Recombinant Protein Production and Characterization Core

Services

Recombinant Protein Production and Characterization Core offers following major services

1) Expression of recombinant proteins of interest

Expression systems include E. coli, insect cells, mammalian cells (HEK293).

2) Custom protein purification services (IMAC, SEC, IEX, Affinity resins).

Recombinant proteins of interest as well as monoclonal antibodies produced from existing hybridomas

3) Molecular Protein Characterization

Octet, AUC (Analytical Ultracentrifugation), Auto-ITC (Isothermal Titration Calorimetry), DSF (Differential Scanning Fluorimetry)

Core Directors:

Patrick M. Suess, PhD: patrick.suess@bcm.edu

Josephine Ferreon, PhD: josephine.ferreon@bcm.edu

Core Email Alias: atc-recombinant-protein@bcm.edu

Locations, Contacts & Hours

Room R551E & Room N520

1 Baylor Plz, Houston, TX 77030

Mon-Fri 8:30am-4:30pm

____________

Map For Room R551E

Core Website:

Please Visit Our Website for more details!

Contacts

Name	Role	Phone	Email	Location
Josephine Ferreon	Core Director (Protein Characterization)	(713) 798-1756	josephine.ferreon@bcm.edu	Alkek N520.03
Patrick Suess	Core Director	(832) 877-1195	Patrick.Suess@bcm.edu	ABBR R506
Core Email Alias			atc-recombinant-protein@bcm.edu

Service List and Pricing

Name	Description	Price
25ml pilot production		Internal BCM $95.00 each External $152.00 each
2L protein production (without purification)		Internal BCM $134.00 each External $214.00 each
50ml pilot expression		Internal BCM $25.00 each External $40.00 each
Antibody Affinity (Protein A/G) purification (from 2L hybridoma culture)		Internal BCM $1,670.00 each External $2,672.00 each
Desalting		Internal BCM $150.00 each External $240.00 each
Dialysis (per 50ml of sample)		Internal BCM $100.00 each External $160.00 each
Expression and purification from E. coli (per 2L culture)		Internal BCM $847.00 each External $1,335.20 each
Expression Construct Design and Consultation		Inquire
Protein expression 500ml culture		Internal BCM $1,857.00 each External $2,971.00 each
Protein Expression and Purification from E. coli		Internal BCM $847.00 each External $1,355.20 each
Protein Expression from HEK293- 120 ml	Transient transfection in suspension HEK293 culture (120 ml scale)	Internal BCM $453.00 each External $724.80 each
Protein Purification from Insect and Mammalian Cells		Internal BCM $713.90 each External $1,142.24 each
Secondary purification (SEC or IEX, per run)		Internal BCM $396.00 each External $633.00 each
Spinner Vessel Protein Production 500ml		Internal BCM $350.00 each External $560.00 each
Western on pilot production against affinity tag		Internal BCM $90.00 each External $144.00 each
Western on pilot samples against affinity tag		Internal BCM $90.00 each External $144.00 each
Western on pilot samples against affinity tag		Internal BCM $90.00 each External $144.00 each

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Baculovirus (2)
Western on pilot production against affinity tag		Internal BCM $90.00 each External $144.00 each
Spinner Vessel Protein Production 500ml		Internal BCM $350.00 each External $560.00 each
▼ ► Monoclonal Antibody Production (1)
Name	Description	Price
Antibody Affinity (Protein A/G) purification (from 2L hybridoma culture)		Internal BCM $1,670.00 each External $2,672.00 each
▼ ► Protein Purification (5)
Name	Description	Price
Desalting		Internal BCM $150.00 each External $240.00 each
Dialysis (per 50ml of sample)		Internal BCM $100.00 each External $160.00 each
Secondary purification (SEC or IEX, per run)		Internal BCM $396.00 each External $633.00 each
Protein Expression and Purification from E. coli		Internal BCM $847.00 each External $1,355.20 each
Protein Purification from Insect and Mammalian Cells		Internal BCM $713.90 each External $1,142.24 each
▼ ► Experimental Design and Consultation (1)
Name	Description	Price
Expression Construct Design and Consultation		Inquire
▼ ► HEK293 (4)
Name	Description	Price
25ml pilot production		Internal BCM $95.00 each External $152.00 each
Protein expression 500ml culture		Internal BCM $1,857.00 each External $2,971.00 each
Western on pilot samples against affinity tag		Internal BCM $90.00 each External $144.00 each
Protein Expression from HEK293- 120 ml	Transient transfection in suspension HEK293 culture (120 ml scale)	Internal BCM $453.00 each External $724.80 each
▼ ► Protein expression in bacteria (E. coli) (4)
Name	Description	Price
2L protein production (without purification)		Internal BCM $134.00 each External $214.00 each
50ml pilot expression		Internal BCM $25.00 each External $40.00 each
Expression and purification from E. coli (per 2L culture)		Internal BCM $847.00 each External $1,335.20 each
Western on pilot samples against affinity tag		Internal BCM $90.00 each External $144.00 each