Flow Cytometry Facility

Overview of Services

 

The CRL Flow Cytometry Facility operates two cores. in the Life Sciences Addition (LSA) room 491 and in the Li Ka Shing (LKS) room 461.

The LSA 491 core houses two cell sorters and four analyzers.

The LKS 461 core houses one cell sorter and one analyzer. This is a CIRM shared flow core.

The CRL Flow Cytometry Facility instrumentation provides light scattering and fluorescent based measurements of single cells by laser excitation. Applications of this technology include identification of sub-populations of cells by means of immunofluorescence, gene reporting using GFP, YFP, RFP CFP and M-Cherry, DNA content, as well as functional Ca++ Flux, and cell viabilities.

Populations identified by the applications mentioned above can be separated and purified by cell sorting. Cells can be sorted in bulk (up to 6 way sorting) or by single cell deposition into 96 well plates (other plate templates like 6, 24, and 48 well plates are also supported).

 

Use of the flow cytometry facility instrumentation is by appointment only.

Leadership

 

Hector Nolla, Core Manager

hectorno@berkeley.edu

 

Kartoosh Heydari, MD. Core Manager

kartooshheydari@berkeley.edu

 

Alma Valeros, Sort Operator

avaleros@berkeley.edu

 

Location and hours of operation

 

Hours Location

9:00 am to 9:00 pm (LSA)     

9:00 am- 5:00 pm (LKS)

(Analyzers are available 24/7 to trained users)      

LSA 491

LKS 461 

Links and Resources

 

crl.berkeley.edu

 

Contacts

Name Role Phone Email Location
Hector Nolla
Core Manager and Sr. Operator, LSA
 
(510) 642-2843
 
hectorno@berkeley.edu
 
Flow Facility (LSA)
 
Alma Valeros
Staff Res. Assoc
 
(510) 642-2843
 
avaleros@berkeley.edu
 
Flow Facility (LSA)
 
Kartoosh Heydari, MD
Core Director/Manager LKS
 
(510) 664-4308
 
kartooshheydari@berkeley.edu
 
461 Li Ka Shing Center