The University of Chicago Cytometry and Antibody Technology Facility

Welcome to the Cytometry and Antibody Technology Facility 

How to Use iLab to Schedule Cytometers

 

Let us know about your publications

 

The CAT Facility is funded in part by the Cancer Center Support Grant and as such is mandated to keep track of publications where work was done within the facility. Please help us by filling out this form.

 

Acknowledgement of core facility contributions in publications is appreciated.

 

Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.

 

News from the CAT Facility

06/12/20 Updated COVID-19 related Operation. See the CAT Facility RRP for instructions on making reservations on iLab.

 

03/17/20 Important message regarding the COVID-19 situation and the CAT Facility: Operation in the facility and cell sorting protocol information

 

11/25/19: Help us streamline our operations by providing your FAS or PO number or your IRB code if you are using patient samples. See here on how that can be done in iLab.

11/25/19: If you need immediate assistance form the CAT Facility staff, send an email to cathelp@bsd.uchicago.edu.

 

 08/13/19: We have a new Website!! The CAT Facility website has been fully rebuilt and now includes more information then ever before!

  • Everything you need to know about the instruments, our services and pricing is a click away
  • The Instrument Status tool lets you know how our machines a doing and when repairs is scheduled
  • The Blog keeps you up to date with the latest news in the Facility
  • Check out the event page to learn about the next training classes, seminars and instrument demo.

 

New users

Welcome to the flow cytometry service laboratory. Please visit the New Users Guide on our website to learn of our policies.

 

The first step you will need to complete to use our services will be to create your account on the iLab scheduler - there is a Sign Up link at the top right of this page.

 

Once you are logged in the scheduler, you will be able to select the service you need.

 

The core offers flexibility in the way you want to work with the equipment. You can be trained on any instruments and run the samples on your own or you can get the CAT Facility experts to process the samples for you. In general, users will prefer to be trained on the benchtop analyzers and let the staff run the cell sorters.

 

Analyzers

To run your samples on a benchtop analyzers, go to Schedule Equipment, then select the Benchtop Analyzers, the Image Stream Mark II or the Multiplex schedule and reserve your time.  You will need to go through the training sessions before we allow you to use the instruments on your own.

To have the flow lab staff run your samples on an instrument on your behalf, go to Request Services and fill out the drop-off form.  No training is required.

 

Cell sorting

To use a cell sorter, go to Schedule Equipment and book time on the Cell Sorter: BD FACSAria or Cell sorter: BD FACS Fusion schedule. Fill out the form to let the operator know about your experiment and potential biohazards. Contact the staff for any inquiries about the amount of time neeeded to sort, proper sample preparation, etc.

 

Training

The flow lab offers training of each of our instruments with the exception of the Helios Mass Cytometer. Visit the Event page on the CAT FAcility website to get started.

 

Data transfer

All data coming from our instrument will transfer through the flow lab server. Contact the lab to get the credentials. Data left on the instrument computers will be deleted after 30 days. Data transferred to the server is not deleted. However, this is only offered as a support and the user remain the ultimate agent responsible for the safekeeping of the data.

 

 

Leadership

Anne Sperling, PhD. | Faculty Director

Email: asperlin@medicine.bsd.uchicago.edu

Phone: (773) 834-1211

 

David Leclerc, M.A. | Technical Director

Email: dleclerc@bsd.uchicago.edu

Phone: (773) 702-5582

 

Laura Johnston, PhD. | Associate Scientific Director

email: ljohnston@bsd.uchicago.edu

Phone: (773) 702-5582

 

Location and hours of operation

Hours Location

Assisted Services: 10 a.m. - 5 p.m.

Unassisted/Self Use: 24/7

BSLC

Room R022

Links and Resources

  1. http://ucflow.uchicago.edu/
  2. Campus Map

Contacts

Name Role Phone Email Location
Anne Sperling, PhD.
Faculty Director, FCF.
 
(773) 834-1211
 

 

 
David Leclerc MSc
Technical Director
 
(773) 702-5582
 
dleclerc@bsd.uchicago.edu
 

 
Laura Johnston, PhD.
Associate Scientific Director
 
(773) 702-5582
 
ljohnston@bsd.uchicago.edu
 

 
Michael Olson
Facility Manager
 
(773) 702-9212
 
molson@bsd.uchicago.edu
 

 
Mandel Davis
Senior Technology Specialist
 
(773) 702-9212
 
mdavis4@bsd.uchicago.edu
 

 
Robert Ladd
Technology Specialist
 
(773) 702-9212
 
rladd@bsd.uchicago.edu