NMR and Drug Metabolism

 

Overview of Services

 

Welcome to the NMR and Drug Metabolism Core at Baylor College of Medicine.

 

The NMR and Drug Metabolism Core offers tools to support the discovery, synthesis, screening, optimization, metabolism and pharmacokinetics of small molecule ligands or lead compounds. Our 600 and 800 MHz Bruker nuclear magnetic resonance (NMR) spectrometers are available for unassisted use by trained users, and user training in simple 1D and 2D NMR data acquisition and analysis is also available. Expert services are available to determine small molecule identity or conformation, to identify and quantify metabolites, to determine macromolecular structure or detect perturbations upon ligand binding, or to screen compound libraries against purified targets. DNA-encoded technology (DEC-Tec) allows affinity-based selection of millions of different molecules in a single tube to identify those that most tightly bind a tagged, purified protein. We investigate the metabolism, metabolic stability, and pharmacokinetics of small molecular weight compounds using liquid chromatography-mass spectrometry (LC-MS and MS/MS). In addition to analyzing biological fluids or tissues, we assay stability in liver microsomes and perform reaction phenotyping against an array of CYP450s. Core personnel provide advice on the use of the supported methods for a wide variety of applications and assistance is available in project experimental design and data analysis.

 

Services and Resources:

• Equipment/Instrumentation                                                                                                                                                             
• Avance III HD 600 MHz spectrometer
• Avance III HD 800 MHz spectrometer
• Thermo Fisher Q Exactive
• Agilent 6490 triple quadrupole mass spectrometer (QQQ MS)

• Services
• Unassisted NMR usage for trained users: please use the 'Schedule Equipment' tab to schedule 600 or 800 MHz NMR time
• NMR training: please use the 'Request Services' tab to initiate an Equipment Training Request
• Assisted NMR usage: please use the 'Request Services' tab to inquire and receive a quote for fee-for-service NMR data acquisition and analysis
• Drug metabolism and pharmacokinetics: please use the 'Request Services' tab to request a quote for Drug Metabolism service

 

 

Getting Started

 

• BCM Users:  Click the link in the upper right corner to login or register for an iLab account using valid BCM credentials.

• Non-BCM Users:  Click the appropriate link in the upper right corner to login with your approved iLab credentials or to sign up for an iLab account.

• Visit the Customer Help Manual for further guidance using iLab's Core Facility Services.

 

  

Leadership and Staff

 

Kevin MacKenzie, Ph.D. | Academic Director

Email:  Kevin.MacKenzie@bcm.edu

Phone: 832-824-8155

 

Name	Role	Phone	Email	Location
Feng Li	Co-Director	713-798-3623	Feng.Li@bcm.edu
Kimberly Kerlec	Operations Director	713-798-8132	Kerlec@bcm.edu	M904G
Hongbing Huang	DEC-Tec Lead	713-798-7693	Hongbing.Huang@bcm.edu
James Campbell	SPR Specialist	713-798-8687	Jccampbe@bcm.edu	N930
Gabriella Miklossy	DEC-Tec Specialist	713-798-2292	Gabriella.Miklossy@bcm.edu
Zhifeng Yu	DEC-Tec Specialist	713-798-8533	Zhifeng.Yu@bcm.edu
Murugesan Palaniappan	DEC-Tec Specialist	713-798-2036	palaniap@bcm.edu

 

 

Location and Hours of Operation

 

Location	Hours
NMR and Drug Metabolism Core Baylor College of Medicine Jan and Dan Duncan Neurological Research Institute 1250 Moursund Street Houston, TX 77030 2nd Floor - NMR 6th Floor - Mass Spectrometer Phone:  713-798-8132	7:30 a.m. - 5:30 p.m., Monday to Friday

 

Links and Resources

 

• Visit our BCM core website for additional information.

  

 

Publication Acknowledgements

 

Scientific publications containing data obtained using the services offered by the core should include the following statements.

In the methods section: The ____ (ADD YOUR SCREEN OR ASSAY) screens were performed in the NMR and Drug Metabolism Core at Baylor College of Medicine.

In the acknowledgments section: We appreciate the technical support, advice, and assistance of ______ (ADD THE INDIVIDUAL(S) THAT HELPED WITH THE SCREEN OR ASSAY) in the NMR and Drug Metabolism Core at Baylor College of Medicine.

Grants which support core operations include, CPRIT Core Facility Support Award (RP160805) and Welch Foundation Grant (H-Q-0042).