Mouse Gene Manipulation Core

This core no longer uses iLab. Please contact Tam directly at:

margaret.thompson@childrens.harvard.edu

617-919-2128

CLS 12122

Overview of Services

The Mouse Gene Manipulation Core offers mouse embryonic stem (ES) cell culture services and embryo microinjection and surgical implantation services required to make genetically altered mice, including gene targeted and transgenic mice.

Gene Targeting

In the mouse, specific mutations can be “targeted” to a desired location in the genome. Gene targeting exploits the pluripotency of mouse embryonic stem (ES) cells which, when injected into host mouse embryos, are capable of generating germ cells (sperm and eggs) that can pass their genetic content on to subsequent generations. ES cells are manipulated in culture to alter endogenous ES cell genes with specific mutations. These “targeted” ES cells are then injected into mouse embryos that are grown to fully mature mice. ES cells contribute to the development of the germ cells, and the mating of these mice result in the passage of the targeted mutation to the next generation of mice.

Transgenics

Transgenic mouse lines are those in which exogenous DNA (transgenes) are randomly integrated into the genome. Standard transgenic technology involves the microinjection of transgenic DNA sequences into the pronuclei of single-cell embryos. In a subset of the injected embryos, the injected DNA will stably insert into the mouse genome and be transmitted to subsequent generations upon breeding of the adult animals.

Core Services

1. Growth/transfection/selection/freezing of mouse ES cells to generate clones with desired mutations.

2. Blastocyst microinjection of ES cells and surgical implantation.

3. Pronuclear microinjection of DNA and surgical implantation.

4. Consultation for project design

5. Reagents

Leadership

Margaret Anne Thompson, PH.D. | Core Manager

Instructor in Neurology

Phone: 617-919-2128

Email: margaret.thompson@childrens.harvard.edu

Location and hours of operation

Hours	Location
[ Replace with hours of operation. ]	[ Replace with building and room number. ]

Links and Resources

http://core.iddrc.org/gene-manipulation/

Contacts

Name	Role	Phone	Email	Location
Margaret Thompson	Core Manager	617-919-2128	margaret.thompson@childrens.harvard.edu