BCH IDDRC Molecular Genetics Core Facility

NanoString nCounter® MAX Profiler:  A simple, cost effective solution for multiplexing 10 – 800 targets in a single tube.

• Upgraded from the nCounter Sprint to the nCounter Max!
• Eliminate the need for cDNA synthesis, PCR or library prep by using this simple hybridization technology. 
• Assay DNA, RNA, miRNA or protein in 24 hours without the need for technical replicates. 
• Flexible sample input allows you to use purified RNA, cell lysate, blood, FFPE.
• Easy data analysis done at your desk with free software(nSolver software) for quick, publication quality figures.
• Pre-designed panels or custom options available through Nanostring.  For assistance, please contact Brendan Collins at <bcollins@nanostring.com>.
  

Analysis of Bionano Saphyr data.  Price is per sample.  Deliverable is a research report.

Please note that the MGCF is NOT a CLIA facility.

This instrument is self service. Please request under the Equipment Reservations tab. 

The CFX384 Touch Real-Time PCR Detection System is a powerful and precise real-time PCR instrument in a 384-well format, for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System utilizes accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions.

Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR.

Key Features and Benefits

With the CFX384 Touch Real-Time PCR Detection System you can:

• Obtain precise quantification and multiplex target discrimination – optical system uses long-lasting solid-state technology with 5 filtered LEDs and 5 filtered photodiodes
• Confirm system performance and data reliability – internal system qualification and system test software, automated data quality control, and various validation tool services are available.
• Obtain accurate results with volumes as low as 3 µl – minimize sample and reagent usage
• Use advanced data analysis tools — normalized gene expression using CFX Maestro Software
• CFX Maestro Software Works Seamlessly with PrimePCR™ Assays and Panels. The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.
• Begin analyzing data in a single step by importing target, reference, and control information from a PrimePCR run file directly into CFX Maestro Software
• Combine data from multiple plates into a Gene Study to rapidly screen large numbers of targets or samples
• Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation

Assistance with data movement and transfer.  1 hour minimum.

Pricing listed is per 8 sample (column based). Please enter per column.

Investigator provides assay and restriction enzyme if necessary.

***Please clearly specify how materials need to be stored***

Please inquire for details and specifications.

please inquire for specifications

Required training with our staff for use of the MGCF Fluidigm Biomark machine.  Hands on training with your own samples. 

This service only includes the cost of training. Please separately purchase your desired chip format and reagents for training. 

Please contact the core for more information. 

Customer must provide Genechips, hybridization reagents, and washing reagents. 

Samples have already been labeled.

Please inform the core of the protocol used for ampllification and labeling. 

Customer must provide Genechips, amplification and labeling reagents, hybridization reagents, and washing reagents. 

If clean-up components are not included with the kits the customer must provide those as well.

Customer must provide Genechips and FlashTag Biotin HSR RNA Labeling Kit and hybriziation and washing reagents.

Price Per Two Plates

We have had to raise our prices due to a vendor raising their prices.  We are monitoring the situation and will lower our prices again as soon as we can. 

• Please send us at least 3 μg of high quality gDNA per sample. Samples should have a concentration of at least 30-50 ng/ul in nuclease-free water or TE buffer.
• Samples should be analyzed for quality and purity by gel electrophoresis using a 1% agarose gel; must be free of RNA contamination and show no signs of degradation. (Please send us a copy of gel image along with your samples).  If you cannot do this we can do it for you.
• Samples should have an A260/A280 ≥ 1.8 and an A260/A230 ≥ 1.6.
• If both minimum ratio values listed above are not met please perform additional precipitation before shipping your samples to us. High-quality genomic DNA is absolutely essential for successful outcomes. PICOGREEN Do NOT nanodrop!!!!!! Not sufficient!

1) Sample: The client will submit a minimum of 1.4ug of gDNA as the starting material.

2) Microarray: The microarray will be performed using Illumina’s Methylation EPIC array using related instrumentations and softwares.

3) Deliverables: Raw data in spreadsheet as the deliverable for this project.  The resulting data will be made available via FTP download.

4) TAT: Turnaround time is approximately 3-4 weeks after sample has passed initial QC.

5) Additional note: This project is for research use only.

Price is per run. 

Please book time under the Equipoment Reservations tab. 

Prices change frequently- please email for updated quote.

 Molecular Genetics Core does QC and send-out.

Price is per 12 samples. 12 sample minimum charge.

Self service extractions using the Qiagen QIACube.  User provides Qiagen kit. We provide reagent bottles, tips, tubes, and rotor adaptors necessary for the Qiacube.

 The pricing is per run. A run can accomodate 2-12 samples.

 Contact us via email for reserving the machine.

Customers must provide all reagents and slides, including gaskets, wash buffers, and Human Cot-1.

This platform enables to quickly and reliably identify aneuploidies, microdeletions, microduplications, as well as other types of chromosomal aberrations across the genome. ; Agilent’s CGH platform offers real comparative genomic data thanks to the 2-color approach, and unlimited flexibility in terms of format and content. Learn more about our catalog and custom microarrays.

Coding and non-coding human sequences represented

Probes are annotated against NCBI Build 38 (UCSC GRCh38, Dec. 2013)

Probe design and selection have been carefully optimized and validated for maximal sensitivity and specificity

Agilent provides validated protocols and reagents to process fresh frozen (SureTag Labeling Kit) and FFPE samples (ULS Labeling Kit.)

Arrays and reagents included. 

Customers must provide all reagents and slides, including gaskets, wash buffers, and Human Cot-1.

This platform enables to quickly and reliably identify aneuploidies, microdeletions, microduplications, as well as other types of chromosomal aberrations across the genome. ; Agilent’s CGH platform offers real comparative genomic data thanks to the 2-color approach, and unlimited flexibility in terms of format and content. Learn more about our catalog and custom microarrays.

Coding and non-coding human sequences represented

Probes are annotated against NCBI Build 38 (UCSC GRCh38, Dec. 2013)

Probe design and selection have been carefully optimized and validated for maximal sensitivity and specificity

Agilent provides validated protocols and reagents to process fresh frozen (SureTag Labeling Kit) and FFPE samples (ULS Labeling Kit.)

Arrays and reagents included. 

Self service extractions using the Qiagen QIACube.  User provides Qiagen kit. We provide reagent bottles, tips, tubes, and rotor adaptors necessary for the Qiacube.

 The pricing is per run. A run can accomodate 2-12 samples.

 Contact us via email for reserving the machine.

Assistance with data movement and transfer.  1 hour minimum.

Pricing listed is per 8 sample (column based). Please enter per column.

Investigator provides assay and restriction enzyme if necessary.

***Please clearly specify how materials need to be stored***

Required training with our staff for use of the MGCF Fluidigm Biomark machine.  Hands on training with your own samples. 

This service only includes the cost of training. Please separately purchase your desired chip format and reagents for training. 

Please contact the core for more information. 

Customer must provide Genechips, hybridization reagents, and washing reagents. 

Samples have already been labeled.

Please inform the core of the protocol used for ampllification and labeling. 

Customer must provide Genechips, amplification and labeling reagents, hybridization reagents, and washing reagents. 

If clean-up components are not included with the kits the customer must provide those as well.

Customer must provide Genechips and FlashTag Biotin HSR RNA Labeling Kit and hybriziation and washing reagents.

NanoString nCounter® MAX Profiler:  A simple, cost effective solution for multiplexing 10 – 800 targets in a single tube.

• Upgraded from the nCounter Sprint to the nCounter Max!
• Eliminate the need for cDNA synthesis, PCR or library prep by using this simple hybridization technology. 
• Assay DNA, RNA, miRNA or protein in 24 hours without the need for technical replicates. 
• Flexible sample input allows you to use purified RNA, cell lysate, blood, FFPE.
• Easy data analysis done at your desk with free software(nSolver software) for quick, publication quality figures.
• Pre-designed panels or custom options available through Nanostring.  For assistance, please contact Brendan Collins at <bcollins@nanostring.com>.
  

Price is per run. 

Please book time under the Equipoment Reservations tab. 

Analysis of Bionano Saphyr data.  Price is per sample.  Deliverable is a research report.

Please note that the MGCF is NOT a CLIA facility.

Please inquire for details and specifications.

please inquire for specifications

We have had to raise our prices due to a vendor raising their prices.  We are monitoring the situation and will lower our prices again as soon as we can. 

• Please send us at least 3 μg of high quality gDNA per sample. Samples should have a concentration of at least 30-50 ng/ul in nuclease-free water or TE buffer.
• Samples should be analyzed for quality and purity by gel electrophoresis using a 1% agarose gel; must be free of RNA contamination and show no signs of degradation. (Please send us a copy of gel image along with your samples).  If you cannot do this we can do it for you.
• Samples should have an A260/A280 ≥ 1.8 and an A260/A230 ≥ 1.6.
• If both minimum ratio values listed above are not met please perform additional precipitation before shipping your samples to us. High-quality genomic DNA is absolutely essential for successful outcomes. PICOGREEN Do NOT nanodrop!!!!!! Not sufficient!

1) Sample: The client will submit a minimum of 1.4ug of gDNA as the starting material.

2) Microarray: The microarray will be performed using Illumina’s Methylation EPIC array using related instrumentations and softwares.

3) Deliverables: Raw data in spreadsheet as the deliverable for this project.  The resulting data will be made available via FTP download.

4) TAT: Turnaround time is approximately 3-4 weeks after sample has passed initial QC.

5) Additional note: This project is for research use only.

Prices change frequently- please email for updated quote.

Price Per Two Plates

 Molecular Genetics Core does QC and send-out.

Price is per 12 samples. 12 sample minimum charge.

This instrument is self service. Please request under the Equipment Reservations tab. 

The CFX384 Touch Real-Time PCR Detection System is a powerful and precise real-time PCR instrument in a 384-well format, for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System utilizes accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions.

Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen, or use CFX Maestro Software to easily and intuitively design your experiment and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR.

Key Features and Benefits

With the CFX384 Touch Real-Time PCR Detection System you can:

• Obtain precise quantification and multiplex target discrimination – optical system uses long-lasting solid-state technology with 5 filtered LEDs and 5 filtered photodiodes
• Confirm system performance and data reliability – internal system qualification and system test software, automated data quality control, and various validation tool services are available.
• Obtain accurate results with volumes as low as 3 µl – minimize sample and reagent usage
• Use advanced data analysis tools — normalized gene expression using CFX Maestro Software
• CFX Maestro Software Works Seamlessly with PrimePCR™ Assays and Panels. The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Maestro Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.
• Begin analyzing data in a single step by importing target, reference, and control information from a PrimePCR run file directly into CFX Maestro Software
• Combine data from multiple plates into a Gene Study to rapidly screen large numbers of targets or samples
• Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation