Cardiovascular Physiology Core

Overview of Services

The Cardiovascular Physiology Core is designed to provide reliable preclinical research expertise to our clients - academic, non-profit and corporate-based, who are interested in using proven disease models from zebrafish to rodents to assess therapeutics or establish novel models using small animals and fish. We have the capacity to facilitate research and training, from in vivo to ex vivo to in vitro models, using primary cells and cell lines and can engage in molecular discovery and exploratory research including biomarkers, using animal models and tissue/cells. Although we are primarily focused on cardiovascular disease area for surgery models, we have recently expanded to whole body imaging via a cutting edge preclinical ultrasound technology platform that is fast, reliable and affordable for most researchers. We offer extensive post-acquisition data analysis whereby researchers can quantify the obtained data in a robust and facile way including strain analysis and we can deliver contrast agents to all organs and perform post contrast quantitative analysis (eg. tumor models or hypoxia models). We can meet a variety of  translational needs of our research community and have engaged nationally and internationally, with an array of successful collaborations that have led to a large body of publications, grants, and even converted studies into clinical trial testing (small molecule and gene-therapy).  We offer both full-service and limited service options and are always open to new collaborations.

 Our services include but are not limited to: 

  •  High-Frequency Ultrasound Imaging of multiple organs (Vevo 3100)
  • Training + Assisted Service
  • Unassisted Service
  • Systemic in vivo Imaging and post-imaging Data Analysis (assisted, training or unassisted)
  • In vivo modeling of cardiac and other disease ( eg. lung injury, vasculopathy, cardiac & muscle diseases, renal pathologies, endocrine, cancer, neonatology, abdominal and inflammatory conditions, genetic disorders, cell and gene therapy, tissue remodelling)
  • Phenotypic screening using rodent and fish models 
  • Cell, gene and small molecule theraputics
  • Surgery (includes, but not limited to, the following procedures)(mouse and rat)
    • Myocardial infarction model 
    • Ischemic reperfusion model
    • Banding model (TAC, PAC, AAC)
    • Pulmonary Hypertension model (rats and mice)
    • Injections which could be intramyocardial or parenteral (rats, mice and fish)
  • Blood Pressure Measurement (invasive, cath-based and noninvasive)
  • Langendorff (ex vivo)
  • PV Loop relationships assessment and associated data analysis (ADI Instruments platform)
  • Isolated Heart function assessment capability (includes injections, tissue harvest, sample collection)
  • Cardiomyocyte (CM) and fibroblast (CF) Isolation (rats and mice, adult and neonatal)
  • Animal procurement, housing, transfer, import/export
  • Post-surgery monitoring (required for in vivo longitudinal survival studies)
  • High-throughput cardiac phenotyping
  • Delivery device implantation and safety assessment

We can work off-site for all preclinical needs 

 

 

Location 

 
We are located in the NRB building, 4th floor in Room 435F and 431A
 
BWH, NRB 0435F
77 Avenue Louis Pasteur
Boston, MA 02115

Contact: Phone: 617 525 4862

 BWH Cardiovascular Physiology Core Website

 

 

Contacts

Name Role Phone Email Location
Alex Loscalzo
Core Contact
 
(617) 525-4862
 
aloscalzo@partners.org
 
NRB431
 
Souen Ngoy
Surgeon
 
(617) 525 4864
 
sngoy@rics.bwh.harvard.edu
 
NRB431