Biomolecular Core Laboratory

 

Nemours Biomolecular Core Facility (RRID:SCR_018265)

Research Resource Identifiers (#RRID) are ID numbers assigned to help researchers cite key resources (antibodies, model organisms and software projects) in the biomedical literature to improve transparency of research methods. RRIDs are part of the MDAR checklist, a pan-publisher rigor and transparency checklist created and endorsed by major publishers. RRIDs are also part of the NISO JATS standard, the journal article tagging suite.

Please include this citation in any publication of data generated by the BCL:

Nemours Biomolecular Core Facility (RRID:SCR_018265)

 

Overview of Services

The Biomolecular Core Lab (BCL) provides essential services in molecular biology and genetics to the clinical and research staff at Nemours, Delaware Institutional Development Award/IDeA Networks of Biomedical Research Excellence (INBRE) partner institutions, and external clients. The services the core offers include: DNA Sanger Sequencing, DNA Fragment Analysis, SNP analysis, Nucleic Acid isolation, Cell Identification/Authentication,  Gene Expression analysis, Digital PCR, Microarray assays, Custom Oligonucleotide Synthesis, Robotic liquid handling procedures, Microplasma testing, and Fragment Analyzer technology.

Preparative instrumentation available for research staff to use (i.e., after training) in the core includes: QS 7 FLEX Real-Time PCR Instrument, Biomek 4000 & 3000 liquid handlers, NanoDrop UV-Vis spectrophotometer, PCR thermal cyclers, Covaris focused-ultrasonicator , and Qubit fluorometer. Other instrumentation is provided as a staffed service. Support services include design consultation, data troubleshooting, and instrument training. The ultimate goal of this laboratory is to provide biomedical investigators with state of the art technical support and clinicians with tools that can help improve pediatric supportive care and/or therapeutic intervention.

We offer low-cost, fast-turnaround, high-quality service that are essential to many clinical and hypothesis driven projects and we operate under strict CLIA guidelines. 

Customers come to the core to access expertise, experience and of course, specialized state-of-the-arts equipment. We consider it paramount to stay at the cutting edge through active participation with Core Networks at the local, regional, national and international level.

  • Local: BCL shares activities with local network cores (Bioinformatics, Microarray, Bioimaging, Protein production, Structural biology-NMR, Mass Spec-proteomics, Genotyping and DNA sequencing) by participating in regular Core Director meetings, open houses and resource sharing.
  • Regional/National: BCL is funded in part by a COBRE (center of biomedical research excellence) grant, and participates in the regional and annual meetings of NIH-funded COBREs and INBREs (IDeA Network of Biomedical Research Excellence).
  • International: The BCL Staff are members of the ABRF (Association of Biomolecular Resource Facilities) and active participants in this professional association's annual meetings.

Leadership

 

 

Jennifer Holbrook | Program Manager Nemours Biobank and Molecular Analysis Program

Ph: (302)-651-6824

Ph: (302)-651-6712 (lab)

 

Deborah L. Stabley | Research Associate

Ph: (302) 651-6712

 

Location and hours of operation

 

Hours Location

Monday - Friday

8:00am - 5:00pm

Excluding Holidays         

Biomolecular Core Laboratory

1701 Rockland Road

Rockland Center 1, room 214

Wilmington, DE 19803

Links and Resources

 

  1. Nemours Biomolecular Core Laboratory Website: http://nemoursresearch.org/cores/bcl
  2. Childrens Health System Nemours Website: http://www.nemours.org
  3. BCL iLabSolutions FAQv1

Contacts

Name Role Phone Email Location
Jennifer Holbrook
Core Manager
 
302-651-6824 Fax: 302-651-5176
 
mbcore@nemours.org
 
RC1 room 235