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Welcome!
The Advanced Genome Technologies Core is an overarching umbrella encompassing three distinct shared resource facilities: The Vermont Cancer Center DNA Analysis Facility, the Vermont Genetic Network Microarray Facility, and the Vermont Cancer Center-College of Medicine Massively Parallel Sequencing Facility. The mission of the core is to comprehensively support research by providing direct services to research laboratories and by introducing emerging technologies to the broader University community. The facilities within the Advanced Genome Technologies Core offer a wide breadth of services to facilitate research involving identification, quantification, and profiling of nucleic acids.
Please use the following link to view our website: http://www.uvm.edu/medicine/vtcancercenter/?Page=facilities_agtc.html
Advanced Genome Technologies Core
Tim Hunter - Core Director
Phone: 802-656-2559 (office)
Email: timothy.hunter@uvm.edu
|
Hours |
Location |
|
7:30am - 5 pm Mon-Fri. |
305 HSRF |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Tim Hunter |
Core Director, Advanced Genome Technologies Core
|
802-656-2559
|
timothy.hunter@uvm.edu
|
306 HSRF
|
| Jessica Hoffman |
Research Technician, VCC DNA Analysis Facility
|
802-656-2557
|
jessica.hoffman@uvm.edu
|
305 HSRF
|
| Nelson Vila-Santana |
Research Technician, VGN Microarray Facility
|
802-656-3936
|
nelson.vila-santana@uvm.edu
|
305 HSRF
|
| Scott Tighe |
Senior Research Technician, Massively Parallel Sequencing Facility
|
802-656-AGTC
|
scott.tighe@uvm.edu
|
303 HSRF
|
| Sample Submission and Laboratory Services |
| ► VCC DNA Analysis Facility (13) | |||
| Name | Description | Price | |
|---|---|---|---|
| Applied Support | Inquire | ||
| human or mouse Cell Line Authentication |
Includes gDNA extraction and Genescan Run. |
Inquire | |
| [Fragment Analysis] - Full 96 well Plate (per plate) | Inquire | ||
| [Fragment Analysis] - Genescan Run (per sample) | Inquire | ||
| [Gene Expression] - PCR Arrays | Inquire | ||
| [Gene Expression] - qPCR-Full Service | Inquire | ||
| [Image Analysis] - BioRad Image Scan | Inquire | ||
| [Nucleic Acid Extraction] - gDNA Extraction (per sample) | Inquire | ||
| [Nucleic Acid Extraction] - RNA-cells (per sample) | Inquire | ||
| [Nucleic Acid Extraction] - RNA-tissue and blood (per sample) | Inquire | ||
| [Sequencing] - Bulk Sequence (min. 48 samples) |
A minimum of 48 samples is required for Bulk Sequence Requests. Submissions MUST be in a 96 well plate and the user is required to combine the template, primer and water. |
Inquire | |
| [Sequencing] - Cycle Sequence Rxn and Sequence Run |
For this request, please submit template and primer and the facility will set up the cycle sequence reaction and complete the sequence run. |
Inquire | |
| [Sequencing] - Sequence Run Only |
User must complete Cycle Sequencing Reaction and clean the Cycle Sequence products before bringing to the facility. |
Inquire | |
| ► VCC-COM Massively Parallel Sequencing Facility (16) | |||
| Name | Description | Price | |
| ChIP-Seq DNA Library Preparation |
Have you included an "Input" and "IgG control"? |
Inquire | |
| DNA Library Preparation-Nextgen Sequencing | Inquire | ||
| Exome Capture |
By Quote only. |
Inquire | |
| NGS Library Quantitation qPCR (KAPA) | Inquire | ||
| NGS Library Quantitation qPCR and Qubit | Inquire | ||
| Oxford Nanopore Sequencing Library and Sequencng |
Oxford Nanopore Sequencing must be approval by facility staff prior to sample submission. General Q-Score average 8-11 on the MK1 R7 Porecell |
UVM Internal
$950.00
each
External $1,425.00 each Vt State Institutions $950.00 each |
|
| RNA Library Preparation-Eukaryote and Prokaryote (includes ribo-reduction) | Inquire | ||
| RNA Library Preparation-Eukaryotic and Prokaryote |
This price does not include Ribosomal reduction step. |
Inquire | |
| Self Generated MPS Libraries Sequencing PE 2x100BP (Paired End) | Inquire | ||
| Self Generated MPS Libraries Sequencing PE50BP (Paired End) | Inquire | ||
| Self Generated MPS Libraries Sequencing SE100BP (Single End) | Inquire | ||
| Sequencer Prep-Hyb Cocktail Prep for User generated MPS Libraries |
For final assembly of User generated Sequencing libraries. Included is Qubit, HT1 hyb prep, qPCR with KAPA, dilutions and PhiX spike in as needed |
UVM Internal
$171.30
each
External $317.10 each |
|
| Sequencing-by-Synthesis-100 bp Paired End (200bp) | Inquire | ||
| Sequencing-by-Synthesis: 100bp Single End | Inquire | ||
| Sequencing-by-Synthesis: 50 bp Single End | Inquire | ||
| Small RNA Library Prep | Inquire | ||
| ► VGN Microarray Facility (11) | |||
| Name | Description | Price | |
| Bacterial 16S PCR Amplification | Inquire | ||
| ChIP on Chip including Hyb/scan | Inquire | ||
| DNA 1000 Quality Assessment (up to 12 Samples/chip) | Inquire | ||
| GeneChip Hybridization and Scan (Microarray Target prep with USER PURCHASED reagents) |
Turnaround time: 5-10 business days |
Inquire | |
| miRNA Target Prep including Hyb/Scan |
Turnaround time: 5-10 business days |
Inquire | |
| Phylochip Target Prep including Hyb/Scan |
Turnaround time: 5-10 business days. |
Inquire | |
| Qubit Quantification | Inquire | ||
| SNP / Mapping Target Prep 500K (Nsp or Sty) including Hyb/Scan |
Turnaround time: 5-10 business days |
Inquire | |
| SNP/Mapping 250K (Nsp or Sty) Target Prep (Hyb/Scan with reduced price CORE Arrays) | Inquire | ||
| Total RNA Quality Assessment (1-12 samples) | Inquire | ||
| WT Nugen Pico Ovation Target Prep including Hyb/Scan |
Turnaround time: 5-10 business days |
Inquire | |
