X-Ray Crystallography and Macromolecular Characterization Core

Overview of Services

1. Provide homogeneous and well characterized protein for biochemical researches
2. Provide structural information to understand mechanisms of biological systems at the atomic level
3. Validate binding sites of lead compounds of therapeutic interest
4. Facilitate lead discovery through co-crystallization of therapeutic targets and small molecule libraries
    

• Protein expression in E. coli, insect cells (baculovirus-mediated expression Hi5 cells), and mammalian cells
• Protein purification using a large variety of chromatographic techniques 
• Protein charaterization using biophysical approaches
• Protein crystallization
• Diffraction quality
• Data collections
• Structure determination

Research reported in this publication included work performed in the X-ray Crystallography Core supported by the National Cancer Institute of the National Institutes of Health under award number P30CA33572. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Structure Gallery

Leadership

Location and hours of operation

Links and Resources

COH Website:

http://www.cityofhope.org/x-ray-crystallography-core-facility

SPR links:

SPR Basics 

https://www.biacore.com/lifesciences/index.html

AUC links:

AUC 101  (video)

https://sedfitsedphat.nibib.nih.gov/software/default.aspx

ITC links:

http://www.currentprotocols.com/WileyCDA/CPUnit/refId-cb1708.html

http://www.enzim.hu/febs2009/pdfs/Lewis_ITC.pdf

http://biophysics.swmed.edu/MBR/software.html

Protein Crystallography links:

https://hamptonresearch.com/Default.aspx

CD Spectroscopy links:

http://www.photophysics.com/tutorials/circular-dichroism-cd-spectroscopy/1-understanding-circular-dichroism

www.jasco.com

Kinetic Exclusion Assay (KinExA) links:

https://www.sapidyne.com

Contacts

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