Welcome!
Overview of Services
The Department of Pharmacology and Toxicology Core Facilities are multiuser, communal research laboratories that total an approximate 4470 sq. ft. of research space maintained by the department. These facilities are designed to aid and advance the research, in an efficient manner, of individual investigators. The underlying goals of the Core Facilities are to maximize research space, reduce instrumental redundancy, while providing centralized oversight for instrumental maintenance, validity and optimization. The Core Facilities provide these services at cost to the investigator.
Core Equipment is found in one of the designated Core Facility rooms (listed below with PHM/TOX as the desigantion). Core Equipment may be used by departmental and external researchers, once appropriately trained and authorized. The Core Equipment Definition attributes responsibilities on behalf of the Core Facilities to calibrate and maintain the working status of the equipment. All users, after training and being provided training materials, are responsible for their use of the equipment. User error resulting in damage exceeding $500.00 wil be responsible for repairs.
The MSU Assay Development and Drug Repurposing Core (ADDRC) facility located in the Department of Pharmacology and Toxicology was created to assist MSU researchers with bridging the drug discovery gap that often exists between their basic research and preclinical drug development. The primary focus of the ADDRC is to aid investigators in developing HTS-compatible assays, to generate preliminary data for grants and funding opportunities, and to screen biological targets with the goal of providing chemical probes to further interrogate a particular biological process or potential leads for drug development.
The ADDRC will be equipped with compound screening libraries on the order of 35,000 compounds, including about 1000 FDA-approved drugs with the aim of identifying new indications for existing clinical compounds or drug repurposing. The facility possess a variety of 96/384-tip liquid handlers, bulk liquid dispensers, and multi-format plate readers with the capacity to address multiple assay methodologies. Faculty also have access to a microscopy-based high-throughput instrument for automated high content imaging and analysis of fixed or live cells. And most recently, the ADDRC facility has acquired a functional drug screening system (FDSS) , a state-of-the-art imaging plate reader for kinetic cell-based assay development and HTS. While the ADDRC is located in the Life Sciences Building (B411), it is a shared resource established to serve faculty across the MSU campus. In addition, the involvement of undergraduate/graduate students and postdoctoral scientists from investigators’ laboratories, who are interested in learning more about drug discovery and the principles of high-throughput screening, is strongly encouraged.
*The ADDRC facility was established through the generous support of the Vice President for Research and Graduate Studies, the Pharmacology and Toxicology Department, CNS, COM, CHM, CVM, and AgBioResearch.
The mission of the MSU Flow Cytometry Core Facility is to provide investigators with cell sorting services and access to cutting-edge analytical flow cytometry instrumentation, as well as training and experimental consultation. We are graciously supported by the MSU Office of the Senior Vice President for Research & Innovation (VPRI), the College of Human Medicine (CHM), and the College of Osteopathic Medicine (COM).
The MSU South Campus Flow Cytometry Core hub is located on the second floor of the IQ Bioengineering Building (Rm 2521 and 2522). Instrumentation available in IQ include a BD FACSAria IIu cell sorter, a Cytek Aurora spectral cytometer (5 laser), a BD Accuri C6, and a Luminex 200. The MSU North Campus Flow Cytometry Core hub is located on the fifth floor of the Biophysical Science Building (BPS, Rm 5115 and 4120). Instrumentation available in BPS include a BD Influx cell sorter, a ThermoFisher Scientific Attune CytPix, and a BD LSR II benchtop analyzer. Laboratory space for the South Campus hub is approximately 300 sq. ft. and approximately 263 sq. ft. for the North Campus hub. The MSU Flow Cytometry Core is directed by Dr. Matthew Bernard who both provides professional oversight of the Core Facility and assay development expertise, as well as technical support and guidance for general education in flow cytometry principles. The Core is managed by Dr. Daniel Vocelle, who oversees instrument and data analysis training, assay/application development, as well as technical support and guidance for general education in flow cytometry principles.
Anne Dorrance, Ph.D.
Chair & Professor Department of Pharmacology & Toxicology
B440 & B340 Life Science
Email: dorranc3@msu.edu
Phone: 517-432-7403
Fax: 517-353-8915
Erika Lisabeth, Ph.D.
Director, Assay Development and Drug Repurposing Core
B338 Life Science
Email: matheser@msu.edu
Phone: 517-432-4507
Fax: 517-884-4998
Heather de Feijter-Rupp, B.S.
Core Facilities Officer, Comprehensive Research Cores
Department of Pharmacology & Toxicology B142 Life Science
Email: rupph@msu.edu
Phone: 517-884-8023
Matthew Bernard B.S., Ph.D.
Director, MSU Flow Cytometry Core Facility
Email: mbernard@msu.edu
Phone: 517-355-4076
Fax: 517-353-8915
Daniel Vocelle B.S., Ph.D.
Assistant Director, MSU Flow Cytometry Core Facility
Email: vocelled@msu.edu
Phone: 517-355-1536
Fax: 517-353-8915
| Hours | Location |
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Staff Hours: |
1355 Bogue St. Life Science |
| Hours | Location |
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Staff Hours: |
1355 Bogue St. Life Science |
MSU Flow Cytometry Core
| Hours | Location |
|
Staff Hours: |
775 Woodlot Dr, IQ/Bioengineering Facility |
| Hours | Location |
|
Staff Hours: |
567 Wilson Rd, BPS |
| Pricing |
| ► Flow Cytometry Core Facility (1) | |||
| Name | Description | Price | |
|---|---|---|---|
| Flow Cytometry Core Facility External Consultation |
External
$95.00
each
|
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| ► Monthly Equipment Usage (7) | |||
| Name | Description | Price | |
| Autoclave Core (B409) |
This core is currently processing all materials to be sterilized and or autoclaved for disposal. Plese use the form to request service. |
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| Core Cold Rooms (B302, B402, B415) |
Cold rooms provide space for short term storage and gel transfers. Each cold room has a bead bullet homogenizer. |
Internal
$32.51
month
External $40.96 month |
|
| Dark Room Core (B410A) |
For those desiring to process blots with film, we have cassettes, and some film. This system is serviced regularily. |
Internal
$156.12
month
External $196.71 month |
|
| Large Equipment Core (B303) |
This core has space for bacterial work, 2 cryostats and large capacity centrifuges as well as homogenizers. |
Internal
$219.88
each
External $277.04 each |
|
| Microscopy Core (B411) |
Nikon Large Field of View Ti2-E Inverted 5-line (DAPI/GFP/TxRed/Cy5/Cy7) LED, trigger enabled shutter and multiple objetives (2X, 4X, 10X, 20X (DIC) ELWD 40x (DIC) and 60X oil). This core also has an older Nikon and and an Olympus, both with fluorescence capabilities. |
Internal
$334.53
month
External $421.51 month |
|
| Survival Surgery Core (B13) | Inquire | ||
| Tissue Culture Cores (B300, B407) |
Each core has space for storing supplies while working in the cores. There are multiple hoods, sertified yearly, and tested for mycoplasma contamination. The cores have 18ohm water, centrifuge and incubators that are maintained by the sore staff. |
Internal
$278.25
month
External $347.81 month |
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| ► Autoclave Core (2) | ||||||||||||||||||
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| ► MSU FLOW CYTOMETRY CORE (5) | ||||||||||||||||||
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| ► Microscopy Core (1) | ||||||||||||||||||
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| ► Molecular and Biochemistry Core (3) | ||||||||||||||||||
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| ► Tissue Culture Core (1) | ||||||||||||||||||
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