The Analytical Genomics Core Facility at Lake Nona offers state-of-the-art genomic services such as Next Generation DNA Sequencing and DNA microarray technologies. We have already established these cutting-edge genomics technology platforms at Lake-Nona that complement related technologies in San Diego California. These combined resources provide access to our scientists, regardless of location, to some of the most advanced infrastructure for genomics research in the nation.
The Genomics Core is equipped with the following equipment:
Ranjan Perera, Ph.D | Scientific Director
Hours |
Location |
9:00 am - 5:00 pm |
Lake Nona Campus, A2916 6400 Sanger Road Orlando, FL 32827 |
Name | Role | Phone | Location | |
---|---|---|---|---|
Rajan Perera, Ph.D. |
Scientific Director
|
407.745.2084
|
rperera@sanfordburnham.org
|
Lake Nona Campus
|
Subramaniam Shyamala Govindarajan |
Research Associate III
|
407.745-6036
|
ssgovind@sanfordburnham.org
|
Lake Nona Campus
|
John Marchica |
Research Associate II
|
407.745.2132
|
jmarchica@sanfordburnham.org
|
Lake Nona Campus
|
Price List |
► Agilent Bioanalyzer 2100 (4) | |||
Name | Description | Price | |
---|---|---|---|
DNA 1000 Chip |
The DNA 1000 chip is ideal for separation, sizing and quantification of low concentrated dsDNA (PCR fragments, restriction digests or fragmented DNA) samples from 25 - 1000bp. The core charges for this service by chip. Each chip can accomodate up to 12 samples.
Sample Requirements: Concentration: 0.1 to 50 ng/ul Volume: 2 - 3 ul |
Inquire | |
High Sensitivity DNA Chip |
The High Sensitivity DNA chip is ideal for separation, sizing and quantification of low concentrated ds DNA (PCR fragments, restriction digests or fragmented DNA) samples from 50 - 7000bp. The core charges for this service by chip. Each chip can accomodate up to 11 samples.
Sample Requirements: Concentration: 50 to 500 pg/ul Volume: 2 - 3 ul
|
Inquire | |
RNA Nano chip |
The RNA Nano chip evaluates total RNA (Prokaryotic and Eukaryotic) and provide a RIN value on a scale of 1 to 10 (1=lowest & 10=Highest) as an indication of RNA quality. The core charges for this service by chip. Each chip can accomodate up to 12 samples.
Sample Requirements: Concentration: 50 to 500ng/ul Volume: 2 - 3 ul
|
Inquire | |
Small RNA Chip |
The Small RNA chips provide separation and analysis of nucleic acids below 150nt, with a final calculation of % miRNA. The core charges for this service by chip. Each chip can accomodate up to 11 samples.
Sample Requirements: Concentration: 50 to 2000 pg/ul Volume: 2 - 3 ul
|
Inquire | |
► Microarray (34) | |||
Name | Description | Price | |
Affymetrix Machine Usage |
Per Array Charge. Affymetrix machine usage to Hybridize, Wash and Scan Arrays. |
Inquire | |
Agilent One Color/Two Color Spike In Controls |
Per Reaction |
Inquire | |
Agilent Scanner Usage |
Charge per Slide
|
Inquire | |
Agilent Wash & Scan Protocol |
Charges for 1 to 3 Spotted Array Slides. Agilent machine usage to Wash & Scan Microarray Slides.
Services:
|
Inquire | |
Exon Expression Profiling - Human |
Full service - Total RNA to Data (Array Included). Genome-wide profiling of gene expression, including alternative transcript/exon analysis using Affymetrix Human Exon 1.0/2.0 ST GeneChip Arrays.
Please click the link to find more information about the array: Human Exon Array |
Inquire | |
Exon Expression Profiling - Mouse |
Full service - Total RNA to Data (Array Included). Genome-wide profiling of gene expression, including alternative transcript/exon analysis using Affymetrix Mouse Exon 1.0 ST GeneChip Arrays.
|
Inquire | |
Exon Expression Profiling - Rat |
Full service - Total RNA to Data (Array Included). Genome-wide profiling of gene expression, including alternative transcript/exon analysis using Affymetrix Rat Exon 1.0 ST GeneChip Arrays. Please click the link to find more information about the array: Rat Exon Array
|
Inquire | |
Gene Expression Profiling - Human |
Full service - Total RNA to Data (Array Included). Genome wide profiling of gene expression using Affymetrix Human Gene 1.0/2.0 ST GeneChip Arrays.
Please click the link to find more information about the array: Human Gene Array
|
Inquire | |
Gene Expression Profiling - Mouse |
Full service - Total RNA to Data (Array Included). Genome wide profiling of gene expression using Affymetrix Mouse Gene 1.0/2.0 ST GeneChip Arrays.
Please click the link to find more information about the array: Mouse Gene Array
|
Inquire | |
Gene Expression Profiling - Rat |
Full service - Total RNA to Data (Array Included). Genome wide profiling of gene expression using Affymetrix Rat Gene 1.0/2.0 ST GeneChip Arrays.
Please click the link to find more information about the array: Rat Gene Array
|
Inquire | |
Gene Expression Profiling Chemistry |
WT Assays (Nugen Protocol) |
Inquire | |
Gene Expression Profiling Chemistry (3` Assays) |
Full service - Total RNA to Data (Array Not Included). Complete Gene expression profiling chemistry for 3` Assays. Available only for over 30 organisms including Human, Mouse and Rat Species.
Services:
To view the Organism lists please click the link: Array Organism Lists |
Inquire | |
Gene/Exon Expression Profiling Chemistry (WT Assays) |
Full service - Total RNA to Data (Array Not Included). Complete Gene/Exon expression profiling chemistry for WT Assays. Available only for Human, Mouse and Rat Species.
Services:
|
Inquire | |
GeneChip C.elegans Genome Array |
Cost per Array
Please click the link to find more information about the array: C. elegans Array Information |
Inquire | |
GeneChip Drosophila Genome 2.0 Array |
Cost per Array
Please click the link to find more information about the array: Drosophila Array |
Inquire | |
GeneChip Human Exon 1.0 ST Array |
Cost per Array
Please click the link to find more information about the array: Human Exon Array |
Inquire | |
GeneChip Human Gene 1.0 ST Array |
Cost per Array
Please click the link to find more information about the array: Human Gene Array |
Inquire | |
GeneChip Human Genome U133 Plus 2.0 Array |
Cost per Array
Please click the link to find more information about the array: Human Genome U133 Array |
Inquire | |
GeneChip Mouse Exon 1.0 ST Array |
Cost per Array
Please click the link to find more information about the array: Mouse Exon Array |
Inquire | |
GeneChip Mouse Gene 1.0 ST Array |
Cost per Array
Please click the link to find more information about the array: Mouse Gene Array |
Inquire | |
GeneChip Mouse Genome 430 2.0 Array |
Cost per Array
Please click the link to find more information about the array: Mouse Genome 430 Array |
Inquire | |
GeneChip Rat Exon 1.0 ST Array |
Please click the link to find more information about the array: Rat Exon Array |
Inquire | |
GeneChip Rat Gene 1.0 ST Array |
Cost per Array
Please click the link to find more information about the array: Rat Gene Array |
Inquire | |
GeneChip Rat Genome 230 2.0 Array |
Cost per Array
Please click the link to find more information about the array: Rat Genome 230 Array
|
Inquire | |
GeneChip Yeast Genome 2.0 Array |
Cost per Array
Please click the link to find more information about the array: Yeast Genome 2.0 Array |
Inquire | |
Human Transcriptome Array Chemistry |
Per Reaction |
Inquire | |
LINC RNA Array |
8 x 60 Format |
Inquire | |
lncRNA Array - Human |
2x105K Format - Each array contains 2 subarray spots. Can process 2 samples in each slide. ABI's NCode high-density oligo arrays are use to profile long non-coding RNA's.
Please click the link to find more information about the array: lncRNA Human Array |
Inquire | |
lncRNA Array - Mouse |
2x105K Format - Each array contains 2 subarray spots. Can process 2 samples in each slide. ABI's NCode high-density oligo arrays are utilized to profile long non-coding RNA's.
Please click the link to find more information about the array: lncRNA Mouse Array |
Inquire | |
MicroRNA Profiling - Human |
Full service - Total RNA to Data(ArrayIncluded). Comprehensive miRNA profiling based on ABI's miRNA low density arrays provides close to full coverage of sanger miRbase. Available only for Human and Rodent species.
Our Services Include:
Please click the link to find more information about the array: Taqman Human miRNA V3.0 Array |
Inquire | |
MicroRNA Profiling - Rodent |
Full service - Total RNA to Data(Array Included). Comprehensive miRNA profiling based on ABI's miRNA low density arrays provides close to full coverage of sanger miRbase.
Our Services Include:
Please click the link to find more information about the array: Taqman Rodent miRNA V3.0 Array |
Inquire | |
Miscellaneous-Microarray | Inquire | ||
Target Labeling for lncRNA Profiling |
Target labeling will be performed using Agilent's Low Input Quick Amp labeling kit. Core is capable of doing both single color and dual color analysis using Cy3/Cy5 dye. (Total RNA to Labeled cRNA Hybridization)
Services:
Please click the link to find more information about the array: lncRNA labeling Information |
Inquire | |
Target Preparation (cDNA Syn) for miRNA Profiling |
cDNA Synthesis using ABI's Megaplex RT protocol for miRNA TLDA Profiling. |
Inquire | |
► QPCR (3) | |||
Name | Description | Price | |
7900HT Instrument Usage |
Cost Per Plate Run |
Inquire | |
PCR Reaction |
Per Sample |
Inquire | |
Reverse Transcription Reaction |
PER SAMPLE |
Inquire |