Cell Imaging Core (La Jolla)

Getting Started

Internal SBMRI

  • If you have an iLab account, please login at the top right or use this link https://ilab.sanfordburnham.org/
  • If you do not have an iLab account, please click HERE to request an account. All fields are required.

 

External SBMRI

  • You must have a Customer ID and iLab account to request services from this facility.
  • To obtain a Customer ID please email Eveline Hernandez at ehernandez@sanfordburnham.org
  • To obtain an iLab account, please click HERE.


Forgot your password? Please click HERE.

 

Overview of Services

The core houses a wide variety of microscopes, and provides training, assistance and use of the instruments, as well as full-service imaging.  Facility microscopes include seven advanced fluorescence microscopes, three confocal systems (including a  Zeiss  LSM-710 NLO multiphoton microscope, Olympus FluoView-1000 and Yokagawa Spinning Disk confocal) and a transmission electron microscope (Hitachi H-600A).  Sophisticated systems for FRET, Calcium imaging, and TIRF are also available.  The microscopes are integrated with advanced image capture and analysis software, and a number of instruments are equipped with environmental chambers for extended live cell analysis.

 

Services

  • Wide field microscopy:
    • bright field and dark field microscopy
    • phase and Nomarski differential interference contrast
    • polarization and epi-fluorescent microscopy
  • Single and multiphoton confocal microscopy
  • Live cell imaging
  • Electron microscopy
  • Image analysis:
    • morphometry
    • 3D and 4D rendering
    • deconvolution
  • Training and consultation

 

 

Equipment and Resources

  • Upright BX50 Olympus wide field fluorescence microscope with cooled, color CCD camera and an automated XYZ stage
  • Two inverted TE300 Nikon wide field fluorescence microscopes with cooled, color CCD cameras (one with a microinjection attachment and the second one  with an automated XYZ stage).
  • Inverted IX81 Olympus wide field fluorescence microscope for FRET and Ca2+ imaging, equipped with cooled monochrome CCD camera Cascade 512B, XYZ controlled stage and CO2/temperature controlled environmental chamber for long term time lapse imaging
  • Inverted IX81 Olympus wide field fluorescence microscope for TIRF time lapse imaging, equipped with cooled CCD monochrome CCD camera QuantEM and Piezo-Z controlled  objective stage; Kr-Ar laser emitting at 488, 568 and 647nm lines
  • Inverted IX81 Olympus wide field fully automated fluorescence microscope for basic and advanced methods of microscopy.  Microscope equipped with high resolution cooled monochrome CCD camera ORCA-ER.
  • Yokogawa Spinning Disk Laser Confocal Microscope with XY and Piezo-Z controlled objective stage (ASI  2000-Piezo); Kr-Ar laser emitting at 488, 568 and 647 nm lines, cooled, monochrome CCD camera QuantEM; “20/20” environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging
  • Zeiss LSM-710 NLO  Multiphoton Laser Point Scanning Confocal Microscope equipped with single photon Ar-ion laser emitting at 457, 479, 488 and 514 nm lines; DPSS solid state laser emitting 561 nm line; Red Diode laser, emitting at 633 nm line, and multi-photon Mai-Tai-HP_ DeepSee Laser; “20/20” environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging.
  • Fluoview 1000 Olympus Laser Point Scanning Confocal Microscope, single photon lasers (Diode laser emitting 405nm line; Argon-ion laser emitting at 457, 488 and 514 nm; solid state diod lasers emitting 561 nm and Red Diode laser, emitting at 633 nm); SIM Scanner with 405nm laser diode; Spectral Detection system; FRAP; FRET and photoactivation applications; Tokai Hit environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging
  • Electron microscope Hitachi H600A
  • Software packages for image processing:
    • ImagePro Plus
    • Volocity 3D-4D rendering
    • Metamorph//MetaFluor
    • Spot RT acquisition and processing

 

Leadership

Leslie Boyd | Facility Manager

Location and hours of operation

Hours Location

Open 7 days a week,

24 hours per day.

Sanford-Burnham Medical Research Institute

La Jolla Campus, Bldg 5 – Rooms 5101, 5105, 5114, 5170

10901 North Torrey Pines Road
La Jolla, CA 92037