Next-generation Sequencing
BeadChip Arrays
Droplet Digital PCR (ddPCR)
Single Cell and Spatial services
Single Cell Sequencing
Spatial Transcriptomics
Marie Adams, M.S., MB(ASCP)CM - Director
Marc Wegener, B.S.
Katelyn Becker, M.S.
Tracey Avequin, B.S.
Drew Hankiewicz, B.S.
Brandon Compton
Brennan Grimes, B.S.
Location and Hours of Operation
| Hours | Location |
|
8AM - 5PM |
Van Andel Research Institute 333 Bostwick Avenue, NE Phase II, Level 1 Grand Rapids, MI 49503 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Marie Adams, M.S. |
Core Director
|
616-234-5535
|
marie.adams@vai.org
|
1217W
|
| Marc Wegener |
Senior Core Associate
|
616-234-5826
|
marc.wegener@vai.org
|
1213W
|
| Katelyn Becker |
Core Laboratory Manager
|
616-234-5726
|
katelyn.becker@vai.org
|
1213W
|
| Tracey Avequin |
Core Associate
|
616-234-5740
|
tracy.avequin@vai.org
|
1213W
|
| Drew Hankiewicz |
Core Associate
|
616-234-5585
|
andrew.hankiewicz@vai.org
|
1213W
|
| Brandon Compton |
Core Associate
|
brandon.compton@vai.org
|
1213W
|
|
| Brennan Grimes |
Core Associate
|
brennan.grimes@vai.org
|
1213W
|
| Service Cost |
| ► Genomics (5) | |||
| Name | Description | Price | |
|---|---|---|---|
| iDOT Plate Purchase |
Pass through cost per plate. |
Inquire | |
| Mosquito Tips |
Per tip cost for 4.5mm and 9mm tips |
Inquire | |
| Nanopore Minion 9.4.1 Flowcell |
Pass-through purchase of Nanopore Minion flowcell for lab use |
Inquire | |
| Re-Demultiplexing Charge |
For projects where the wrong indexes were provided. This charge is designed to account for additional analysis time by the Core. |
Inquire | |
| UDI Adapters (per adapter) |
5uL of 3 uL unique dual indexed adapter (96 available combinations) for adapter-ligation based NGS libraries. |
Inquire | |
| ► Sequencing Library Preparation (14) | |||
| Name | Description | Price | |
| 10X Genomics Flex Sample QC (per Sample) |
QC of fixed cells or nuclei pre and post hybridization |
Inquire | |
| 10X Genomics FLEX scRNA library prep 1x4 (per sample) | Inquire | ||
| 10X Genomics FLEX scRNA library prep 4x4 (per 2-4 sample pool) | Inquire | ||
| 10X Genomics FLEX scRNA library prep 16x16 (per 5-16 sample pool) | Inquire | ||
| 10X Genomics FLEX scRNA library prep 16x4 (per 5-16 sample pool) | Inquire | ||
| 10X Genomics single cell Project Setup (Live Cell, 3' , 5' and scATAC only) |
Setup cost for 1-8 samples. Setup cost repeats every 8 samples |
Inquire | |
| 10x scRNA Library Prep (per sample) |
to be used alongside Project setup fee. |
Inquire | |
| 10X ADT/HTO/CITE processing | Inquire | ||
| smallRNA/miRNAseq Library Prep |
per sample charge |
Inquire | |
| Genomic DNA (WGS) Library Preparation | Inquire | ||
| Agilent v6 Exome Capture (16-32 samples) | Inquire | ||
| Agilent v6 Exome Capture (33-64 samples) | Inquire | ||
| Agilent v6 Exome Capture (65-96 samples) | Inquire | ||
| Custom Targeted Panel Labor/Consumable Costs |
Labor and consumables for varied DNA-based capture projects. Kit costs will be charged separately as a pass through item. |
Inquire | |
| ► Sequencing: Illumina Runs (12) | |||
| Name | Description | Price | |
| NovaSeq SP 200 cycle |
Used for 2x100 bp sequencing runs; produces 0.8 B seq fragments, and ~150 Gbases per flowcell. |
Inquire | |
| NovaSeq S1 100 cycle |
Used for either 1x100 or 2x50 bp sequencing runs; produces 1.5B seq fragments, and ~150 Gbases per flowcell. |
Inquire | |
| NovaSeq S1 200 cycle |
Used for 2x100 bp sequencing runs; produces 1.5B seq fragments, and ~300 Gbases per flowcell. |
Inquire | |
| Novaseq S1 300 cycle |
Used for 2x150 bp sequencing runs; produces 1.6 B seq fragments, and ~450 Gbases per flowcell. |
Inquire | |
| NovaSeq S2 100 cycle |
Used for either 1x100 or 2x50 bp sequencing runs; produces 3B seq fragments, and ~300 Gbases per flowcell. |
Inquire | |
| NovaSeq S2 200 cycle |
Used for 2x100 bp sequencing runs; produces 3B seq fragments, and ~600 Gbases per flowcell. |
Inquire | |
| NovaSeq S2 300 cycle |
Used for 2x150 bp sequencing runs; produces 3B seq fragments, and ~900 Gbases per flowcell. |
Inquire | |
| NovaSeq S4 300 cycle |
Used for either 2x150 bp sequencing runs; produces 10B seq fragments, and ~3 Tbases per flowcell. Spedifically designed for Whole Genome and Whole Exome Sequencing. |
Inquire | |
| NovaSeq6000 2x50 split lane, 50-400M reads (per 50M reads) |
Charged in increments of 50M reads, to a maximum of 400M reads |
Inquire | |
| NovaSeq6000 2x50 split lane, 400-1600M reads (per 50M reads) |
Charged in increments of 50M reads, 400-1600M reads |
Inquire | |
| NovaSeq6000 2x100 split lane. 50-400M reads (per 50M reads) |
Charged in increments of 50M reads, to a maximum of 400M reads |
Inquire | |
| NovaSeq6000 2x100 split lane, 400-1600M reads (per 50M reads) |
Charged in increments of 50M reads, 400M-1600M reads |
Inquire | |
| ► iScan Arrays (6) | |||
| Name | Description | Price | |
| Infinium Global Diversity Array (GDA), 96+ samples |
Replaces discontinued Infinium MEGA array |
Inquire | |
| Mouse Methyl Array (24 or fewer) |
Samples must be submitted in multiples of 12 to receive this rate |
Inquire | |
| Mouse Methyl Array (36-84 samples) |
Samples must be submitted in multiples of 12 to receive this rate |
Inquire | |
| Mouse Methyl Array (96+) |
Samples must be submitted in multiples of 12 to receive this rate |
Inquire | |
| iScan Infinium HD EPIC Array oxBS/BS Add-on (8 samples, 16 reactions) | Inquire | ||
| Custom iSelect Array (48) – Labor and Consumables |
Kits purchased directly by lab for custom orders and shipped to VAI core facility |
Inquire | |
| ► Spatial Transcriptomics (14) | |||
| Name | Description | Price | |
| 10X Xenium Processing (per slide) |
Note: two slides must be run in tandem to receive this pricing. Enquire about single slide pricing if necessary. |
Inquire | |
| GeoMx DSP Setup Labor (1-4 slides) |
Applicable for morphology check or ROI collection. |
Inquire | |
| Morphology Check Consumables (per slide) | Inquire | ||
| ROI selection: Initial 24 ROIs (per plate) | Inquire | ||
| ROI selection: + 24 ROIs |
Additional 24 ROIs above base 24 |
Inquire | |
| ROI Selection: + 48 ROIs |
Additional 24 ROIs above base 24 |
Inquire | |
| ROI selection: +72 ROIs |
Additional 72 ROIs above base 24 |
Inquire | |
| ROI selection: Rare Cell Profiling |
Additional charge for time needed to complete complex ROI selection motifs |
Inquire | |
| GeoMx RNA NGS Library preparation (per 96 well plate) |
Library preparation, QC, pooling and normalization of project AOIs |
Inquire | |
| NGS per AOI charge |
Cost per individual AOI barcode (direct passthrough) |
Inquire | |
| GeoMx Whole Transcriptome Atlas (Human or Mouse) |
Pass through charge for baits only |
Inquire | |
| GeoMx Cancer Transcriptome Atlas (Human or Mouse) |
Pass through charge for baits |
Inquire | |
| Protein Assay |
Pass through charge for core baits only. Additional project specific antibody baits purchased by lab. |
Inquire | |
| Spatial Transcriptomics Sequencing (per 50M reads) | Inquire | ||
| ► Z Inactive (5) | |||
| Name | Description | Price | |
| iScan OmniExpressExome (16 samples) | Inquire | ||
| Infinium MEGA Array (16) | Inquire | ||
| iScan OmniExpressExome (48 samples) | Inquire | ||
| Infinium MEGA Array (32) | Inquire | ||
| Infinium MEGA Array (96) | Inquire | ||
| Name | Price | |
|---|---|---|
| iDot Liquid Handler | View calendar | |
| Mosquito HTS Liquid Handler | View calendar | |
| QX200 Automated Droplet Generator (Auto DG)/C1000 Touch Thermal Cycler | View calendar | |
| ZZ Archived Equipment: 10X Chromium Controller | View calendar | |
| ZZ Archived Equipment: Nanostring GeoMx (ROI selection and analysis) | View calendar | |