ALERT

In Observance of U.S. holiday, Presidents Day, Agilent Cross Lab iLab Operations Software will be closed during U.S. hours and have support during EU and APAC hours on Monday February 20th, 2017. We will resume U.S. support hours on Tuesday February 21st, 2017, however, due to the Software User Group Conference, there may be a slight delay on general support requests. For urgent matters, please add "Urgent" to the ticket subject and we will prioritize those requests first.

Thank you,

Cell Imaging Core (La Jolla)

Getting Started

Internal SBPMDI

  • If you have an iLab account, please login at the top right or use this link https://ilab.sbpdiscovery.org/
  • If you do not have an iLab account, please click HERE to request an account. All fields are required.

 

External SBPMDI

  • You must have a Customer ID and iLab account to request services from this facility.
  • To obtain a Customer ID please email Eveline Hernandez at ehernandez@sanfordburnham.org
  • To obtain an iLab account, please click HERE.


Forgot your password? Please click HERE.

 

Overview of Services

 

The core houses a wide variety of microscopes, and provides training, assistance and use of the instruments, as well as full-service imaging.  The Cell Imaging Facility microscopes include six advanced fluorescence microscopes and three confocal systems (including a  Zeiss  LSM-710 NLO multiphoton microscope, Olympus FluoView-1000 and Yokagawa Spinning Disk confocal).  Sophisticated systems for FRET, Calcium imaging, and TIRF are also available.  The microscopes are integrated with advanced image capture and analysis software, and a number of instruments are equipped with environmental chambers for extended live cell analysis.

 

Services

  • Wide field microscopy:
    • bright field and dark field microscopy
    • phase and Nomarski differential interference contrast
    • polarization and epi-fluorescent microscopy
  • Single and multiphoton confocal microscopy
  • Live cell imaging
  • Image analysis:
    • morphometry
    • 3D and 4D rendering
    • deconvolution
  • Training and consultation

 

 

Equipment and Resources

  • Upright BX50 Olympus wide field fluorescence microscope with cooled, color CCD camera and an automated XYZ stage
  • Two inverted TE300 Nikon wide field fluorescence microscopes with cooled, color CCD cameras (the second one with an automated XYZ stage).
  • Inverted IX81 Olympus wide field fluorescence microscope for FRET and Ca2+ imaging, equipped with cooled monochrome CCD camera Cascade 512B, XYZ controlled stage and CO2/temperature controlled environmental chamber for long term time lapse imaging
  • Inverted IX81 Olympus wide field fluorescence microscope for TIRF time lapse imaging, equipped with cooled CCD monochrome CCD camera QuantEM and Piezo-Z controlled  objective stage; Kr-Ar laser emitting at 488, 568 and 647nm lines
  • Inverted IX81 Olympus wide field fully automated fluorescence microscope for basic and advanced methods of microscopy.  Microscope equipped with high resolution cooled monochrome CCD camera ORCA-ER.
  • Yokogawa Spinning Disk Laser Confocal Microscope with XY and Piezo-Z controlled objective stage (ASI  2000-Piezo); Kr-Ar laser emitting at 488, 568 and 647 nm lines, cooled, monochrome CCD camera QuantEM; “20/20” environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging
  • Zeiss LSM-710 NLO  Multiphoton Laser Point Scanning Confocal Microscope equipped with single photon Ar-ion laser emitting at 457, 479, 488 and 514 nm lines; DPSS solid state laser emitting 561 nm line; Red Diode laser, emitting at 633 nm line, and multi-photon Mai-Tai-HP_ DeepSee Laser; “20/20” environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging.
  • Fluoview 1000 Olympus Laser Point Scanning Confocal Microscope, single photon lasers (Diode laser emitting 405nm line; Argon-ion laser emitting at 457, 488 and 514 nm; solid state diod lasers emitting 561 nm and Red Diode laser, emitting at 633 nm); SIM Scanner with 405nm laser diode; Spectral Detection system; FRAP; FRET and photoactivation applications; Tokai Hit environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging
  • Software packages for image processing:
    • ImagePro Plus
    • Volocity 3D-4D rendering
    • Metamorph//MetaFluor
    • Spot RT acquisition and processing

 

Leadership

Leslie Boyd | Facility Manager

Location and hours of operation

Hours Location

Open 7 days a week,

24 hours per day.

Sanford-Burnham Medical Research Institute

La Jolla Campus, Bldg 5 – Rooms 5101, 5105, 5114, 5170

10901 North Torrey Pines Road
La Jolla, CA 92037