Inquire
▼ ► X-ray crystallography (4)
Name	Description	Price
Initial Crystallography Screening	Crystallization trial of 960 crystallization trial conditions at 3x protein concentrations	Inquire
Fine screen and optimization of initial hits		Inquire
Synchrotron data collection	Synchrotron data collection (per hour)	Inquire
Structure determination		Inquire
▼ ► Protein characterization (4)
Name	Description	Price
SEC-MALS (experiment setup and 1 sample test)	Separates protein species by size using size exclusion chromatography Wyatt multi-angle light scattering system determines MW of each protein species autosampler - allows multiple sample setup	Inquire
SEC-MALS - each additional sample with data report	Separates protein species by size using size exclusion chromatography Wyatt multi-angle light scattering system determines MW of each protein species autosampler - allows multiple sample setup	Inquire
Differential scanning fluorimetry (DSF) - for 1 sample performed in triplicate	Experiment setup Data collection Data analysis and reporting	Inquire
Analytical ultracentrifugation experiment (per run - up to 7x samples)	Experiment design and setup Instrument usage/maintenance Data analysis and reporting	Inquire
▼ ► Binding studies (5)
Name	Description	Price
Isothermal titration calorimetry (ITC) - per experiment	label free binding studies (no labeling artifacts) determine Kd, stoichiometry, and thermodynamics (enthalpy, entropy and free energy) Experiment Definition: One titration of ligand into protein AND a titration of the ligand into buffer as the heat of dilution control experiment.	Inquire
Microscale Thermophoresis (MST) - experiment design and sample preparation	Experiment planning and design Protein labeling with a fluorophore Purification of labeled protein from free dye Testing capillary compatibility	Inquire
Microscale Thermophoresis (MST) - per experiment	Experiment definition: Record thermophoresis data at 3x MST powers (30%, 60% and 90%) Experiment setup Instrument use and consumables Data fitting and reporting	Inquire
Octet (Biolayer interferometry) - experiment design and sample preparation	Experiment planning and design Protein labeling with a biotin Purification of labeled protein from free biotin	Inquire
Octet (Biolayer interferometry) - per ligand (experiment)	Octet (Biolayer interferometry) - per ligand (experiment)	Inquire
▼ ► Instrument training and daily use (4)
Name	Description	Price
Independent Instrument Use Daily Fee (Biomek FX liquid handler)	Independent Instrument Use Daily Fee Beckman Biomek Fx system for 96 well or 384 well dispensing	Inquire
Independent Instrument Use Daily Fee (ITC, MST, Octet & DSF)	Isothermal titration calorimetry (TA instruments affinity ITC system) Microscale thermophoresis (Nanotemper) Octet/Biolayer interferometry (Pal Forte Bio) Differential scanning fluorimetry	Inquire
Training Fee (ITC, MST, Octet or DSF)		Inquire
Octet biochip (per chip)	Pal ForteBio Octet Biochip	Inquire