Biomolecular Core Laboratory

Announcements

 

 

 

Overview of Services

The BCL staff offers support in Sanger DNA sequencing, DNA fragment analysis, and SNP analysis; Ion PGM NGS; Real-Time PCR; 3D PCR; microarray; custom oligonucleotide synthesis; robotic liquid handling methodologies; and bioanalyzer "lab-on-a-chip" technology.

Since its inception in 2001, BCL has developed a competitive research project in pediatric genetics and provided essential services in molecular biology and genetics to clinicians and research scientists at Nemours and affiliated partner institutions including University of Delaware, Thomas Jefferson College of Medicine, Delaware State University, Wesley College, Delaware Technical Community College and Christiana Care Health System. 

 We offer low-cost, fast-turnaround, high-quality service that are essential to many clinical and hypothesis driven projects and we operate under strict CLIA guidelines. 

We are a fee for service core but provide free consultations on project design, and data troubleshooting, Support is also available for grant applications and preparing budgets.  BCL is a collaborative research lab providing support for the development of new research projects and training opportunities to  staff and students of all ages.

 

 

Customers come to the core to access expertise, experience and of course, specialized state-of-the-arts equipment. We consider it paramount to stay at the cutting edge through active participation with Core Networks at the local, regional, national and international level.

  • Local: BCL shares activities with local network cores (Bioinformatics, Microarray, Bioimaging, Protein production, Structural biology-NMR, Mass Spec-proteomics, Genotyping and DNA sequencing) by participating in regular Core Director meetings, open houses and resource sharing.
  • Regional/National: BCL is funded in part by a COBRE (center of biomedical research excellence) grant, and participates in the regional and annual meetings of NIH-funded COBREs and INBREs (IDeA Network of Biomedical Research Excellence).
  • International: The BCL Staff are members of the ABRF (Association of Biomolecular Resource Facilities) and active participants in this professional association's annual meetings.

Leadership

Jennifer Holbrook | Assistant Director / Research Associate

Ph: (302)-651-6824

Ph: (302)-651-6712 (lab)

 

Deborah L. Stabley | Biotechnology Specialist / Research Associate

Ph: (302) 651-6712

 

Location and hours of operation

 

Hours Location

Monday - Friday

8:00am - 5:00pm

Excluding Holidays         

Biomolecular Core Laboratory

1701 Rockland Road

Rockland Center 1, room 214

Wilmington, DE 19803

Links and Resources

 

  1. Nemours Biomolecular Core Laboratory Website: http://nemoursresearch.org/cores/bcl
  2. Childrens Health System Nemours Website: http://www.nemours.org
  3. BCL iLabSolutions FAQv1

Contacts

Name Role Phone Email Location
Jennifer Holbrook
Core Manager
 
302-651-6824 Fax: 302-651-6767
 
mbcore@nemours.org
 
RC1 room 229
 

Services

Name Description
Budget Preparation 

Please fill out this form in order for the BCL to prepare a budget for your project/proposal.

 
Covaris M220 Shearing 

Shearing of DNA, RNA or Protein using the Covaris M220 Adaptive Focused Acoustic Ultrasonicator.

 
DNA Fragment Analysis 

Citation Information:

The Biomolecular Core Lab is supported by grants P30GM114736 (COBRE) and P20GM103446 (INBRE). Please include these citations in any publication of data generated by the BCL.

 

Run only fragment analysis on ABI 3130xl. Investigator provides sample already processed and cleaned up. For fragments 100-500 base pairs.

 

Please see Fragment Analysis Protocol.pdf

 
DNA Isolation 

Perform DNA isolation via Gentra PureGene kit from blood/tissue/cells. Charge is per sample. DNA QC via Nanodrop included. 

 
DNA Sanger Sequencing 

Citation Information:

The Biomolecular Core Lab is supported by grants P30GM114736 (COBRE) and P20GM103446 (INBRE). Please include these citations in any publication of data generated by the BCL.

 

Run only sanger sequencing on ABI 3130xl Genetic Analyzer. Investigator performs sanger sequencing reaction and supplies cleanup sample to core for run on instrument.

 

For considerations when cleaning up plasmid preps see Sequencing Contaminants.pdf

 

For Sequencing Protocals please see link below:

 
Experimental Design/Consultation 

Consultation with the Director or Assistant Director on experiment design, researching technologies or services.

 
Identification and Authentication of Human Cell Lines and Specimens 

Most journals are making cell line authentication a pre-requisite for publication.

In addition, funding agencies are making cell line authentication a requirement for grant support.

 

DNA from human cell lines and specimens (including FFPE, tissue biopsies, DNA bank, blood, buccal cells, etc) are profiled using a STR (short tandem repeat) assay. Will report on gender; confirm presence/absence of contamination, and confirm identity of specimen with previous known STR profiles.

 

DNA submission - minimum of 20ul @ 20ng/ul in 1.5ml Eppendorf tubes. Use of a quantitative method that reads double stranded DNA (such as Qubit or picogreen) is highly recommended. Label your submission tube with your initials and a number (i.e. JH01, JH02, etc).  Additional sample name information can be added in your submission sheet.

 

DNA isolation is available as a separate service. See "DNA/RNA Isolation" section for details and to submit a request.

 

 
Ion PGM Library Prep 

Citation Information:

The Biomolecular Core Lab is supported by grants P30GM114736 (COBRE) and P20GM103446 (INBRE). Please include these citations in any publication of data generated by the BCL

 

Library preparation for the Ion Torrent PGM system. Includes Library QC. The final cost of the Library Prep is project specific. BCL will provide a quote.

 

 
Ion PGM Sequencing - 16S Metagenomics 

Citation Information:

The Biomolecular Core Lab is supported by grants P30GM114736 (COBRE) and P20GM103446 (INBRE). Please include these citations in any publication of data generated by the BCL

 

Ion PGM panel sequencing using the HiQ chemistry, 400 bp sequencing, for the 16S Metagenomics panel. Does not include library prep.

 

 
Letter of Support 

Letter of support for BCL core lab Director.

 
Pippin Size Selection Service 

Size selection of restriction or PCR products and sheared genomic DNA using the Pippin Prep

 
Quant Studio 3D Digital PCR 

Citation Information:

The Biomolecular Core Lab is supported by grants P30GM114736 (COBRE) and P20GM103446 (INBRE). Please include these citations in any publication of data generated by the BCL

 
RNA Isolation 

Isolate RNA from starting material, blood, tissue, cells. Charges are Per Sample. RNA QC via Nanodrop is included. 

 

 
SNP Genotyping 

SNP Genotyping on the 7900HT Fast Real-Time PCR system. Assay cost is not included. 

 

Members at The Delaware Vermont iLab Network (DEVIN)

Delaware State University

Core Name Primary Contact Email Phone Number/Ext
Oscar Imaging Facility Michael Moore mmoore@desu.edu 302-857-7035

Nemours

Core Name Primary Contact Email Phone Number/Ext
Cell Science Core Lisa Glazewski, MS CellScienceCore@Nemours.org (302) 651-6707
HTS and Drug Discovery Sigrid Langhans langhans@nemoursresearch.org (302) 651-6538
Healthcare Delivery Science Jennifer Christofferson jennifer.christofferson@nemours.org 302-298-7876
Histochemistry and Tissue Processing Core Histochemistry and Tissue Processing Core 1600 Rockland Road, Wilmington DE 19803
Translational Neuroimaging Core Shaohui (Shao) Zhang, PhD Shaohui.Zhang@nemours.org 302-651-6869
Biomolecular Core Laboratory Jennifer Holbrook mbcore@nemours.org 302-651-6824 Fax: 302-651-6767

University of Delaware

Core Name Primary Contact Email Phone Number/Ext
University of Delaware DNA Sequencing & Genotyping Center

Bruce Kingham brucek@udel.edu (302) 831-0823
Core Name Primary Contact Email Phone Number/Ext
Bioinformatics Core

Dr. Bryan Ballif bryan.ballif@uvm.edu (802) 656-1389
Proteomics Facility

Dr. Ying Wai Lam Ying-Wai.Lam@uvm.edu (802) 656-4709