Laboratory Bioanalysis Biotechnology

Getting Started

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Overview of Services

The Laboratory Bioanalysis Biotechnolog (LBB)

The Laboratory Bioanalysis Biotechnolog (LBB) core provides DNA sequencing, protein sequencing, oligonucleotide synthesis, peptide synthesis, and some information services to members of the Center for Reproductive Biology. A state of the art core laboratory providing all genomics micro-array technology equipment including access to an Affymetrix Micro-Array apparatus and data analysis system, as well as a custom array apparatus for glass slide array of investigator initiated arrays. The Core has the bio-informatics support for the procedures performed and assists the investigators in data analysis. Technical support is provided to perform the analysis on the Affymetrix chips and in the production of the custom arrays.

Center for Reproductive Biology - Coming Soon

The WSU Center for Reproductive Biology is one of the largest reproductive biology centers in the world. The inter-institutional center has nearly 90 faculty members and more than 270 students, postdoctoral fellows, and technical staff from 26 departments across the WSU system and other top-tier institutions around the country.

The center provides opportunities for investigators to collaborate, learn from one another, and advance our understanding of reproductive biology, developmental biology, and associated diseases.

Gene Editing Reagent Core - Coming Soon

The mission of the Gene Editing Reagent Core (GERC) is to help investigators integrate cutting edge genome manipulation into their research. Founded in 2017 as part of the Functional Genomics Initiative, the goal of the GERC is to further WSU as a leader in genome editing in agriculture and to elevate the life sciences research across the institution and beyond. Using CRISPR/Cas, the ability to generate precise changes in the DNA of almost any species is achievable. While there are ever increasing numbers of potential applications, without expertise in genetic engineering, successfully incorporating genome editing in research efforts can be daunting. As a fee for service core resource, the GERC provides expertise and produces high quality reagents and animal models that investigators can readily apply into their downstream applications. The location of the GERC in the Center for Reproductive Biology allows a close collaboration with the WSU Animal Production Core (APC) which is optimal to developed gene edited animals. This relationship has allowed over 30 new animal models to be delivered to investigators.

The GERC also facilitates generation of single cell libraries through the 10xGenomics Chromium workflows. Investigators using this service will provide a validated single cell suspension and the GERC will return a ready to be sequenced single cell library.

 The Animal Production Core - Coming Soon

The Animal Production Core (APC) offers a range of services for generating and preserving gene-edited and genetically-modified animal models for investigators at WSU. Projects from investigators outside WSU are also welcome, and we are happy to discuss possible research collaborations.
Embryo microinjection and micromanipulation services are available for generation of transgenic, knock-out, knock-in, or otherwise genetically-engineered animals, using zygote or blastocyst injection with DNA, RNA, CRISPR-Cas9 reagents, or ES cells. We work in close collaboration with the Gene Editing Reagent Core (GERC) to provide full-service genome editing services, from design of targeting strategies to delivery of founder animals to investigators.
The Animal Production Core can provide a range of traditional transgenic core services, with experience and expertise in embryo transfer surgeries, embryo and sperm cryopreservation and cryorecovery, rederivation of specific-pathogen-free lines, in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), and other reproductive biology techniques. Well-established protocols are in place for working with mouse and rat embryos and gametes, and we are happy to work with other laboratory animal models and large animal species as well.

Fluorescent Activated Cell Sorting Core - Coming Soon

The core contains two FACS machines manufactured by Sony Biotechnology that contain 4 lasers each.  The SY3200 FACS machine is capable of 4 way color sorting into tubes or plates and the SH800 is capable of 2-way color sorting into tubes.  Both machines are available for use by the WSU community on a fee-for-service basis.

Location and Hours of Operation

Links and Resources

College of Veterinary Medicine

Center for Reproductive Biology

Animal Production Core

Fluorescent Activated Cell Sorting Core

Contacts

Map