Welcome!
ALERT ATTENTION ⚠️
In Observance of the holidays, Agilent CrossLab/ iLab Operations Software Support Help Desk will be closed during U.S. hours on Wednesday January 1st, 2025. We will resume regular U.S. support hours on Thursday January 2nd, 2025. For urgent matters, please add "Urgent" to the ticket/ email subject or press "1", when prompted, to escalate a call on the iLab Support phone and we will prioritize those requests first.
The IIDS Genomics and Bioinformatics Resources Core provides researchers from the University of Idaho access to technology, experience, and expertise in molecular biology methods. The core utilizes advanced technologies from companies such as Illumina, PacBio, Oxford Nanopore, Fluidigm, Sage Biosciences, and Advanced Analytical. Applications of these technologies are commonly used in biomedical research, but the interdisciplinary focus of the University of Idaho results in a wide range of fields that can benefit from the core, including agricultural studies, forest management, and animal science. The Genomics Resources core is also available to researchers that are external to the University of Idaho, which expands our collaborative base.
The core offers expertise in the bioinformatics needed to acquire, analyze, and visualize data generated from high throughput technologies used in genomics research. With approximately $1.8 million in specialized genomic equipment, we offer a wide variety of services and capabilities including next-generation sequencing with Illumina technologies, targeted re-sequencing, and high-throughput sample preparation of both DNA and RNA.
The Genomics and Bioinformatics Resources Core implements an innovative approach to Core Facility management: the Interdisciplinary Triangle of Collaboration. The key to success of the Interdisciplinary Triangle of Collaboration is its holistic approach. Communication between all key personnel (Principal Investigator, Bioinformatician, and Molecular Scientist) occurs from at all stages of the experimental process, starting with study planning, design, and data generation through data interpretation, visualization, and manuscript preparation. The Core provides the specialized technical expertise, extensive experience, and equipment as a part of a productive relationship with the researchers. This uncommon and innovative approach to core facility management allows the IIDS Genomics and Bioinformatics Resources Core to provide customized technical expertise that most single investigators would not have access to.
The IIDS Genomics and Bioinformatics Resources Core specializes in:
The GBRC is funded in part by an NIH COBRE grant (P30GM103324) so all papers, publications and press releases resulting from the use of the Genomics and Bioinformatics Resources Core facility should abide by the Fair Access to Science and Technology and NIH Open Access policies. They should also include the following acknowledgement:
“Data collection and analyses performed by the IIDS Genomics and Bioinformatics Resources Core at the University of Idaho.”
Additionally, if services you received from the GBRC included significant intellectual contributions (including consulting, experimental design, or troubleshooting/developing laboratory or bioinformatic methods), authorship or acknowledgement for the appropriate staff member(s) is required in associated publications. For further clarification of authorship questions, please see the ABRF Guidelines.
Sarah Hendricks, Bioinformatic Data Scientiest
shendricks@uidaho.edu
208-885-6107
Dan New, Lab Manager
dnew@uidaho.edu
208-885-7023
Maria Schneider, Lab Coordinator
gmarias@uidaho.edu
208-885-7023
|
Contact the Genomics and Bioinformatics Resources Core before shipping: 208-885-7023 |
Click here for full size image
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Dan New |
Lab Manager
|
208-885-7023
|
dnew@uidaho.edu
|
IRIC 210
|
| Maria Schneider |
Lab Coordinator
|
208-885-7023
|
gmarias@uidaho.edu
|
IRIC 210
|
| Sarah Hendricks |
Bioinformatic Data Scientist
|
208-885-6107
|
shendricks@uidaho.edu
|
IRIC 224
|
| Projects, Services, and Quote Requests |
| ► FA, qualification and quantification, Bioanalyzer equivalent (but better) (6) | |||
| Name | Description | Price | |
|---|---|---|---|
| Fragment Analyzer (AATI), NGS library qualification |
|
Internal
$36.00
each
External $44.00 each Corporate $60.00 each |
|
| Fragment Analyzer (AATI), NGS library qualification (ENTIRE ROW, 11samples) |
|
Internal
$118.00
each
External $142.00 each Corporate $197.00 each |
|
| Fragment Analyzer (AATI) High Sensitivity gDNA, 50k |
|
Internal
$36.00
each
External $44.00 each Corporate $60.00 each |
|
| Fragment Analyzer (AATI) High Sensitivity gDNA, 50k (ENTIRE ROW, 11 samples) |
|
Internal
$118.00
each
External $142.00 each Corporate $197.00 each |
|
| Fragment Analyzer (AATI) Total RNA |
|
Internal
$36.00
each
External $44.00 each Corporate $60.00 each |
|
| Fragment Analyzer (AATI) Total RNA (ENTIRE ROW, 11samples) |
|
Internal
$118.00
each
External $142.00 each Corporate $197.00 each |
|
| ► Sample Quantification and Qualification (10) | |||
| Name | Description | Price | |
| Library Preparation and Quantification (qPCR) |
|
Internal
$39.00
each
External $47.00 each Corporate $65.00 each |
|
| Qubit High Sensitivity DNA Quantification |
Quantified double-stranded DNA, 10pg/uL to 100ng/uL
Prices are per sample |
Internal
$3.00
each
External $4.00 each Corporate $5.00 each |
|
| Qubit Broad Range RNA Quantification |
quantified RNA from 100 pg⁄µl to 1000 ng⁄µl, destructive testing Prices are per sample |
Internal
$3.00
each
External $4.00 each Corporate $5.00 each |
|
| Qubit Broad Range DNA Quantification |
quantified double-stranded nucleic acid 100 pg/µL to 1000 ng/µL, destructive testing
Prices are per sample |
Internal
$3.00
each
External $4.00 each Corporate $5.00 each |
|
| NanoDrop |
Please let us know which buffer you used to elute your sample. |
Internal
$4.00
each
External $4.80 each Corporate $6.65 each |
|
| Pooling Charge |
A one-time charge for technician time involved in pooling amplicons. |
Internal
$21.00
each
External $26.00 each Corporate $35.00 each |
|
| Plate - DNA Fluorometry |
91 samples per plate - leave wells H8-H12 empty. |
Internal
$80.00
each
External $96.00 each Corporate $134.00 each |
|
| Plate - RNA Fluorometry |
91 samples per plate - leave wells H8-H12 empty. |
Internal
$80.00
each
External $96.00 each Corporate $134.00 each |
|
| Turner DNA Fluorometry |
Prices are per sample |
Internal
$7.00
Sample
External $9.00 Sample Corporate $12.00 Sample |
|
| Turner RNA Fluorometry |
Prices are per sample |
Internal
$7.00
each
External $9.00 each Corporate $12.00 each |
|
| ► DNA Library (20) | |||
| Name | Description | Price | |
| Affordable Illumina DNA Shotgun-Libraries, 1 or more |
Manual production of a minimum of 8 shotgun-libraries including everything but sequencing @ UI. |
Internal
$363.00
each
External $436.00 each Corporate $604.00 each |
|
| Affordable Illumina DNA Shotgun-Libraries, 8 or more |
Manual production of a minimum of 8 shotgun-libraries including everything but sequencing @ UI. |
Internal
$96.25
each
External $115.50 each Corporate $160.13 each |
|
| Affordable Illumina DNA Shotgun-Libraries, 16 or more libraries |
Manual production of a minimum of 16 shotgun-libraries including everything but sequencing @ UI.
|
Internal
$74.06
each
External $88.88 each Corporate $123.19 each |
|
| Affordable Illumina DNA Shotgun-Libraries, 48 or more libraries |
Manual production of a minimum of 48 shotgun-libraries including everything but sequencing @ UI. |
Internal
$54.94
each
External $65.94 each Corporate $91.38 each |
|
| Illumina Shotgun Library, Covaris and Ligate, 1 library |
Input: non QC'ed DNA that is ready for QC <= 1,000ng to ligation Output: Pooled, qubited, FA'ed, and test-PCR'ed library ready to ship |
Internal
$682.82
each
External $820.00 each Corporate $1,136.00 each |
|
| Illumina Shotgun Library, Covaris and Ligate, 8 libraries |
Input: non QC'ed DNA that is ready for QC <= 1,000ng to ligation Output: Pooled, qubited, FA'ed, and test-PCR'ed library ready to ship |
Internal
$1,150.56
each
External $1,381.00 each Corporate $1,914.00 each |
|
| Illumina Shotgun Library, Covaris and Ligate, 16 libraries |
Input: non QC'ed DNA that is ready for QC <= 1,000ng to ligation Output: Pooled, qubited, FA'ed, and test-PCR'ed library ready to ship |
Internal
$1,779.13
each
External $2,135.00 each Corporate $2,959.00 each |
|
| Illumina Shotgun Library, Covaris and Ligate, 48 libraries |
Input: non QC'ed DNA that is ready for QC <= 1,000ng to ligation Output: Pooled, qubited, FA'ed, and test-PCR'ed library ready to ship |
Internal
$3,974.54
each
External $4,770.00 each Corporate $6,610.00 each |
|
| Illumina Shotgun DNA Library, PCR-based |
Automated Apollo Illumina DNA library preparation |
Internal
$211.00
each
External $257.00 each Corporate $356.00 each |
|
| Illumina Shotgun DNA Library, PCR-Free |
Manual production of PCR-free shotgun-libraries for Illumina including everything but sequencing @ UI. |
Internal
$203.00
each
External $244.00 each Corporate $338.00 each |
|
| Illumina CHiP-seq Library peparation |
Automated Apollo CHiP-seq library prep, custom insert size |
Internal
$237.00
each
External $285.00 each Corporate $395.00 each |
|
| NREL/Chou PCR-free libraries (DAP-Seq), 1library |
Input: non QC’ed DNA that is ready for QC Output: pooled, qubited, FA’ed, and test-PCRed library ready to ship |
Internal
$856.00
each
External $1,028.00 each Corporate $1,424.00 each |
|
| NREL/Chou PCR-free libraries (DAP-Seq), 2 libraries |
Input: non QC’ed DNA that is ready for QC Output: pooled, qubited, FA’ed, and test-PCRed library ready to ship |
Internal
$954.00
each
External $1,145.00 each Corporate $1,587.00 each |
|
| NREL/Chou PCR-free libraries (DAP-Seq), 3 libraries |
Input: non QC’ed DNA that is ready for QC Output: pooled, qubited, FA’ed, and test-PCRed library ready to ship |
Internal
$1,053.00
each
External $1,264.00 each Corporate $1,752.00 each |
|
| NREL/Chou PCR-free libraries (DAP-Seq), 4 libraries |
Input: non QC’ed DNA that is ready for QC Output: pooled, qubited, FA’ed, and test-PCRed library ready to ship |
Internal
$1,151.00
each
External $1,382.00 each Corporate $1,915.00 each |
|
| NREL/Chou PCR-free libraries (DAP-Seq), 5 libraries |
Input: non QC’ed DNA that is ready for QC Output: pooled, qubited, FA’ed, and test-PCRed library ready to ship |
Internal
$1,249.00
each
External $1,499.00 each Corporate $2,078.00 each |
|
| NREL/Chou PCR-free libraries (DAP-Seq), 6 libraries |
Input: non QC’ed DNA that is ready for QC Output: pooled, qubited, FA’ed, and test-PCRed library ready to ship |
Internal
$1,347.00
each
External $1,617.00 each Corporate $2,241.00 each |
|
| Pooling and QC of 4 or more qPCRed libraries |
A one-time charge for normalzing pPCRed libraries, then FA, qPCR, and normalizing the pool.
|
Internal
$174.00
each
External $209.00 each Corporate $290.00 each |
|
| Library Amplification (KAPA) |
Amplified library using KAPA kits; user must specify number of cycles |
Internal
$22.00
each
External $27.00 each Corporate $37.00 each |
|
| Illumina Mate-Pair Library Prep - Nextera |
Nextera's mate-pair library prep, requires 1ug of unfragmented high-quality genomic DNA. Output is a single mate-pair library ready for Illumina sequencing. Price includes QC of sample before and after library generation. |
Internal
$491.00
each
External $590.00 each Corporate $817.00 each |
|
| ► Fluidigm (6) | |||
| Name | Description | Price | |
| Fluidigm 192.24 (includes post-chip prep) |
192 samples |
Internal
$3,144.00
each
External $4,479.00 each Corporate $6,207.00 each |
|
| Fluidigm 192.24 |
192 samples |
Internal
$2,607.00
each
External $3,129.00 each Corporate $4,336.00 each |
|
| Fluidigm 48x48 well Singleplex (includes post-chip prep) |
48 samples |
Internal
$1,443.00
each
External $2,028.00 each Corporate $2,811.00 each |
|
| Fluidigm 48x48 well Singleplex |
48 samples, singleplex (48 primer pairs) |
Internal
$1,122.00
each
External $1,347.00 each Corporate $1,866.00 each |
|
| Fluidigm 48x48 well Multiplex |
48 samples, more than 48 primer pairs |
Internal
$1,336.00
each
External $1,604.00 each Corporate $2,222.00 each |
|
| Fluidigm sample pooling |
Required if doing fluidigm |
Internal
$350.00
each
External $420.00 each Corporate $583.00 each |
|
| ► Genotyping (library-prep for Illumina Sequencing) (4) | |||
| Name | Description | Price | |
| 24x high density mouse genotyping panel (~1700 SNPs) |
Input: quantifed and qualified DNA |
Internal
$1,005.00
each
External $1,206.00 each Corporate $1,672.00 each |
|
| 48x high density mouse genotyping panel (~1700 SNPs) |
Input: quantified and qualified DNA |
Internal
$1,449.00
each
External $1,739.00 each Corporate $2,410.00 each |
|
| 72x high density mouse genotyping panel (~1700 SNPs) |
Input: quantified and qualified DNA |
Internal
$1,914.00
each
External $2,297.00 each Corporate $3,183.00 each |
|
| 96x high density mouse genotyping panel (~1700 SNPs) |
Input: quantified and qualified DNA |
Internal
$2,380.00
each
External $2,856.00 each Corporate $3,958.00 each |
|
| ► Illumina (9) | |||
| Name | Description | Price | |
| MiSeq Sequencing v3 (600 cycles) |
MiSeq Sequencing Full Run (600 cycles ~20 Million Reads) |
Internal
$3,282.00
each
External $3,939.00 each Corporate $6,440.00 each |
|
| MiSeq Sequencing v3 (150 cycles) |
MiSeq Sequencing Full Run (150 cycles ~20 Million Reads) |
Internal
$2,192.00
each
External $2,631.00 each Corporate $4,301.00 each |
|
| MiSeq Sequencing v2 (500 cycles) |
|
Internal
$2,662.00
each
External $3,195.00 each Corporate $5,223.00 each |
|
| MiSeq Sequencing v2 (300 cycles) |
|
Internal
$2,435.00
each
External $2,922.00 each Corporate $4,778.00 each |
|
| MiSeq Sequencing v2 (50 cycles) |
|
Internal
$2,034.00
each
External $2,441.00 each Corporate $3,991.00 each |
|
| MiSeq Sequencing v2 (Micro 300) |
|
Internal
$1,375.00
each
External $1,650.00 each Corporate $2,698.00 each |
|
| MiSeq Sequencing v2 (Nano 500 cycles) |
|
Internal
$1,218.00
each
External $1,462.00 each Corporate $2,390.00 each |
|
| MiSeq Sequencing v2 (Nano 300 cycles) |
|
Internal
$1,116.00
each
External $1,340.00 each Corporate $2,190.00 each |
|
| Protocol Development (Troubleshooting and/or Sequencing) |
This project template is for researchers who are developing protocols and would like to test them. Troubleshooting protocols can be expensive, so the GRC is offering a customized development template that will take advantage of the staff's extensive experience to provide guidance for the researcher.
The project template includes experimental design consultation, QA/QC for submitted samples, sequencing, and bioinformatics analysis to determine the success of the project. |
Internal
$734.00
each
External $880.00 each Corporate $1,056.00 each |
|
| ► Illumina, RNA Library (20) | |||
| Name | Description | Price | |
| 3' RNA Library, Lexogen, 1library (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$815.00
each
External $978.00 each Corporate $1,356.00 each |
|
| 3' RNA Library, Lexogen, 2libraries (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$436.00
each
External $523.50 each Corporate $725.40 each |
|
| 3' RNA Library, Lexogen, 3libraries (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$310.00
each
External $372.00 each Corporate $515.67 each |
|
| 3' RNA Library, Lexogen, 4libraries (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$247.00
each
External $296.50 each Corporate $411.00 each |
|
| 3' RNA Library, Lexogen, 5libraries (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$209.20
each
External $251.20 each Corporate $348.00 each |
|
| 3' RNA Library, Lexogen, 6libraries (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$183.83
each
External $220.67 each Corporate $305.83 each |
|
| 3' RNA Library, Lexogen, 7libraries (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$165.86
each
External $199.14 each Corporate $275.86 each |
|
| 3' RNA Library, Lexogen, 8libraries (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$152.38
each
External $182.88 each Corporate $253.50 each |
|
| 3' RNA library, Lexogen, 9-17 libraries (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$146.33
each
External $175.67 each Corporate $243.44 each |
|
| 3' RNA library, Lexogen, 18-35 libraries (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$104.22
each
External $125.11 each Corporate $173.33 each |
|
| 3' RNA library, Lexogen, 36-47 libraries (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$82.11
each
External $98.56 each Corporate $136.56 each |
|
| 3' RNA library, Lexogen, 48+ libraries (EACH) |
Stranded RNA library made from polyA RNA only |
Internal
$77.67
each
External $93.20 each Corporate $129.17 each |
|
| Illumina stranded RNA library preparation |
Automated Apollo Illumina stranded RNA library preparation, 1 insert size |
Internal
$322.00
each
External $387.00 each Corporate $536.00 each |
|
| Ribo-Zero ribosomal RNA depletion |
Results in ribo-depleted RNA for stranded workflow or ds-cDNA |
Internal
$157.00
each
External $189.00 each Corporate $262.00 each |
|
| Ribominus ribosomal RNA depletion |
Results in ribo-depleted RNA for stranded workflow or ds-cDNA |
Internal
$139.00
each
External $167.00 each Corporate $232.00 each |
|
| Stranded Illumina RNA library |
Stranded RNA library made by hand from RNA of interest. |
Internal
$236.00
each
External $284.00 each Corporate $393.00 each |
|
| Apollo poly-A mRNA selection |
Automated selection of mRNA using poly-A |
Internal
$63.00
each
External $75.60 each Corporate $104.77 each |
|
| Illumina smRNA library prep |
Automated Apollo small RNA library preparation |
Internal
$342.00
each
External $411.00 each Corporate $569.00 each |
|
| DNAse treatment of RNA |
removes DNA from RNA samples |
Internal
$22.00
each
External $27.00 each Corporate $37.00 each |
|
| Manual ds-cDNA production |
ds-cDNA ready for Illumina DNA library workflow |
Internal
$146.00
each
External $176.00 each Corporate $243.00 each |
|
| ► Oxford NanoPore Technologies (ONT) (2) | |||
| Name | Description | Price | |
| ONT Ligation Barcoded Library (1-6 samples) - CUSTOM ESTIMATE ON REQUEST |
Input: 1000ng HMW DNA for 1 - 6 samples |
||
| ONT Rapid Barcoded Library (1-6 samples) - CUSTOM ESTIMATE ON REQUEST |
Input: 400ng HMW DNA for 1 - 6 samples |
||
| ► Size Selection (5) | |||
| Name | Description | Price | |
| Bead-Based Size Selection |
Size selection using beads |
Internal
$17.00
each
External $21.00 each Corporate $29.00 each |
|
| Gel-cut Size selection |
Internal
$153.00
each
External $184.00 each Corporate $255.00 each |
||
| Blue Pippin size selection 250bp - 1.5kb |
Size select samples using the Blue Pippin 1.5% agarose cassette, between 250 bp and 1.5 kb. Smaller size ranges will have lower yields.
Cost is per sample. A normal cassette cartridge contains 5 sample lanes with internal standards. |
Internal
$122.00
each
External $147.00 each Corporate $203.00 each |
|
| Blue Pippin size selection 10kb - 18kb |
Size select samples using the Blue Pippin 0.75% agarose cassette, between 10 kb and 18 kb. Smaller size ranges will have lower yields.
Cost is per sample. A normal cassette cartridge contains 4 sample lanes with 1 lane size reference. |
Internal
$122.00
each
External $147.00 each Corporate $203.00 each |
|
| Blue Pippin size selection 18kb - 27kb |
Size select samples using the Blue Pippin 0.75% agarose cassette, between 18 kb and 27 kb. Smaller size ranges will have lower yields.
Cost is per sample. A normal cassette cartridge contains 4 sample lanes with 1 size reference lane. |
Internal
$122.00
each
External $147.00 each Corporate $203.00 each |
|
| ► Shearing (2) | |||
| Name | Description | Price | |
| Covaris M220 Shearing, SnapCap |
Shear in Snap-Cap Covaris tubes with pre-set shearing protocols.
Client gives GRC 130uL of DNA to shear and receives sheared product in shearing tube.
Max 5ug/tube.
|
Internal
$15.00
each
External $18.00 each Corporate $25.00 each |
|
| Covaris M220 Shearing, ScrewCap |
Shear in Screw-Cap Covaris tubes with pre-set shearing protocols.
Client gives GRC 50uL of DNA to shear and receives sheared product in shearing tube.
|
Internal
$16.00
each
External $20.00 each Corporate $27.00 each |
|
| ► Consumables and Reagents (14) | |||
| Name | Description | Price | |
| Magnetic Beads, 1mL |
MagBio, sold ideally in 4mL aliquots
|
Internal
$9.00
each
External $11.00 each Corporate $15.00 each |
|
| Plate of amplicon primer/Illumina double barcoded adapters |
Primer pairs for second-round PCR of amplicon sequences (48 unique pairings, PCR-ready, 2uM). |
Internal
$34.00
each
External $41.00 each Corporate $57.00 each |
|
| Rainin LTS P10-20 Filtered Tips (960/unit) |
1case; 960tips |
Internal
$58.66
each
External $71.00 each Corporate $98.00 each |
|
| Rainin Filtered LTS P200 filtered tips (960/unit) |
1case; 960tips |
Internal
$58.66
each
External $71.00 each Corporate $98.00 each |
|
| Rainin Filtered LTS P1000 Filtered tips (768/unit) |
1case; 768tips |
Internal
$58.66
each
External $71.00 each Corporate $98.00 each |
|
| KingFisher 96well Tip-Comb, Fisher# 4388487 |
Internal
$6.50
each
External $8.00 each Corporate $11.00 each |
||
| KingFisher 96well Deep Plates, 4388476 |
Internal
$8.50
each
External $11.00 each Corporate $15.00 each |
||
| KingFisher 96well Standard Plates, 4388475 |
Internal
$4.17
each
External $6.00 each Corporate $7.00 each |
||
| Custom Sequencing Primer for 2step PCR on Illumina |
Enough custom-seuencing primer for a single MiSeq run |
Internal
$11.20
each
External $14.00 each Corporate $19.00 each |
|
| 24 wells of Nextera DNA/XT compatible dual-barcoded primers |
- ready to use (RTU) |
Internal
$295.00
each
External $354.00 each Corporate $491.00 each |
|
| 48 wells of Nextera DNA/XT compatible dual-barcoded primers |
- ready to use (RTU) |
Internal
$585.00
each
External $702.00 each Corporate $973.00 each |
|
| 72 wells of Nextera DNA/XT compatible dual-barcoded primers |
- ready to use (RTU) |
Internal
$804.00
each
External $965.00 each Corporate $1,338.00 each |
|
| 96 wells of Nextera DNA/XT compatible dual-barcoded primers |
- ready to use (RTU)
|
Internal
$1,059.00
each
External $1,271.00 each Corporate $1,762.00 each |
|
| BigDye |
$/uL |
Internal
$0.80
microliter
External $1.00 microliter Corporate $2.00 microliter |
|
| ► Bioinformatics (1) | |||
| Name | Description | Price | |
| Bioinformatics (Amplicon Cleaning/Processing for Community Analysis) |
An analysis pipeline designed to produce abundance tables for community analysis. The pipeline will take Illumina reads, process for quality and amplicon overlaps, and then assign reads to taxonomic groups according to the RDP classifier. Output will be abundance tables for each taxonomic level as precisely as possible.
|
Internal
$400.00
each
External $480.00 each Corporate $666.00 each |
|
| ► Training Fees (1) | |||
| Name | Description | Price | |
| Training Fee |
Training in Common Use Core Equipment (IRIC142). |
Internal
$50.00
/hr
External $60.00 /hr Corporate $84.00 /hr |
|
| ► Shipping (1) | |||
| Name | Description | Price | |
| Shipping Overnight FedEx on Dry-Ice |
One-time shipping charge for sending samples to outside facilities for further processing. |
Internal
$174.00
each
External $209.00 each Corporate $290.00 each |
|
| ► Hourly Services (4) | |||
| Name | Description | Price | |
| Bioinformatics (Custom) |
Working closely with the bioinformatics scientists within the core, users will determine to what degree their projects require bioinformatics assistance. Charges are per hour of work. |
Internal
$110.00
/hr
External $132.00 /hr Corporate $216.00 /hr |
|
| Consulting |
Consulting fee charged hourly |
Internal
$51.00
/hr
External $62.00 /hr Corporate $85.00 /hr |
|
| Preliminary Project Meeting (Free) |
Meeting with GRC to discuss a new project, 1hour |
Internal
$0.00
/hr
External $0.00 /hr Corporate $0.00 /hr |
|
| Custom Lab Protocols |
User must coordinate with the Core to determine the best approach for custom labwork.
|
Internal
$50.00
/hr
External $60.00 /hr Corporate $99.00 /hr |
|
| ► PacBio, DNA Isolation (4) | |||
| Name | Description | Price | |
| 1x Test HMW DNA Isolation |
Input: Intact/living/flash-frozen sample |
Internal
$478.00
each
External $574.00 each Corporate $795.00 each |
|
| 2x Test HMW DNA Isolation |
Input: Intact/living/flash-frozen sample |
Internal
$594.00
each
External $713.00 each Corporate $988.00 each |
|
| 3x Test HMW DNA Isolation |
Input: Intact/living/flash-frozen sample |
Internal
$709.00
each
External $851.00 each Corporate $1,180.00 each |
|
| 4x Test HMW DNA Isolation |
Input: Intact/living/flash-frozen sample |
Internal
$824.00
each
External $989.00 each Corporate $1,371.00 each |
|
| ► PacBio, analysis (1) | |||
| Name | Description | Price | |
| CCS processing |
Input: Primary-analysis files from SMRTlink (default paramaters unless otherwise specified) |
Internal
$120.00
each
External $144.00 each Corporate $200.00 each |
|
| ► PacBio, DNA library-prep (13) | |||
| Name | Description | Price | |
| 1x HiFi Library Template Prep Kit 3.0 |
Input: QC'ed DNA of interest ready to shear (up to 16ug, inquire if you have less than 14ug) |
Internal
$1,446.00
each
External $1,736.00 each Corporate $2,405.00 each |
|
| 2x HiFi Library Template Prep Kit 3.0 |
Input: QC'ed DNA of interest ready to shear (up to 16ug, inquire if you have less than 14ug) |
Internal
$2,173.00
each
External $2,608.00 each Corporate $3,614.00 each |
|
| 3x HiFi Library Template Prep Kit 3.0 |
Input: QC'ed DNA of interest ready to shear (up to 16ug, inquire if you have less than 14ug) |
Internal
$2,923.00
each
External $3,508.00 each Corporate $4,861.00 each |
|
| 4x HiFi Library Template Prep Kit 3.0 |
Input: QC'ed DNA of interest ready to shear (up to 16ug, inquire if you have less than 14ug) |
Internal
$3,661.00
each
External $4,394.00 each Corporate $6,089.00 each |
|
| 1x HiFi Amplicon Library Using Express v3.0 kit |
Input: barcoded amplicon of interest |
Internal
$1,106.00
each
External $1,328.00 each Corporate $1,840.00 each |
|
| 2x HiFi Amplicon Library Using Express v3.0 kit |
Input: barcoded amplicon of interest |
Internal
$1,618.00
each
External $1,942.00 each Corporate $2,691.00 each |
|
| 3x HiFi Amplicon Library Using Express v3.0 kit |
Input: barcoded amplicon of interest |
Internal
$2,154.00
each
External $2,585.00 each Corporate $3,583.00 each |
|
| 4x HiFi Amplicon Library Using Express v3.0 kit |
Input: barcoded amplicon of interest |
Internal
$2,678.00
each
External $3,214.00 each Corporate $4,454.00 each |
|
| 5x HiFi Amplicon Library Using Express v3.0 kit |
Input: barcoded amplicon of interest |
Internal
$3,202.00
each
External $3,843.00 each Corporate $5,325.00 each |
|
| 1x CLR Library using Express V3.0 |
Input: QC'ed DNA of interest ready to shear (1,000ng to 10,000ng; inquire if you have less as low-input solutions exist) |
Internal
$1,162.00
each
External $1,395.00 each Corporate $1,933.00 each |
|
| 2x CLR Library using Express V3.0 |
Input: QC'ed DNA of interest ready to shear (1,000ng to 10,000ng; inquire if you have less as low-input solutions exist) |
Internal
$1,766.00
each
External $2,120.00 each Corporate $2,937.00 each |
|
| 3x CLR Library using Express V3.0 |
Input: QC'ed DNA of interest ready to shear (1,000ng to 10,000ng; inquire if you have less as low-input solutions exist) |
Internal
$2,370.00
each
External $2,844.00 each Corporate $3,942.00 each |
|
| 4x CLR Library using Express V3.0 |
Input: QC'ed DNA of interest ready to shear (1,000ng to 10,000ng; inquire if you have less as low-input solutions exist) |
Internal
$2,974.00
each
External $3,569.00 each Corporate $4,946.00 each |
|
| ► PacBio, RNA library-prep (9) | |||
| Name | Description | Price | |
| 1x RNA-to-amplified-cDNA reaction, (for Iso-Seq) |
Input: ribo-depleted or total-RNA of interest, >= 300ng |
Internal
$462.00
each
External $555.00 each Corporate $769.00 each |
|
| 2x RNA-to-amplified-cDNA reactions, (for Iso-Seq) |
Input: ribo-depleted or total-RNA of interest, >= 300ng cumulatively |
Internal
$879.00
each
External $1,055.00 each Corporate $1,462.00 each |
|
| 3x RNA-to-amplified-cDNA reactions, (for Iso-Seq) |
Input: ribo-depleted or total-RNA of interest, >= 300ng cumulatively |
Internal
$1,150.00
each
External $1,380.00 each Corporate $1,913.00 each |
|
| 4x RNA-to-amplified-cDNA reactions, (for Iso-Seq) |
Input: ribo-depleted or total-RNA of interest, >= 300ng cumulatively |
Internal
$1,420.00
each
External $1,704.00 each Corporate $2,362.00 each |
|
| 5x RNA-to-amplified-cDNA reactions, (for Iso-Seq) |
Input: ribo-depleted or total-RNA of interest, >= 300ng cumulatively |
Internal
$1,690.00
each
External $2,028.00 each Corporate $2,811.00 each |
|
| 1x Iso-Seq Library using Express v2.0 kit |
Input: pooled or individual amplified (ds) cDNA, 160-500ng |
Internal
$911.00
each
External $1,094.00 each Corporate $1,515.00 each |
|
| 2x Iso-Seq Libraries using Express v2.0 kit |
Input: pooled or individual amplified (ds) cDNA, 160-500ng/library |
Internal
$1,486.00
each
External $1,784.00 each Corporate $2,472.00 each |
|
| 3x Iso-Seq Libraries using Express v2.0 kit |
Input: pooled or individual amplified (ds) cDNA, 160-500ng/library |
Internal
$2,061.00
each
External $2,474.00 each Corporate $3,428.00 each |
|
| 4x Iso-Seq Libraries using Express v2.0 kit |
Input: pooled or individual amplified (ds) cDNA, 160-500ng/library |
Internal
$2,636.00
each
External $3,164.00 each Corporate $4,384.00 each |
|
| ► PacBio, sequencing (4) | |||
| Name | Description | Price | |
| 1x PacBio Sequel II SMRTcell (8M) |
Input: PacBio compatible library, ready to sequence |
Internal
$3,197.00
each
External $3,837.00 each Corporate $5,317.00 each |
|
| 2x PacBio Sequel II SMRTcell (8M) |
Input: PacBio compatible library, ready to sequence |
Internal
$4,704.00
each
External $5,645.00 each Corporate $7,823.00 each |
|
| 3x PacBio Sequel II SMRTcell (8M) |
Input: PacBio compatible library, ready to sequence |
Internal
$6,211.00
each
External $7,454.00 each Corporate $10,329.00 each |
|
| 4x PacBio Sequel II SMRTcell (8M) |
Input: PacBio compatible library, ready to sequence |
Internal
$7,722.00
each
External $9,267.00 each Corporate $12,842.00 each |
|
| ► PacBio, wet-lab support protocols (4) | |||
| Name | Description | Price | |
| LightBench size-selection, 1x lane |
Input: 25uL DNA of interest, mass varies by cardridge's % agarose and DNA distribution |
Internal
$95.00
each
External $114.00 each Corporate $158.00 each |
|
| LightBench size-selection, 2x lanes |
Input: 50uL DNA of interest, mass varies by cardridge's % agarose and DNA distribution |
Internal
$109.00
each
External $131.00 each Corporate $182.00 each |
|
| LightBench size-selection, 3x lanes |
Input: 75uL DNA of interest, mass varies by cardridge's % agarose and DNA distribution |
Internal
$124.00
each
External $149.00 each Corporate $207.00 each |
|
| LightBench size-selection, 4x lanes |
Input: 100uL DNA of interest, mass varies by cardridge's % agarose and DNA distribution |
Internal
$138.00
each
External $166.00 each Corporate $230.00 each |
|
| ► PacBio, Sequel-II External Library, prepared for sequencing (4) | |||
| Name | Description | Price | |
| 1x Client-Provided HiFi or CLR Library, prep for sequencing |
Input: Library of Interest shipped O/N via FedEx, enough to sequence |
Internal
$644.00
each
External $773.00 each Corporate $1,071.00 each |
|
| 2x Client Provided HiFi or CLR Library, prep for sequencing |
Input: Library of Interest shipped O/N via FedEx, enough to sequence |
Internal
$879.00
each
External $1,055.00 each Corporate $1,462.00 each |
|
| 3x Client-Provided HiFi or CLR Library, prep for sequencing |
Input: Library of Interest shipped O/N via FedEx, enough to sequence |
Internal
$1,115.00
each
External $1,338.00 each Corporate $1,855.00 each |
|
| 4x Client-Provided HiFi or CLR Library, prep for sequencing |
Input: Library of Interest shipped O/N via FedEx, enough to sequence |
Internal
$1,350.00
each
External $1,620.00 each Corporate $2,246.00 each |
|
| ► unclassified (1) | |||
| Name | Description | Price | |
| Stranded Illumina RNA library, by hand |
Stranded RNA library made by had from RNA of interest. |
Inquire | |
