Welcome!
Recombinant Protein Production and Characterization Core offers following major services
1) Expression of recombinant proteins of interest
Expression systems include E. coli, insect cells, mammalian cells (HEK293).
2) Custom protein purification services (IMAC, SEC, IEX, Affinity resins).
Recombinant proteins of interest as well as monoclonal antibodies produced from existing hybridomas
3) Molecular Protein Characterization
Octet, AUC (Analytical Ultracentrifugation), Auto-ITC (Isothermal Titration Calorimetry), DSF (Differential Scanning Fluorimetry)
Patrick M. Suess, PhD: patrick.suess@bcm.edu
Josephine Ferreon, PhD: josephine.ferreon@bcm.edu
Core Email Alias: atc-recombinant-protein@bcm.edu
Room R551E & Room N520
1 Baylor Plz, Houston, TX 77030
Mon-Fri 8:30am-4:30pm
____________
Map For Room R551E
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Josephine Ferreon |
Core Director (Protein Characterization)
|
(713) 798-1756
|
josephine.ferreon@bcm.edu
|
Alkek N520.03
|
| Patrick Suess |
Core Director
|
(832) 877-1195
|
Patrick.Suess@bcm.edu
|
ABBR R506
|
| Core Email Alias |
atc-recombinant-protein@bcm.edu
|
| Service List and Pricing |
| ► Baculovirus (1) | |||
| Name | Description | Price | |
|---|---|---|---|
| Western on pilot production against affinity tag |
External
$144.00
each
|
||
| ► Monoclonal Antibody Production (1) | |||
| Name | Description | Price | |
| Antibody Affinity (Protein A/G) purification (from 2L hybridoma culture) |
External
$2,672.00
each
|
||
| ► Protein Purification (3) | |||
| Name | Description | Price | |
| Desalting |
External
$240.00
each
|
||
| Dialysis (per 50ml of sample) |
External
$160.00
each
|
||
| Secondary purification (SEC or IEX, per run) |
External
$633.00
each
|
||
| ► HEK293 (3) | |||
| Name | Description | Price | |
| 25ml pilot production |
External
$152.00
each
|
||
| Protein expression 500ml culture |
External
$2,971.00
each
|
||
| Western on pilot samples against affinity tag |
External
$144.00
each
|
||
| ► Protein expression in bacteria (E. coli) (4) | |||
| Name | Description | Price | |
| 2L protein production (without purification) |
External
$214.00
each
|
||
| 50ml pilot expression |
External
$40.00
each
|
||
| Expression and purification from E. coli (per 2L culture) |
External
$1,335.20
each
|
||
| Western on pilot samples against affinity tag |
External
$144.00
each
|
||
