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Overview of Services
The core houses a wide variety of microscopes, and provides training, assistance and use of the instruments, as well as full-service imaging. The Cell Imaging Facility microscopes include six advanced fluorescence microscopes and three confocal systems (including a Zeiss LSM-710 NLO multiphoton microscope, Olympus FluoView-1000 and Yokagawa Spinning Disk confocal). Sophisticated systems for FRET, Calcium imaging, and TIRF are also available. The microscopes are integrated with advanced image capture and analysis software, and a number of instruments are equipped with environmental chambers for extended live cell analysis.
- Wide field microscopy:
- bright field and dark field microscopy
- phase and Nomarski differential interference contrast
- polarization and epi-fluorescent microscopy
- Single and multiphoton confocal microscopy
- Live cell imaging
- Image analysis:
- 3D and 4D rendering
- Training and consultation
Equipment and Resources
- Upright BX50 Olympus wide field fluorescence microscope with cooled, color CCD camera and an automated XYZ stage
- Two inverted TE300 Nikon wide field fluorescence microscopes with cooled, color CCD cameras (the second one with an automated XYZ stage).
- Inverted IX81 Olympus wide field fluorescence microscope for FRET and Ca2+ imaging, equipped with cooled monochrome CCD camera Cascade 512B, XYZ controlled stage and CO2/temperature controlled environmental chamber for long term time lapse imaging
- Inverted IX81 Olympus wide field fluorescence microscope for TIRF time lapse imaging, equipped with cooled CCD monochrome CCD camera QuantEM and Piezo-Z controlled objective stage; Kr-Ar laser emitting at 488, 568 and 647nm lines
- Inverted IX81 Olympus wide field fully automated fluorescence microscope for basic and advanced methods of microscopy. Microscope equipped with high resolution cooled monochrome CCD camera ORCA-ER.
- Yokogawa Spinning Disk Laser Confocal Microscope with XY and Piezo-Z controlled objective stage (ASI 2000-Piezo); Kr-Ar laser emitting at 488, 568 and 647 nm lines, cooled, monochrome CCD camera QuantEM; “20/20” environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging
- Zeiss LSM-710 NLO Multiphoton Laser Point Scanning Confocal Microscope equipped with single photon Ar-ion laser emitting at 457, 479, 488 and 514 nm lines; DPSS solid state laser emitting 561 nm line; Red Diode laser, emitting at 633 nm line, and multi-photon Mai-Tai-HP_ DeepSee Laser; “20/20” environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging.
- Fluoview 1000 Olympus Laser Point Scanning Confocal Microscope, single photon lasers (Diode laser emitting 405nm line; Argon-ion laser emitting at 457, 488 and 514 nm; solid state diod lasers emitting 561 nm and Red Diode laser, emitting at 633 nm); SIM Scanner with 405nm laser diode; Spectral Detection system; FRAP; FRET and photoactivation applications; Tokai Hit environmental chamber provides CO2/temperature controlled conditions for long term time lapse imaging
- Software packages for image processing:
- ImagePro Plus
- Volocity 3D-4D rendering
- Spot RT acquisition and processing
Leslie Boyd | Facility Manager
Location and hours of operation
Open 7 days a week,
24 hours per day.
Sanford-Burnham Medical Research Institute
La Jolla Campus, Bldg 5 – Rooms 5101, 5105, 5114, 5170
10901 North Torrey Pines Road
La Jolla, CA 92037