Optical Imaging & Vital Microscopy Core (OiVM)




Welcome to the Optical Imaging & Vital Microscopy (OiVM) Core at Baylor College of Medicine. 

The mission of the OiVM Core is to provide state-of-the-art optical sectioning instrumentation as well as cutting edge image analysis tools for the research applications of a broad range of BCM investigators. This core is dedicated to vital and intravital imaging of cellular processes within cells, intact tissue explants, developing embryos and functioning organs within the live animal. Our users are focused on a variety of applications such as understanding cell migration, optimizing angiogenic therapies, how blood flow influences development and cancer, immune cell recruitment, stem cell-niche interactions and cancer metastasis.

 

OiVM Imaging Technologies

  • Confocal Microscopy
  • Lightsheet Microscopy
  • MultiPhoton Microscopy
  • High Speed Confocal Microscopy
  • µCT Scanning
  • OPT (Optical Projection Tomography)
  • OCT (Optical Coherence Tomography)
  • Fluorescence Stereomicroscopy
  • Live imaging of tissue/organ development in embryos and live animals
  • Live imaging of flowing fluorescent particles such as fluorescent beads, fluorescently labeled erythroblasts, etc.
  • Excitation and emission fingerprinting of fluorescent markers 

OiVM Image Analysis

  • 3D rendering of fluorescent structures in z-stack images
  • Automated nuclear counting
  • Detection and tracking of fluorescently labeled particles to measure their displacement, speed, etc.
  • Quantifying vascular morphology using classical measures and complexity measures such as fractal dimension
  • Enhanced image analysis using parallel computation on multicore processors 

   

Our Process

The OiVM Core operates primarily as an independent use core, where users are trained to operate the microscopes independently and are granted 24/7 access to book sessions whenever their schedule allows.

To get started using our core:

Step 1: Fill out our User Request Form with your contact information and a detailed description of your imaging needs.

Step 2: Consultation - Once we receive your information, a member of our core staff will contact you to set up a time to meet and discuss your project in greater detail.  This meeting will allow us to assess your needs and guide you to the best possible instrument for your experiments.

Step 3: Training - Once we've discussed your needs and have chosen an instrument that would best compliment your experiments, we will arrange an in-depth training session on the microscope of choice.  This comprehensive training will be designed to give you all the tools you need to become an independent core user.

Step 4:  Independent Use - Once your training is complete, you will be granted 24/7 access to the core facility and you can schedule time on our instruments as your schedule allows!

 

Our Team

 

Jason M. Kirk | Technical Director

Office: Cullen 113AB
Phone: 713-798-OIVM (6486)
Email: jason.kirk@bcm.edu

 

Chih-Wei (Logan) Hsu, Ph.D. | Co-Director

Office: Cullen 113AA
Email: loganh@bcm.edu

 

Where Are We?

Location

 Hours

Cullen Building, Rm 114A

Baylor College of Medicine

One Baylor Plaza

Houston, TX 77030

Phone:  (713) 798-OIVM (6486)

Contact Email:  oivm@bcm.edu 

8 a.m. - 6 p.m., Monday to Friday

Core Approved Users: 24/7

 

Visit Our Website

http://www.bcm.edu/oivm

Publication Acknowledgements 

Scientific publications containing data obtained using the services offered by the core should include the following statement in the acknowledgment section: This project was supported by the Optical Imaging & Vital Microscopy (OiVM) core at BCM.

Contacts

Name Role Phone Email Location
Jason M. Kirk
Core Director
 
713-798-OIVM (6486)
 
jason.kirk@bcm.edu
 
Cullen 113AB
 
Chih-Wei (Logan) Hsu, Ph.D.
Co-Director
 
713-798-OIVM (6486)
 
loganh@bcm.edu
 
Cullen 113AA