High Content Screening Facility (La Jolla)

Getting Started

Internal SBPMDI

• If you have an iLab account, please login at the top right or use this link https://ilab.sbpdiscovery.org/
• If you do not have an iLab account, please click HERE to request an account. All fields are required.

 External SBPMDI

• You must have a Customer ID and iLab account to request services from this facility.
• To obtain a Customer ID please email Susanne Heynen-Genel at sheynen@sbpdiscovery.org
• To obtain an iLab account, please click HERE.

Forgot your password? Please click HERE.

Overview of Services

The High Content Screening (HCS) Facility supports image-based high content assays utilizing high throughput microscopy. The HCS team has expertise in all areas of high content image-based screens including sample preparation, image acquisition, image analysis, image data management, and algorithm development. The HCS facility also supports the screening efforts of the Functional Genomics Shared Resources, providing expertise for HCS assays using RNAi libraries. 

 Assay Conceptualization:

• Initial consultation for biological feasibility
• Aid in selection of reagents, cell lines, and assay conditions

Single-Plate Experiments & Small-Scale HCS (Plate Preparation by PI Lab Scientest):

• Use of HCS instruments including training
• Use of analysis software including training
• Develop and validate image analysis algorithms and assay read-outs

 Medium- and Large-Scale Screens (Plate Preparation by HCS or Functional Genomics Facility):

• Optimize assay biology
• Develop image analysis algorithms and assay read-outs
• Perform assay validation, miniaturization, and implementation
• Screen in collaboration with HTS or Functional Genomics facilities

 Dara Analysis and Image-data Management:

• Perform normalization, hit selection, and dose response
• Short-term storage of images and raw data
• Aid with images and data visualization

High Content Assays - Useful Links

Assay Guidance Manual: http://www.ncbi.nlm.nih.gov/books/NBK100913/

Nikon Microscopy: http://www.microscopyu.com

Zeiss Microscopy: http://zeiss-campus.magnet.fsu.edu/index.html

Olympus Microscopy: http://www.olympusmicro.com/primer/anatomy/anatomy.html

High Content Plate Recommendations 

Information on general plate selection for HCA / HCS can be found here: https://www.perkinelmer.com/lab-products-and-services/application-support-knowledgebase/microplates/high-content-screening-plates.html

Leadership

Susanne Heynen-Genel | Director

 Location and hours of operation

Contacts