Stem Cell Core

Overview of Services

Mission Statement:

Our mission is to provide an excellent physical and intellectual environment for researchers to carry out cutting-edge stem cell research for biological and biomedical discovery.

About the Core:

Gladstone Stem Cell Core (GSCC) is a shared research facility that provides service, training, reagents and equipment for research using human and mouse embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. We provide everything needed to culture, manipulate and image stem cells, including biosafety cabinets, plasticware, media, reagents, microscopes. We offer comprehensive training for researchers interested in incorporating stem cell experiments into their projects and strive to help you implement emerging technologies for stem cell studies. In addition, we provide expert services, and welcome custom projects and collaborations, for research involving human induced pluripotent stem cells (iPSCs).

GSCC provides all of the resources required for research using mouse and human ESCs and iPSCs:

A furnished facility with 16 tissue culture hoods, >20 incubators, media, reagents, tissue culture plasticware, and iPSC-related lines
Two fluorescence microscopy systems with GFP, RFP and CFP filters and long-term, time-lapsed movie capabilities
Automated cell counting equipment
Nucleoporation equipment (Lonza Nucleofector X and Y units)
Two aseptic AMG EVOS colony picking microscopes
IncuCyte S3 for live cell imaging and analyses
Axion Maestro Microelectrode Array system
Human iPSC-related standard and custom scientific services
An intellectual environment rich in collaboration and idea exchange among its researchers and Core staff

Location and Hours of Operation

The Gladstone Institutes

1650 Owens St, San Francisco, CA 94158
Stem cell core office - Room 428
Cell culture facility - Rooms 426, 427, 463 and 461

Director's office - Room 421

Facility is open 24/7 (Access Badge required at weekends and non-business hours)

* Material Order Procurement / iLab reagent orders: supplied 10am - Noon Mondays, Wednesdays, and Fridays in Room 428

Getting Started

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Visit the Customer Help Manual for further guidance using iLab's Core Facility Services.

Leadership

Ritu Kumar - Core Director

Email: ritu.kumar@gladstone.ucsf.edu

Phone: 415-734-4924

Fax: 415-355-0960

Core Staff

Wendy Runyon - Research Scientist and Lab Manager

Email: wendy.runyon@gladstone.ucsf.edu

Phone: 415-734-2866

Fax: 415-355-0960

Links and Resources

Core homepage: http://www.gladstone.ucsf.edu/gladstone/site/escl/

Contacts

Name	Role	Phone	Email	Location
Ritu Kumar	Core Director	415-734-4924	ritu.kumar@gladstone.ucsf.edu	Room 421
Wendy Runyon	Core Staff	415-734-2866	wendy.runyon@gladstone.ucsf.edu	Room 428

Fixed Cost Services & Product Requests

Name	Description	Price
1. Human iPSC Culture Training - per person	This is a hands-on training by expert core staff using a control iPSC line WTC (or an equivalent line). Training will cover how to thaw, passage, and freeze human iPSCs, along with sterile tissue culture techniques. Price includes reagents, media, and hands-on instruction. (Approximately 2 weeks.)	Inquire
1a. Mycoplasma screening - offered every Friday	Drop off samples to Room 428 before 1pm on Fridays. Please contact stemcell-core@gladstone.ucsf.edu for any questions about sample requirements.	Internal Gladstone $26.87 each UCSF $38.69 each Corporate $67.17 each External (non-profit) $51.00 each
1b. Mycoplasma Quarterly screening	Drop off samples to Room 428 before 1pm on the designated quarterly mycoplasma screening day. Please contact stemcell-core@gladstone.ucsf.edu for any questions about sample requirements.	Internal Gladstone $20.00 each UCSF $28.80 each Corporate $60.00 each
2. iPSC Colony Picking Training	This is a hands-on training by expert core staff using a control iPSC line WTC (or an equivalent line). Training will cover how to manually pick iPSC colonies for gene editing or reprogramming. Price includes reagents, media, and hands-on instruction. (Duration: 2 days)	Inquire
3. Cardiac Differentiation Training	This is a hands-on training by expert core staff using a control iPSC line WTC (or an equivalent line known to differentiate). Training will cover cardiac differentiation using the Wnt method up to Day 15. Price includes reagents, media, and hands-on instruction. (Approximately 3 weeks.)	Inquire
4. iPSC Reprogramming Training - Episomal Method from PBMCs	This is a hands-on training by expert core staff using control PBMCs (that have previously been successfully reprogrammed) and an experimental PBMC sample (requestor's choice). Training will cover: - Transfection of PBMCs with plasmids containing Yamanaka factors - Plating and maintenance of transfected cells - Identification of newly formed iPSC colonies - Picking iPS colonies onto matrigel coated wells - Training to expand and freeze iPSCs for viable recovery Price includes reagents, media, and hands-on instruction. (Approximately 1-2 months.)	Inquire
A. STEMdiff Cardiomyocyte Differentiation Service Kit - for service request only	Reagents and media for cardiomyocyte differentiation of 1 iPSC line. Includes 2-3 iPSC passaging, and cardiomyocyte differentiation to Day 15. Price includes all media, reagents and STEMdiff kit Cat. #05010, #05020. Enough for 4X 12-well plate differentiations.	Internal Gladstone $1,700.00 each UCSF $2,380.00 each External (non-profit) $3,213.00 each Corporate $3,400.00 each
B. Invisible: STEMdiff Cardiomyocyte Differentiation Service - OPTIMAL Day 15 iPSC-cardiomyocytes	Labor cost for Cardiomyocyte Differentiation of 1 iPSC line using the STEMdiff Cardiomyocyte Differentiation and Maintenance Kits. Up to 4X 12-well plates. Optimal: >70% beating cells per well in 75% of wells (as measured by Cellogy) at optimal seeding density (iPSC line-specific).	Inquire
Basic Cell Line Identity Testing - 24 Codis STR Loci and Amelogenin - Live Culture	Service available Tuesdays (holidays excluded) before 2:30 p.m.	Inquire
C. Invisible STEMdiff Cardiomyocyte Differentiation Service - SUB-OPTIMAL Day 15 iPSC-cardiomyocytes	Labor cost for Cardiomyocyte Differentiation of 1 iPSC line using the STEMdiff Cardiomyocyte Differentiation and Maintenance Kits. Up to 4X 12-well plates. Sub-optimal: <70% beating cells per well in 75% of wells (as measured by Cellogy) at optimal seeding density (iPSC line-specific).	Inquire
CHDI Project - Bundle 8: Cortical differentiation	Invisible	Inquire
Cytogenetics service - G band Karyotyping - per cell line	Service available Tuesdays (holidays excluded) before 2:30 p.m. Submission Guidelines: Provide a 60-70% confluent T25 with plug-sealed cap filled entirely with media (no bubble) supplemented with 10%-15% FBS to increase cell survival during shipment. Also provide an additional 15 mL of media. Wrap the caps of both with parafilm. Fill out the CLG request sheet. Provide Core staff with request sheet and cells before 2:30 p.m.	Internal Gladstone $460.00 each
D. Wnt Method: Cardiomyocyte Differentiation Kit	Reagents and media for cardiac differentiation of 1 iPSC line by the Wnt method. Includes 2-3 iPSC passaging, and cardiac differentiation to Day 15. Up to 8X 12-well plates.	Internal Gladstone $1,811.25 each UCSF $2,716.88 each Corporate $3,441.37 each External (non-profit) $4,528.12 each
E. Invisible Wnt Method: Cardiomyocyte Differentiation Service - Optimal Day 15 iPSC-cardiomyocytes	Service for efficient cardiomyocyte differentiations. Optimal: >60% beating cells per well in 75% of wells (as measured by Cellogy) at optimal seeding density (iPSC line-specific). Up to 8X 12-well plates. Includes all differentiation reagents.	Inquire
F. Invisible:Wnt Method: Cardiomyocyte Differentiation Service - Sub-optimal Day 15 iPSC-cardiomyocytes	Costs for inefficient cardiomyocyte differentiations. Suboptimal: >60% beating cells per well in 75% of wells (as measured by Cellogy) at optimal seeding density (iPSC line-specific). Up to 8X 12-well plates.	Inquire
G. Invisible:Wnt Method: Cardiomyocyte Density Optimization Service	Density optimization for cardiomyocyte differentiation of new cell lines. Up to 2x 12-well plates will be set up with a range of 3-4 densities to determine optimal seeding density for new cell lines.	Inquire
Genome engineering iPSC line, Service A: Custom CRISPR gene knockout by NHEJ - FULL SERVICE: includes labor, reagents, equipment use	Clonal homozygous gene KO iPSC line with >80% ICE KO score (2 rounds of manual single cell clone picking) Deliverables: 2 subclones of 2 independent clonal iPSC lines with >80% ICE KO score (pure) 3 sgRNA used for gene editing 1 working primer pair for genotyping 1 working Sanger sequencing primer hiPSC line - WTC (optional) Progress report If no clones are isolated, another attempt will be made at no extra cost. For more information, please contact stemcell-core@gladstone.ucsf.edu	Internal Gladstone $7,000.00 each UCSF $9,563.00 each Corporate $17,077.50 each External (non-profit) $12,978.00 each
Growth Inactivated SNL - 1x10^6 cells/vial		Inquire
Growth Inactivated SNL - 3x10^6 cells/vial		Inquire
Human iPSC line: CRISPRi Gen2C, frozen cryovial	Tetracycline-inducible CRISPR interference system in WTC11 human iPSC line. Contains the Tet-on-3G system driving expression of dCas9-KRAB (in one allele of the AAVS1 locus) in the human WTC iPSC line. With doxycycline addition, dCas9-KRAB is expressed. The other AAVS1 allele is wildtype. This cell line has no fluorophores. It is useful for repressing specific genes in human iPSCs and in early cell lineages. 1e6 cells per vial. Passage 33.14. Normal karyotype at passage 33.14. Reference:CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs. Mandegar, MA, Huebsch, N, Frolov, EB, Shin, E, Truong, A, Olvera, MP, Chan, AH, Miyaoka, Y, Holmes, K, Spencer, CI, Judge, LM, Gordon, DE, Eskildsen, TV5, Villalta, JE, Horlbeck, MA, Gilbert, LA, Krogan, NJ, Sheikh, SP, Weissman, JS, Qi, LS, So, PL, Conklin BR. Cell Stem Cell. 2016 Apr 7;18(4):541-53. PMID: 26971820 NOTE: For external requests, a material transfer agreement with Gladstone is required. Please contact stemcell-core@gladstone.ucsf.edu	Inquire
Hypoxic Incubator - Weekly use	Weekly fee for use of the hypoxic incubator in room 461. Charge covers the cost of Nitrogen and incubator upkeep. Contact SCC staff at least one week in advance before needing to use the hypoxic incubator!	Inquire
iPSC line expansion and banking	Includes price of media and plastics, labor. You will receive 10 cryovials of 1 million cells/vial. Please provide karyotyped cells.	Internal Gladstone $1,000.00 each UCSF $1,440.00 each Corporate $2,500.00 each External (non-profit) $1,900.00 each
iPSC line expansion and banking - ADD ON Cleaning iPSC lines by manual picking of colonies and expansion	Add on to standard iPSC expansion and banking service for cell lines with significant spontaneous differentiation. Colonies with good iPCS morphology will be manually picked and expanded.You will receive 10 cryovials of 1 million cells/vial. Please provide karyotyped cells.	Inquire
PBMC isolation from whole blood /tube- RUSH Processing	Service includes isolation of PBMCs from fresh whole blood provided in CPT™ Vacutainer® (BD, Cat # 362761). Price per Vacutainer®. PBMCs will be frozen at 5e6 cells/vial unless otherwise specified. Contact the Stem Cell Core (stemcell-core@gladstone.ucsf.edu) prior to obtaining blood samples to schedule this service. Select this service for samples dropped off without at least one week's advance notice.	Inquire
PBMC isolation from whole blood/sample	Service includes isolation of PBMCs from fresh whole blood provided in CPT™ Vacutainer® (BD, Cat # 362761). Price per Vacutainer®. PBMCs will be frozen at 5e6 cells/vial unless otherwise specified. Contact the Stem Cell Core (stemcell-core@gladstone.ucsf.edu) at least one week prior to obtaining blood samples to schedule this service.	Internal Gladstone $350.00 each UCSF $504.00 each Corporate $875.00 each External (non-profit) $665.00 each
Project Consultation - Gene editing	Expert advice and consultation for iPSC and gene editing projects. The first consulation (~1h) is free-of-charge. Contact: stemcell-core@gladstone.ucsf.edu	Inquire
Z. (INVISIBLE) Human iPSC line: CRISPRi Gen1C, frozen cryovial	Invisible Tetracycline-inducible CRISPR interference system in WTC11 human iPSC line. Contains the Tet-on-3G system driving expression of dCas9-KRAB and mCherry (from one allele of the AAVS1 locus) in the human WTC iPSC line. With doxycycline addition, dCas9-KRAB and mCherry are expressed. In the other AAVS1 locus, there is a calcium biosensor, GCaMP6f, which is constitutively expressed. From Dr. Bruce Conklin's cell line collection. 1e6 cells per vial. Passage 47. Normal karyotype at passage 48. Reference: CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs. Mandegar, MA, Huebsch, N, Frolov, EB, Shin, E, Truong, A, Olvera, MP, Chan, AH, Miyaoka, Y, Holmes, K, Spencer, CI, Judge, LM, Gordon, DE, Eskildsen, TV5, Villalta, JE, Horlbeck, MA, Gilbert, LA, Krogan, NJ, Sheikh, SP, Weissman, JS, Qi, LS, So, PL, Conklin BR. Cell Stem Cell. 2016 Apr 7;18(4):541-53. PMID: 26971820 NOTE: For external requests, a material transfer agreement with Gladstone is required. Please contact stemcell-core@gladstone.ucsf.edu	Inquire
Z. Custom IPSC reprogramming from fibroblasts (all 3 phases) for Bridgebio		Inquire
Z. INVISIBLE - Human iPSC line: CRISPRi Gen1B - frozen cryovial	Tetracycline-inducible CRISPR interference system in WTB human iPSC line. Contains the Tet-on-3G system driving expression of dCas9-KRAB. From Dr. Bruce Conklin's cell line collection. Reference: CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs. Mandegar, MA, Huebsch, N, Frolov, EB, Shin, E, Truong, A, Olvera, MP, Chan, AH, Miyaoka, Y, Holmes, K, Spencer, CI, Judge, LM, Gordon, DE, Eskildsen, TV5, Villalta, JE, Horlbeck, MA, Gilbert, LA, Krogan, NJ, Sheikh, SP, Weissman, JS, Qi, LS, So, PL, Conklin BR. Cell Stem Cell. 2016 Apr 7;18(4):541-53. PMID: 26971820. NOTE: For external requests, a material transfer agreement with Gladstone is required. Please contact stemcell-core@gladstone.ucsf.edu	Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► CHDI Project ( Finkbeiner Lab ) (1)
CHDI Project - Bundle 8: Cortical differentiation	Invisible	Inquire
▼ ► Cardiac Differentiation Services (7)
Name	Description	Price
A. STEMdiff Cardiomyocyte Differentiation Service Kit - for service request only	Reagents and media for cardiomyocyte differentiation of 1 iPSC line. Includes 2-3 iPSC passaging, and cardiomyocyte differentiation to Day 15. Price includes all media, reagents and STEMdiff kit Cat. #05010, #05020. Enough for 4X 12-well plate differentiations.	Internal Gladstone $1,700.00 each UCSF $2,380.00 each External (non-profit) $3,213.00 each Corporate $3,400.00 each
B. Invisible: STEMdiff Cardiomyocyte Differentiation Service - OPTIMAL Day 15 iPSC-cardiomyocytes	Labor cost for Cardiomyocyte Differentiation of 1 iPSC line using the STEMdiff Cardiomyocyte Differentiation and Maintenance Kits. Up to 4X 12-well plates. Optimal: >70% beating cells per well in 75% of wells (as measured by Cellogy) at optimal seeding density (iPSC line-specific).	Inquire
C. Invisible STEMdiff Cardiomyocyte Differentiation Service - SUB-OPTIMAL Day 15 iPSC-cardiomyocytes	Labor cost for Cardiomyocyte Differentiation of 1 iPSC line using the STEMdiff Cardiomyocyte Differentiation and Maintenance Kits. Up to 4X 12-well plates. Sub-optimal: <70% beating cells per well in 75% of wells (as measured by Cellogy) at optimal seeding density (iPSC line-specific).	Inquire
D. Wnt Method: Cardiomyocyte Differentiation Kit	Reagents and media for cardiac differentiation of 1 iPSC line by the Wnt method. Includes 2-3 iPSC passaging, and cardiac differentiation to Day 15. Up to 8X 12-well plates.	Internal Gladstone $1,811.25 each UCSF $2,716.88 each Corporate $3,441.37 each External (non-profit) $4,528.12 each
E. Invisible Wnt Method: Cardiomyocyte Differentiation Service - Optimal Day 15 iPSC-cardiomyocytes	Service for efficient cardiomyocyte differentiations. Optimal: >60% beating cells per well in 75% of wells (as measured by Cellogy) at optimal seeding density (iPSC line-specific). Up to 8X 12-well plates. Includes all differentiation reagents.	Inquire
F. Invisible:Wnt Method: Cardiomyocyte Differentiation Service - Sub-optimal Day 15 iPSC-cardiomyocytes	Costs for inefficient cardiomyocyte differentiations. Suboptimal: >60% beating cells per well in 75% of wells (as measured by Cellogy) at optimal seeding density (iPSC line-specific). Up to 8X 12-well plates.	Inquire
G. Invisible:Wnt Method: Cardiomyocyte Density Optimization Service	Density optimization for cardiomyocyte differentiation of new cell lines. Up to 2x 12-well plates will be set up with a range of 3-4 densities to determine optimal seeding density for new cell lines.	Inquire
▼ ► Cells (5)
Name	Description	Price
Growth Inactivated SNL - 1x10^6 cells/vial		Inquire
Growth Inactivated SNL - 3x10^6 cells/vial		Inquire
Human iPSC line: CRISPRi Gen2C, frozen cryovial	Tetracycline-inducible CRISPR interference system in WTC11 human iPSC line. Contains the Tet-on-3G system driving expression of dCas9-KRAB (in one allele of the AAVS1 locus) in the human WTC iPSC line. With doxycycline addition, dCas9-KRAB is expressed. The other AAVS1 allele is wildtype. This cell line has no fluorophores. It is useful for repressing specific genes in human iPSCs and in early cell lineages. 1e6 cells per vial. Passage 33.14. Normal karyotype at passage 33.14. Reference:CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs. Mandegar, MA, Huebsch, N, Frolov, EB, Shin, E, Truong, A, Olvera, MP, Chan, AH, Miyaoka, Y, Holmes, K, Spencer, CI, Judge, LM, Gordon, DE, Eskildsen, TV5, Villalta, JE, Horlbeck, MA, Gilbert, LA, Krogan, NJ, Sheikh, SP, Weissman, JS, Qi, LS, So, PL, Conklin BR. Cell Stem Cell. 2016 Apr 7;18(4):541-53. PMID: 26971820 NOTE: For external requests, a material transfer agreement with Gladstone is required. Please contact stemcell-core@gladstone.ucsf.edu	Inquire
Z. (INVISIBLE) Human iPSC line: CRISPRi Gen1C, frozen cryovial	Invisible Tetracycline-inducible CRISPR interference system in WTC11 human iPSC line. Contains the Tet-on-3G system driving expression of dCas9-KRAB and mCherry (from one allele of the AAVS1 locus) in the human WTC iPSC line. With doxycycline addition, dCas9-KRAB and mCherry are expressed. In the other AAVS1 locus, there is a calcium biosensor, GCaMP6f, which is constitutively expressed. From Dr. Bruce Conklin's cell line collection. 1e6 cells per vial. Passage 47. Normal karyotype at passage 48. Reference: CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs. Mandegar, MA, Huebsch, N, Frolov, EB, Shin, E, Truong, A, Olvera, MP, Chan, AH, Miyaoka, Y, Holmes, K, Spencer, CI, Judge, LM, Gordon, DE, Eskildsen, TV5, Villalta, JE, Horlbeck, MA, Gilbert, LA, Krogan, NJ, Sheikh, SP, Weissman, JS, Qi, LS, So, PL, Conklin BR. Cell Stem Cell. 2016 Apr 7;18(4):541-53. PMID: 26971820 NOTE: For external requests, a material transfer agreement with Gladstone is required. Please contact stemcell-core@gladstone.ucsf.edu	Inquire
Z. INVISIBLE - Human iPSC line: CRISPRi Gen1B - frozen cryovial	Tetracycline-inducible CRISPR interference system in WTB human iPSC line. Contains the Tet-on-3G system driving expression of dCas9-KRAB. From Dr. Bruce Conklin's cell line collection. Reference: CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs. Mandegar, MA, Huebsch, N, Frolov, EB, Shin, E, Truong, A, Olvera, MP, Chan, AH, Miyaoka, Y, Holmes, K, Spencer, CI, Judge, LM, Gordon, DE, Eskildsen, TV5, Villalta, JE, Horlbeck, MA, Gilbert, LA, Krogan, NJ, Sheikh, SP, Weissman, JS, Qi, LS, So, PL, Conklin BR. Cell Stem Cell. 2016 Apr 7;18(4):541-53. PMID: 26971820. NOTE: For external requests, a material transfer agreement with Gladstone is required. Please contact stemcell-core@gladstone.ucsf.edu	Inquire
▼ ► Custom Projects (1)
Name	Description	Price
Z. Custom IPSC reprogramming from fibroblasts (all 3 phases) for Bridgebio		Inquire
▼ ► Services (9)
Name	Description	Price
1a. Mycoplasma screening - offered every Friday	Drop off samples to Room 428 before 1pm on Fridays. Please contact stemcell-core@gladstone.ucsf.edu for any questions about sample requirements.	Internal Gladstone $26.87 each UCSF $38.69 each Corporate $67.17 each External (non-profit) $51.00 each
1b. Mycoplasma Quarterly screening	Drop off samples to Room 428 before 1pm on the designated quarterly mycoplasma screening day. Please contact stemcell-core@gladstone.ucsf.edu for any questions about sample requirements.	Internal Gladstone $20.00 each UCSF $28.80 each Corporate $60.00 each
Genome engineering iPSC line, Service A: Custom CRISPR gene knockout by NHEJ - FULL SERVICE: includes labor, reagents, equipment use	Clonal homozygous gene KO iPSC line with >80% ICE KO score (2 rounds of manual single cell clone picking) Deliverables: 2 subclones of 2 independent clonal iPSC lines with >80% ICE KO score (pure) 3 sgRNA used for gene editing 1 working primer pair for genotyping 1 working Sanger sequencing primer hiPSC line - WTC (optional) Progress report If no clones are isolated, another attempt will be made at no extra cost. For more information, please contact stemcell-core@gladstone.ucsf.edu	Internal Gladstone $7,000.00 each UCSF $9,563.00 each Corporate $17,077.50 each External (non-profit) $12,978.00 each
Hypoxic Incubator - Weekly use	Weekly fee for use of the hypoxic incubator in room 461. Charge covers the cost of Nitrogen and incubator upkeep. Contact SCC staff at least one week in advance before needing to use the hypoxic incubator!	Inquire
iPSC line expansion and banking	Includes price of media and plastics, labor. You will receive 10 cryovials of 1 million cells/vial. Please provide karyotyped cells.	Internal Gladstone $1,000.00 each UCSF $1,440.00 each Corporate $2,500.00 each External (non-profit) $1,900.00 each
iPSC line expansion and banking - ADD ON Cleaning iPSC lines by manual picking of colonies and expansion	Add on to standard iPSC expansion and banking service for cell lines with significant spontaneous differentiation. Colonies with good iPCS morphology will be manually picked and expanded.You will receive 10 cryovials of 1 million cells/vial. Please provide karyotyped cells.	Inquire
PBMC isolation from whole blood /tube- RUSH Processing	Service includes isolation of PBMCs from fresh whole blood provided in CPT™ Vacutainer® (BD, Cat # 362761). Price per Vacutainer®. PBMCs will be frozen at 5e6 cells/vial unless otherwise specified. Contact the Stem Cell Core (stemcell-core@gladstone.ucsf.edu) prior to obtaining blood samples to schedule this service. Select this service for samples dropped off without at least one week's advance notice.	Inquire
PBMC isolation from whole blood/sample	Service includes isolation of PBMCs from fresh whole blood provided in CPT™ Vacutainer® (BD, Cat # 362761). Price per Vacutainer®. PBMCs will be frozen at 5e6 cells/vial unless otherwise specified. Contact the Stem Cell Core (stemcell-core@gladstone.ucsf.edu) at least one week prior to obtaining blood samples to schedule this service.	Internal Gladstone $350.00 each UCSF $504.00 each Corporate $875.00 each External (non-profit) $665.00 each
Project Consultation - Gene editing	Expert advice and consultation for iPSC and gene editing projects. The first consulation (~1h) is free-of-charge. Contact: stemcell-core@gladstone.ucsf.edu	Inquire
▼ ► Shipping - CLG (Cell Line Genetics) (2)
Name	Description	Price
Basic Cell Line Identity Testing - 24 Codis STR Loci and Amelogenin - Live Culture	Service available Tuesdays (holidays excluded) before 2:30 p.m.	Inquire
Cytogenetics service - G band Karyotyping - per cell line	Service available Tuesdays (holidays excluded) before 2:30 p.m. Submission Guidelines: Provide a 60-70% confluent T25 with plug-sealed cap filled entirely with media (no bubble) supplemented with 10%-15% FBS to increase cell survival during shipment. Also provide an additional 15 mL of media. Wrap the caps of both with parafilm. Fill out the CLG request sheet. Provide Core staff with request sheet and cells before 2:30 p.m.	Internal Gladstone $460.00 each
▼ ► Training Services (4)
Name	Description	Price
1. Human iPSC Culture Training - per person	This is a hands-on training by expert core staff using a control iPSC line WTC (or an equivalent line). Training will cover how to thaw, passage, and freeze human iPSCs, along with sterile tissue culture techniques. Price includes reagents, media, and hands-on instruction. (Approximately 2 weeks.)	Inquire
2. iPSC Colony Picking Training	This is a hands-on training by expert core staff using a control iPSC line WTC (or an equivalent line). Training will cover how to manually pick iPSC colonies for gene editing or reprogramming. Price includes reagents, media, and hands-on instruction. (Duration: 2 days)	Inquire
3. Cardiac Differentiation Training	This is a hands-on training by expert core staff using a control iPSC line WTC (or an equivalent line known to differentiate). Training will cover cardiac differentiation using the Wnt method up to Day 15. Price includes reagents, media, and hands-on instruction. (Approximately 3 weeks.)	Inquire
4. iPSC Reprogramming Training - Episomal Method from PBMCs	This is a hands-on training by expert core staff using control PBMCs (that have previously been successfully reprogrammed) and an experimental PBMC sample (requestor's choice). Training will cover: - Transfection of PBMCs with plasmids containing Yamanaka factors - Plating and maintenance of transfected cells - Identification of newly formed iPSC colonies - Picking iPS colonies onto matrigel coated wells - Training to expand and freeze iPSCs for viable recovery Price includes reagents, media, and hands-on instruction. (Approximately 1-2 months.)	Inquire

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