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Cancer Metabolism is a Shared Resource within the Sanford Burnham Prebys Cancer Center. The role of the core is to support labs in the study of metabolism in cancer by (1) providing expertise in design of metabolic experiments, and interpretation of results; (2) providing analytical services for metabolism research, based on three platforms: (A) YSI analyzer for measurement of glucose, lactate and glutamine in culture medium; (B) Seahorse XFp for real-time measurement of oxygen consumption and extracellular acidification; (C) GC-MS quantification of small polar metabolites (TCA cycle, amino acids), sugars, fatty acids, or short-chain fatty acids. We also have expertise and offer service in stable isotope (13C) labeling tracing by GC-MS. We have experience working with cultured cells, tissue and tumor samples, and serum or plasma. We provide detailed protocols for sample preparation, and have worked with numerous researchers at various institutions on cancer and non-cancer metabolism projects.
David Scott, Ph.D. | Director
| Hours | Location |
|
9 am - 6 pm |
Building 10 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| David Scott, Ph.D. |
Director
|
dscott@sbpdiscovery.org
|
||
| Services Price List |
| ► Services (21) | |||
| Name | Description | Price | |
|---|---|---|---|
| Cholesterol/Sterol Quantification (per sample) | Inquire | ||
| Combined 13C and quantitative analysis for polar metabolites | Inquire | ||
| Combined Fatty Acid Quantification and 13C analysis (per sample) | Inquire | ||
| Fatty acid 13C analysis | Inquire | ||
| Fatty Acid and Cholesterol Quantification (per sample) | Inquire | ||
| Miscellaneous Instrument Use | Inquire | ||
| Polar 13C Analysis (per sample) | Inquire | ||
| Polar Metabolite Quantification (cells/ medium, per sample) | Inquire | ||
| Polar Metabolite Quantification (serum/ tissue), per sample) | Inquire | ||
| Polar Metabolite Quantification (total metabolite/ custom assays, per sample) | Inquire | ||
| Sample prep - cells or cell extracts, medium, plasma or serum | Inquire | ||
| Sample prep - homogenizer: fecal or cecum; weighed tumor or tissue | Inquire | ||
| Sample prep - homogenizer: tumor, tissue - not weighed/ needs re-weighing | Inquire | ||
| Sample prep - urine +urease | Inquire | ||
| Sample Prep and Data Collection (per sample) | Inquire | ||
| Seahorse XFp assay (per plate) | Inquire | ||
| Seahorse XFp assay (self-service, per plate) |
Self-service rate available after initial training at full-service rate, for SBP users |
Inquire | |
| Short-chain fatty acid analysis | Inquire | ||
| Sugars GCMS analysis | Inquire | ||
| Tumor or tissue GC-MS analysis (per sample) |
This price code covers either stable isotope tracing or metabolite quantification by GC-MS for tumor or tissue samples |
Inquire | |
| YSI metabolite analysis (per plate) | Inquire | ||
