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Welcome!
The Advanced Genome Technologies Core is an overarching umbrella encompassing three distinct shared resource facilities: The Vermont Cancer Center DNA Analysis Facility, the Vermont Genetic Network Microarray Facility, and the Vermont Cancer Center-College of Medicine Massively Parallel Sequencing Facility. The mission of the core is to comprehensively support research by providing direct services to research laboratories and by introducing emerging technologies to the broader University community. The facilities within the Advanced Genome Technologies Core offer a wide breadth of services to facilitate research involving identification, quantification, and profiling of nucleic acids.
Please use the following link to view our website: http://www.uvm.edu/medicine/vtcancercenter/?Page=facilities_agtc.html
|
Hours |
Location |
|
7:30am - 5 pm Mon-Fri. |
305 HSRF |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Jessica Hoffman |
DNA Analysis Facility
|
802-656-2557
|
jessica.hoffman@uvm.edu
|
305 HSRF
|
| Daniel Haupt |
Microarray Facility
|
802-656-2557
|
daniel.haupt@uvm.edu
|
305 HSRF
|
| Scott Tighe |
Massively Parallel Sequencing Facility
|
802-656-AGTC
|
scott.tighe@uvm.edu
|
303 HSRF
|
| Sample Submission and Laboratory Services |
| ► UVMCC DNA Analysis Facility (13) | |||
| Name | Description | Price | |
|---|---|---|---|
| Applied Support | Inquire | ||
| human or mouse Cell Line Authentication |
Includes gDNA extraction and Genescan Run. |
Inquire | |
| [Fragment Analysis] - Full 96 well Plate (per plate) | Inquire | ||
| [Fragment Analysis] - Genescan Run (per sample) | Inquire | ||
| [Gene Expression] - PCR Arrays | Inquire | ||
| [Gene Expression] - qPCR-Full Service | Inquire | ||
| [Image Analysis] - BioRad Image Scan | Inquire | ||
| [Nucleic Acid Extraction] - gDNA Extraction (per sample) | Inquire | ||
| [Nucleic Acid Extraction] - RNA-cells (per sample) | Inquire | ||
| [Nucleic Acid Extraction] - RNA-tissue and blood (per sample) | Inquire | ||
| [Sequencing] - Bulk Sequence (min. 48 samples) |
A minimum of 48 samples is required for Bulk Sequence Requests. Submissions MUST be in a 96 well plate and the user is required to combine the template, primer and water. |
Inquire | |
| [Sequencing] - Cycle Sequence Rxn and Sequence Run |
For this request, please submit template and primer and the facility will set up the cycle sequence reaction and complete the sequence run. |
Inquire | |
| [Sequencing] - Sequence Run Only |
User must complete Cycle Sequencing Reaction and clean the Cycle Sequence products before bringing to the facility. |
Inquire | |
| ► VCC-COM Massively Parallel Sequencing Facility (16) | |||
| Name | Description | Price | |
| ChIP-Seq DNA Library Preparation |
Have you included an "Input" and "IgG control"? |
Inquire | |
| DNA Library Preparation-Nextgen Sequencing | Inquire | ||
| Exome Capture |
By Quote only. |
Inquire | |
| NGS Library Quantitation qPCR (KAPA) | Inquire | ||
| NGS Library Quantitation qPCR and Qubit | Inquire | ||
| Oxford Nanopore Sequencing Library and Sequencng |
Oxford Nanopore Sequencing must be approval by facility staff prior to sample submission. General Q-Score average 8-11 on the MK1 R7 Porecell |
UVM Internal
$950.00
each
External $1,425.00 each Vt State Institutions $950.00 each |
|
| RNA Library Preparation-Eukaryote and Prokaryote (includes ribo-reduction) | Inquire | ||
| RNA Library Preparation-Eukaryotic and Prokaryote |
This price does not include Ribosomal reduction step. |
Inquire | |
| Self Generated MPS Libraries Sequencing PE 2x100BP (Paired End) | Inquire | ||
| Self Generated MPS Libraries Sequencing PE50BP (Paired End) | Inquire | ||
| Self Generated MPS Libraries Sequencing SE100BP (Single End) | Inquire | ||
| Sequencer Prep-Hyb Cocktail Prep for User generated MPS Libraries |
For final assembly of User generated Sequencing libraries. Included is Qubit, HT1 hyb prep, qPCR with KAPA, dilutions and PhiX spike in as needed |
UVM Internal
$171.30
each
External $317.10 each |
|
| Sequencing-by-Synthesis-100 bp Paired End (200bp) | Inquire | ||
| Sequencing-by-Synthesis: 100bp Single End | Inquire | ||
| Sequencing-by-Synthesis: 50 bp Single End | Inquire | ||
| Small RNA Library Prep | Inquire | ||
